All
1.
Characterization of a newly identified rice chitinase-like protein (OsCLP) homologous to xylanase inhibitor
Jingni Wu, Yiming Wang, Sun Tae Kim, Sang Gon Kim, Kyu Young Kang BMC Biotechnology 2013, 13 :4 (18 January 2013)
Abstract | Full text | PDF | PubMed
2.
Cloning, overexpression, purification, and characterization of a polyextremophilic β -galactosidase from the Antarctic haloarchaeon Halorubrum lacusprofundi
Ram Karan, Melinda D Capes, Priya DasSarma, Shiladitya DasSarma BMC Biotechnology 2013, 13 :3 (16 January 2013)
Abstract | Full text | PDF | PubMed
3.
A simplified counter-selection recombineering protocol for creating fluorescent protein reporter constructs directly from C. elegans fosmid genomic clones
Nisha Hirani, Marcel Westenberg, Minaxi S Gami, Paul Davis, Ian A Hope, Colin T Dolphin BMC Biotechnology 2013, 13 :1 (3 January 2013)
Abstract | Full text | PDF | PubMed
4.
A vaccine grade of yeast Saccharomyces cerevisiae expressing mammalian myostatin
Tingting Zhang, Lin Sun, Ying Xin, Lixia Ma, Youyou Zhang, Xin Wang, Kun Xu, Chonghua Ren, Cunfang Zhang, Zhilong Chen, Hanjiang Yang, Zhiying Zhang BMC Biotechnology 2012, 12 :97 (19 December 2012)
Abstract | Full text | PDF | PubMed
5.
Expression of recombinant staphylokinase in the methylotrophic yeast Hansenula polymorpha
Manal Moussa, Mahmoud Ibrahim, Maria El Ghazaly, Jan Rohde, Stefan Gnoth, Andreas Anton, Frank Kensy, Frank Mueller BMC Biotechnology 2012, 12 :96 (19 December 2012)
Abstract | Full text | PDF | PubMed
6.
Characterization of transferrin receptor-mediated endocytosis and cellular iron delivery of recombinant human serum transferrin from rice (Oryza sativa L.)
Deshui Zhang, Hsin-Fang Lee, Steven C Pettit, Jennica L Zaro, Ning Huang, Wei-Chiang Shen BMC Biotechnology 2012, 12 :92 (30 November 2012)
Abstract | Full text | PDF | PubMed
|
Editor’s summary
Rice-derived recombinant human transferrin (rhTF) demonstrates similar transferrin receptor (TFR) binding, TFR-mediated endocytosis and cellular iron delivery function to human transferrin (hTF) and may represent a useful, low-cost alternative for biopharmaceutical applications.
7.
Generation and functional characterization of the anti-transferrin receptor single-chain antibody-GAL4 (TfRscFv-GAL4) fusion protein
Qing Ye, Heyu Hu, Zhihua Wang, Tong Lu, Zhiquan Hu, Xing Zeng, Shu Zhang, Jing Liu, Ping Lei, Cong-Yi Wang, Zhangqun Ye, Guanxin Shen BMC Biotechnology 2012, 12 :91 (28 November 2012)
Abstract | Full text | PDF | PubMed
8.
Using protein microarray technology to screen anti-ERCC1 monoclonal antibodies for specificity and applications in pathology
Donghui Ma, Dror Baruch, Youmin Shu, Kehu Yuan, Zairen Sun, Kaiyan Ma, Toan Hoang, Wei Fu, Li Min, Zhu-Sheng Lan, Fangxun Wang, Lori Mull, Wei-Wu He BMC Biotechnology 2012, 12 :88 (21 November 2012)
Abstract | Full text | PDF | PubMed
9.
A novel strategy for efficient production of anti-V3 human scFvs against HIV-1 clade C
Rajesh Kumar, Raiees Andrabi, Ashutosh Tiwari, Somi Sankaran Prakash, Naveet Wig, Durgashree Dutta, Anurag Sankhyan, Lubina Khan, Subrata Sinha, Kalpana Luthra BMC Biotechnology 2012, 12 :87 (15 November 2012)
Abstract | Full text | PDF | PubMed
10.
Antigen vehiculization particles based on the Z protein of Junin virus
Cristina S Borio, Marcos F Bilen, Marcelo H Argüelles, Sandra E Goñi, Javier A Iserte, Graciela Glikmann, Mario E Lozano BMC Biotechnology 2012, 12 :80 (2 November 2012)
Abstract | Full text | PDF | PubMed
11.
Live attenuated influenza viruses produced in a suspension process with avian AGE1.CR.pIX cells
Verena Lohr, Yvonne Genzel, Ingo Jordan, Dietmar Katinger, Stefan Mahr, Volker Sandig, Udo Reichl BMC Biotechnology 2012, 12 :79 (30 October 2012)
Abstract | Full text | PDF | PubMed
12.
Pandemic influenza vaccine: characterization of A/California/07/2009 (H1N1) recombinant hemagglutinin protein and insights into H1N1 antigen stability
Elena Feshchenko, David G Rhodes, Rachael Felberbaum, Clifton McPherson, Joseph A Rininger, Penny Post, Manon MJ Cox BMC Biotechnology 2012, 12 :77 (30 October 2012)
Abstract | Full text | PDF | PubMed
13.
