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BMC Biotechnology

Most viewed

Editor-in-Chief
Melissa Norton, MD

Biology Editor
Penelope Webb, PhD

In-house Editor
J. Ann Le Good



BMC  BMC Biotechnology: Most viewed articles of all time


Figures given indicate the abstract, full text and PDF accesses on BioMed Central for each article, for all time. All research articles are also available via the PubMed Central archive, so the total number of accesses to each article is significantly higher. Lists of most-viewed articles over the last 30 days and most-viewed articles in the past year are also available.

all time most-viewed articles from all BioMed Central Journals

1.
Accesses
44568
Methodology article
Real-time PCR based on SYBR-Green I fluorescence: An alternative to the TaqMan assay for a relative quantification of gene rearrangements, gene amplifications and micro gene deletions
Frederique Ponchel, Carmel Toomes, Kieran Bransfield, Fong T Leong, Susan H Douglas, Sarah L Field, Sandra M Bell, Valerie Combaret, Alain Puisieux, Alan J Mighell, Philip A Robinson, Chris F Inglehearn, John D Isaacs, Alex F Markham
BMC Biotechnology 2003, 3:18 (13October2003)
[Abstract] [Full text] [PDF] [PubMed] [Related articles] [Cited on BioMed Central]

2.
Accesses
34987
Methodology article
Retrovirus-delivered siRNA
Eric Devroe, Pamela A Silver
BMC Biotechnology 2002, 2:15 (28August2002)
[Abstract] [Full text] [PDF] [PubMed] [Related articles] [Cited on BioMed Central]

3.
Accesses
30409
Research article
Effective generation of transgenic pigs and mice by linker based sperm-mediated gene transfer.
Keejong Chang, Jin Qian, MeiSheng Jiang, Yi-Hsin Liu, Ming-Che Wu, Chi-Dar Chen, Chao-Kuen Lai, Hsin-Lung Lo, Chin-Ton Hsiao, Lucy Brown, James Bolen Jr, Hsiao-I Huang, Pei-Yu Ho, Ping Yao Shih, Chen-Wen Yao, Wey-Jinq Lin, Chung-Hsi Chen, Fang-Yi Wu, Yi-Jen Lin, Jing Xu, Ken Wang
BMC Biotechnology 2002, 2:5 (19April2002)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

4.
Accesses
21214
Methodology article
An automated method for high-throughput protein purification applied to a comparison of His-tag and GST-tag affinity chromatography
Christoph Scheich, Volker Sievert, Konrad Bssow
BMC Biotechnology 2003, 3:12 (28July2003)
[Abstract] [Full text] [PDF] [PubMed] [Related articles] [Cited on BioMed Central]

5.
Accesses
20163
Methodology article
Performance of high-throughput DNA quantification methods
Kashif A Haque, Ruth M Pfeiffer, Michael B Beerman, Jeff P Struewing, Stephen J Chanock, Andrew W Bergen
BMC Biotechnology 2003, 3:20 (28October2003)
[Abstract] [Full text] [PDF] [PubMed] [Related articles] [Cited on BioMed Central]

6.
Accesses
19698
Methodology article
Mutant loxP vectors for selectable marker recycle and conditional knock-outs
Hiroshi Arakawa, Dimitry Lodygin, Jean-Marie Buerstedde
BMC Biotechnology 2001, 1:7 (26September2001)
[Abstract] [Full text] [PDF] [PubMed] [Related articles] [Cited on BioMed Central]

7.
Accesses
16292
Methodology article
Use of polyethyleneimine polymer in cell culture as attachment factor and lipofection enhancer
Ajith R Vancha, Suman Govindaraju, Kishore VL Parsa, Madhuri Jasti, Maribel Gonzlez-Garca, Rafael P Ballestero
BMC Biotechnology 2004, 4:23 (15October2004)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

8.
Accesses
15741

Methodology article
A high-throughput cell migration assay using scratch wound healing, a comparison of image-based readout methods
Justin C Yarrow, Zachary E Perlman, Nicholas J Westwood, Timothy J Mitchison
BMC Biotechnology 2004, 4:21 (9September2004)
[Abstract] [Full text] [PDF] [PubMed] [Related articles] [F1000 Biology] [Cited on BioMed Central]

9.
Accesses
15436
Methodology article
Embryonic stem cells and mice expressing different GFP variants for multiple non-invasive reporter usage within a single animal
Anna-Katerina Hadjantonakis, Suzanne Macmaster, Andras Nagy
BMC Biotechnology 2002, 2:11 (11June2002)
[Abstract] [Full text] [PDF] [PubMed] [Related articles] [Cited on BioMed Central]

10.
Accesses
15211
Methodology article
A comparison between two brine shrimp assays to detect in vitro cytotoxicity in marine natural products
Jos Luis Carballo, Zaira L Hernndez-Inda, Pilar Prez, Mara D Garca-Grvalos
BMC Biotechnology 2002, 2:17 (23September2002)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

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