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1.
High level transient production of recombinant antibodies and antibody fusion proteins in HEK293 cells
Volker Jäger, Konrad Büssow, Andreas Wagner, Susanne Weber, Michael Hust, André Frenzel, Thomas Schirrmann BMC Biotechnology 2013, 13 :52 (26 June 2013)
Abstract | Full text | PDF | ePUB | PubMed
2.
Self-assembled peptide amphiphiles function as multivalent binder with increased hemagglutinin affinity
Christine Hüttl, Cornelia Hettrich, Reinhard Miller, Bernd-Reiner Paulke, Petra Henklein, Harshadrai Rawel, Frank F Bier BMC Biotechnology 2013, 13 :51 (18 June 2013)
Abstract | Full text | PDF | ePUB | PubMed
3.
Towards a novel influenza vaccine: engineering of hemagglutinin on a platform of adenovirus dodecahedron
Antonina Naskalska, Ewa Szolajska, Igor Andreev, Malgorzata Podsiadla, Jadwiga Chroboczek BMC Biotechnology 2013, 13 :50 (16 June 2013)
Abstract | Full text | PDF | PubMed
4.
Characterization of a novel swollenin from Penicillium oxalicum in facilitating enzymatic saccharification of cellulose
Kang Kang, Shaowen Wang, Guohong Lai, Gang Liu, Miao Xing BMC Biotechnology 2013, 13 :42 (20 May 2013)
Abstract | Full text | PDF | ePUB | PubMed | Cited on BioMed Central
5.
Protein body formation in stable transgenic tobacco expressing elastin-like polypeptide and hydrophobin fusion proteins
Sonia P Gutiérrez, Reza Saberianfar, Susanne E Kohalmi, Rima Menassa BMC Biotechnology 2013, 13 :40 (10 May 2013)
Abstract | Full text | PDF | ePUB | PubMed
6.
Development of a recombinant antibody to target peptides and proteins to sialoadhesin-expressing macrophages
Karen Ooms, Hanne Van Gorp, Tim Van Gaever, Hans J Nauwynck, Peter L Delputte BMC Biotechnology 2013, 13 :33 (10 April 2013)
Abstract | Full text | PDF | ePUB | PubMed
7.
Baculovirus-mediated gene transfer in butterfly wings in vivo : an efficient expression system with an anti-gp64 antibody
Bidur Dhungel, Yoshikazu Ohno, Rie Matayoshi, Joji M Otaki BMC Biotechnology 2013, 13 :27 (25 March 2013)
Abstract | Full text | PDF | ePUB | PubMed
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Editor’s summary
A novel baculovirus-mediated gene transfer system is an efficient tool to transfer, express, and functionally examine foreign genes in butterfly wings and may be useful in other non-model insect systems.
8.
Streptomyces spp. as efficient expression system for a d ,d -peptidase/d ,d -carboxypeptidase involved in glycopeptide antibiotic resistance
Elisa Binda, Giorgia Letizia Marcone, Francesca Berini, Loredano Pollegioni, Flavia Marinelli BMC Biotechnology 2013, 13 :24 (16 March 2013)
Abstract | Full text | PDF | ePUB | PubMed
9.
Characterization of a newly identified rice chitinase-like protein (OsCLP) homologous to xylanase inhibitor
Jingni Wu, Yiming Wang, Sun Tae Kim, Sang Gon Kim, Kyu Young Kang BMC Biotechnology 2013, 13 :4 (18 January 2013)
Abstract | Full text | PDF | PubMed
10.
Cloning, overexpression, purification, and characterization of a polyextremophilic β-galactosidase from the Antarctic haloarchaeon Halorubrum lacusprofundi
Ram Karan, Melinda D Capes, Priya DasSarma, Shiladitya DasSarma BMC Biotechnology 2013, 13 :3 (16 January 2013)
Abstract | Full text | PDF | PubMed
11.
A simplified counter-selection recombineering protocol for creating fluorescent protein reporter constructs directly from C. elegans fosmid genomic clones
Nisha Hirani, Marcel Westenberg, Minaxi S Gami, Paul Davis, Ian A Hope, Colin T Dolphin BMC Biotechnology 2013, 13 :1 (3 January 2013)
Abstract | Full text | PDF | PubMed
12.
A vaccine grade of yeast Saccharomyces cerevisiae expressing mammalian myostatin
Tingting Zhang, Lin Sun, Ying Xin, Lixia Ma, Youyou Zhang, Xin Wang, Kun Xu, Chonghua Ren, Cunfang Zhang, Zhilong Chen, Hanjiang Yang, Zhiying Zhang BMC Biotechnology 2012, 12 :97 (19 December 2012)
Abstract | Full text | PDF | PubMed
13.
Expression of recombinant staphylokinase in the methylotrophic yeast Hansenula polymorpha
Manal Moussa, Mahmoud Ibrahim, Maria El Ghazaly, Jan Rohde, Stefan Gnoth, Andreas Anton, Frank Kensy, Frank Mueller BMC Biotechnology 2012, 12 :96 (19 December 2012)
Abstract | Full text | PDF | PubMed
14.