Aggravation of cold-induced injury in Vero-B4 cells by RPMI 1640 medium – Identification of the responsible medium components
Gesine Pless-Petig, Martin Metzenmacher, Tobias R Türk, Ursula Rauen BMC Biotechnology 2012, 12 :73 (10 October 2012)
Abstract | Full text | PDF | PubMed
14.
Identification of a novel enhancer that binds Sp1 and contributes to induction of cold-inducible RNA-binding protein (cirp) expression in mammalian cells
Yasuhiko Sumitomo, Hiroaki Higashitsuji, Hisako Higashitsuji, Yu Liu, Takanori Fujita, Toshiharu Sakurai, Marco M Candeias, Katsuhiko Itoh, Tsutomu Chiba, Jun Fujita BMC Biotechnology 2012, 12 :72 (10 October 2012)
Abstract | Full text | PDF | PubMed
15.
Enhanced cell disruption strategy in the release of recombinant hepatitis B surface antigen from Pichia pastoris using response surface methodology
Yew Tam, Zeenathul Allaudin, Mohd Azmi Lila, Abdul Bahaman, Joo Tan, Morvarid Rezaei BMC Biotechnology 2012, 12 :70 (5 October 2012)
Abstract | Full text | PDF | PubMed
16.
Selective delivery of interleukine-1 receptor antagonist to inflamed joint by albumin fusion
Mengyuan Liu, Yi Huang, Lei Hu, Guoping Liu, Xueping Hu, Dongxu Liu, Xiaosong Yang BMC Biotechnology 2012, 12 :68 (25 September 2012)
Abstract | Full text | PDF | PubMed
17.
Structural insights from random mutagenesis of Campylobacter jejuni oligosaccharyltransferase PglB
Julian Ihssen, Michael Kowarik, Luzia Wiesli, Renate Reiss, Michael Wacker, Linda Thöny-Meyer BMC Biotechnology 2012, 12 :67 (24 September 2012)
Abstract | Full text | PDF | PubMed
18.
Bipartite and tripartite Cucumber mosaic virus -based vectors for producing the Acidothermus cellulolyticus endo-1,4-β -glucanase and other proteins in non-transgenic plants
Min Hwang, Benjamin E Lindenmuth, Karen A McDonald, Bryce W Falk BMC Biotechnology 2012, 12 :66 (21 September 2012)
Abstract | Full text | PDF | PubMed
19.
Short-chain fluorescent tryptophan tags for on-line detection of functional recombinant proteins
Eva-Maria Siepert, Esther Gartz, Mehmet Kemal Tur, Heinrich Delbrück, Stefan Barth, Jochen Büchs BMC Biotechnology 2012, 12 :65 (21 September 2012)
Abstract | Full text | PDF | PubMed
20.
An efficient strategy for cell-based antibody library selection using an integrated vector system
Hyerim Yoon, Jin Song, Chun Ryu, Yeon-Gu Kim, Eun Lee, Sunghyun Kang, Sang Kim BMC Biotechnology 2012, 12 :62 (18 September 2012)
Abstract | Full text | PDF | PubMed
21.
Functional enhancement of neuronal cell behaviors and differentiation by elastin-mimetic recombinant protein presenting Arg-Gly-Asp peptides
Won Jeon, Bo Park, Seong Choi, Kyeong-Min Lee, Jin-Kyu Park BMC Biotechnology 2012, 12 :61 (14 September 2012)
Abstract | Full text | PDF | PubMed
|
Editor’s summary
An elastin-mimetic protein consisting of alternating tropoelastin structural domains and cell-binding Arg-Gly-Asp motifs stimulates neuronal cell behaviors and differentiation in N2a neuroblastoma cells, both highly desirable characteristic in neural development and nerve repair.
22.
Perlecan domain 1 recombinant proteoglycan augments BMP-2 activity and osteogenesis
Arthur A DeCarlo, Maria Belousova, April L Ellis, Donald Petersen, Hernan Grenett, Patrick Hardigan, Robert O’Grady, Megan Lord, John M Whitelock BMC Biotechnology 2012, 12 :60 (11 September 2012)
Abstract | Full text | PDF | PubMed
23.
Rotavirus A-specific single-domain antibodies produced in baculovirus-infected insect larvae are protective in vivo
Silvia Gómez-Sebastián, Maria C Nuñez, Lorena Garaicoechea, Carmen Alvarado, Marina Mozgovoj, Rodrigo Lasa, Alan Kahl, Andres Wigdorovitz, Viviana Parreño, José M Escribano BMC Biotechnology 2012, 12 :59 (7 September 2012)
Abstract | Full text | PDF | PubMed
24.
High yield expression of catalytically active USP18 (UBP43) using a Trigger Factor fusion system
Anja Basters, Lars Ketscher, Elke Deuerling, Christoph Arkona, Jörg Rademann, Klaus-Peter Knobeloch, Günter Fritz BMC Biotechnology 2012, 12 :56 (23 August 2012)
Abstract | Full text | PDF | PubMed
25.
A set of dual promoter vectors for high throughput cloning, screening, and protein expression in eukaryotic and prokaryotic systems from a single plasmid
Namita Sinah, Charlotte A Williams, Robert C Piper, S Shields BMC Biotechnology 2012, 12 :54 (23 August 2012)
Abstract | Full text | PDF | PubMed