Guanylate-binding protein 1 expression from embryonal endothelial progenitor cells reduces blood vessel density and cellular apoptosis in an axially vascularised tissue-engineered construct
Oliver Bleiziffer, Matthias Hammon, Andreas Arkudas, Christian D Taeger, Justus P Beier, Kerstin Amann, Elisabeth Naschberger, Michael Stürzl, Raymund E Horch, Ulrich Kneser BMC Biotechnology 2012, 12 :94 (6 December 2012)
Abstract | Full text | PDF | ePUB | PubMed
15.
Characterization of transferrin receptor-mediated endocytosis and cellular iron delivery of recombinant human serum transferrin from rice (Oryza sativa L.)
Deshui Zhang, Hsin-Fang Lee, Steven C Pettit, Jennica L Zaro, Ning Huang, Wei-Chiang Shen BMC Biotechnology 2012, 12 :92 (30 November 2012)
Abstract | Full text | PDF | PubMed
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Editor’s summary
Rice-derived recombinant human transferrin (rhTF) demonstrates similar transferrin receptor (TFR) binding, TFR-mediated endocytosis and cellular iron delivery function to human transferrin (hTF) and may represent a useful, low-cost alternative for biopharmaceutical applications.
16.
Generation and functional characterization of the anti-transferrin receptor single-chain antibody-GAL4 (TfRscFv-GAL4) fusion protein
Qing Ye, Heyu Hu, Zhihua Wang, Tong Lu, Zhiquan Hu, Xing Zeng, Shu Zhang, Jing Liu, Ping Lei, Cong-Yi Wang, Zhangqun Ye, Guanxin Shen BMC Biotechnology 2012, 12 :91 (28 November 2012)
Abstract | Full text | PDF | PubMed
17.
Using protein microarray technology to screen anti-ERCC1 monoclonal antibodies for specificity and applications in pathology
Donghui Ma, Dror Baruch, Youmin Shu, Kehu Yuan, Zairen Sun, Kaiyan Ma, Toan Hoang, Wei Fu, Li Min, Zhu-Sheng Lan, Fangxun Wang, Lori Mull, Wei-Wu He BMC Biotechnology 2012, 12 :88 (21 November 2012)
Abstract | Full text | PDF | PubMed
18.
A novel strategy for efficient production of anti-V3 human scFvs against HIV-1 clade C
Rajesh Kumar, Raiees Andrabi, Ashutosh Tiwari, Somi Sankaran Prakash, Naveet Wig, Durgashree Dutta, Anurag Sankhyan, Lubina Khan, Subrata Sinha, Kalpana Luthra BMC Biotechnology 2012, 12 :87 (15 November 2012)
Abstract | Full text | PDF | PubMed
19.
Antigen vehiculization particles based on the Z protein of Junin virus
Cristina S Borio, Marcos F Bilen, Marcelo H Argüelles, Sandra E Goñi, Javier A Iserte, Graciela Glikmann, Mario E Lozano BMC Biotechnology 2012, 12 :80 (2 November 2012)
Abstract | Full text | PDF | PubMed
20.
Live attenuated influenza viruses produced in a suspension process with avian AGE1.CR.pIX cells
Verena Lohr, Yvonne Genzel, Ingo Jordan, Dietmar Katinger, Stefan Mahr, Volker Sandig, Udo Reichl BMC Biotechnology 2012, 12 :79 (30 October 2012)
Abstract | Full text | PDF | PubMed
21.
Pandemic influenza vaccine: characterization of A/California/07/2009 (H1N1) recombinant hemagglutinin protein and insights into H1N1 antigen stability
Elena Feshchenko, David G Rhodes, Rachael Felberbaum, Clifton McPherson, Joseph A Rininger, Penny Post, Manon MJ Cox BMC Biotechnology 2012, 12 :77 (30 October 2012)
Abstract | Full text | PDF | PubMed
22.
Aggravation of cold-induced injury in Vero-B4 cells by RPMI 1640 medium – Identification of the responsible medium components
Gesine Pless-Petig, Martin Metzenmacher, Tobias R Türk, Ursula Rauen BMC Biotechnology 2012, 12 :73 (10 October 2012)
Abstract | Full text | PDF | PubMed
23.
Identification of a novel enhancer that binds Sp1 and contributes to induction of cold-inducible RNA-binding protein (cirp) expression in mammalian cells
Yasuhiko Sumitomo, Hiroaki Higashitsuji, Hisako Higashitsuji, Yu Liu, Takanori Fujita, Toshiharu Sakurai, Marco M Candeias, Katsuhiko Itoh, Tsutomu Chiba, Jun Fujita BMC Biotechnology 2012, 12 :72 (10 October 2012)
Abstract | Full text | PDF | PubMed
24.
Enhanced cell disruption strategy in the release of recombinant hepatitis B surface antigen from Pichia pastoris using response surface methodology
Yew Tam, Zeenathul Allaudin, Mohd Azmi Lila, Abdul Bahaman, Joo Tan, Morvarid Rezaei BMC Biotechnology 2012, 12 :70 (5 October 2012)
Abstract | Full text | PDF | PubMed
25.
Selective delivery of interleukine-1 receptor antagonist to inflamed joint by albumin fusion
Mengyuan Liu, Yi Huang, Lei Hu, Guoping Liu, Xueping Hu, Dongxu Liu, Xiaosong Yang BMC Biotechnology 2012, 12 :68 (25 September 2012)
Abstract | Full text | PDF | PubMed