Most viewed
Editor-in-Chief Melissa Norton, MD
Biology Editor Penelope Webb, PhD
In-house Editor J. Ann Le Good
BMC Biotechnology: Most viewed articles of all time
Figures given indicate the abstract, full text and PDF accesses on BioMed Central for each article, for all time. All research articles are also available via the PubMed Central archive, so the total number of accesses to each article is significantly higher. Lists of
most-viewed articles over the last 30 days and
most-viewed articles in the past year are also available.
all time most-viewed articles from all BioMed Central Journals
1. Accesses 44568
Methodology article
Real-time PCR based on SYBR-Green I fluorescence: An alternative to the TaqMan assay for a relative quantification of gene rearrangements, gene amplifications and micro gene deletions Frederique Ponchel, Carmel Toomes, Kieran Bransfield, Fong T Leong, Susan H Douglas, Sarah L Field, Sandra M Bell, Valerie Combaret, Alain Puisieux, Alan J Mighell, Philip A Robinson, Chris F Inglehearn, John D Isaacs, Alex F Markham BMC Biotechnology 2003, 3 :18 (13 October 2003) [Abstract ] [Full text ] [PDF ] [PubMed ] [Related articles ] [Cited on BioMed Central ]
2. Accesses 34987
Methodology article
Retrovirus-delivered siRNA Eric Devroe, Pamela A Silver BMC Biotechnology 2002, 2 :15 (28 August 2002) [Abstract ] [Full text ] [PDF ] [PubMed ] [Related articles ] [Cited on BioMed Central ]
3. Accesses 30409
Research article
Effective generation of transgenic pigs and mice by linker based sperm-mediated gene transfer. Keejong Chang, Jin Qian, MeiSheng Jiang, Yi-Hsin Liu, Ming-Che Wu, Chi-Dar Chen, Chao-Kuen Lai, Hsin-Lung Lo, Chin-Ton Hsiao, Lucy Brown, James Bolen Jr, Hsiao-I Huang, Pei-Yu Ho, Ping Yao Shih, Chen-Wen Yao, Wey-Jinq Lin, Chung-Hsi Chen, Fang-Yi Wu, Yi-Jen Lin, Jing Xu, Ken Wang BMC Biotechnology 2002, 2 :5 (19 April 2002) [Abstract ] [Full text ] [PDF ] [PubMed ] [Related articles ]
4. Accesses 21214
Methodology article
An automated method for high-throughput protein purification applied to a comparison of His-tag and GST-tag affinity chromatography Christoph Scheich, Volker Sievert, Konrad B ssow BMC Biotechnology 2003, 3 :12 (28 July 2003) [Abstract ] [Full text ] [PDF ] [PubMed ] [Related articles ] [Cited on BioMed Central ]
5. Accesses 20163
Methodology article
Performance of high-throughput DNA quantification methods Kashif A Haque, Ruth M Pfeiffer, Michael B Beerman, Jeff P Struewing, Stephen J Chanock, Andrew W Bergen BMC Biotechnology 2003, 3 :20 (28 October 2003) [Abstract ] [Full text ] [PDF ] [PubMed ] [Related articles ] [Cited on BioMed Central ]
6. Accesses 19698
Methodology article
Mutant loxP vectors for selectable marker recycle and conditional knock-outs Hiroshi Arakawa, Dimitry Lodygin, Jean-Marie Buerstedde BMC Biotechnology 2001, 1 :7 (26 September 2001) [Abstract ] [Full text ] [PDF ] [PubMed ] [Related articles ] [Cited on BioMed Central ]
7. Accesses 16292
Methodology article
Use of polyethyleneimine polymer in cell culture as attachment factor and lipofection enhancer Ajith R Vancha, Suman Govindaraju, Kishore VL Parsa, Madhuri Jasti, Maribel Gonz lez-Garc a, Rafael P Ballestero BMC Biotechnology 2004, 4 :23 (15 October 2004) [Abstract ] [Full text ] [PDF ] [PubMed ] [Related articles ]
8. Accesses 15741
Methodology article
A high-throughput cell migration assay using scratch wound healing, a comparison of image-based readout methods Justin C Yarrow, Zachary E Perlman, Nicholas J Westwood, Timothy J Mitchison BMC Biotechnology 2004, 4 :21 (9 September 2004) [Abstract ] [Full text ] [PDF ] [PubMed ] [Related articles ] [F1000 Biology ] [Cited on BioMed Central ]
9. Accesses 15436
Methodology article
Embryonic stem cells and mice expressing different GFP variants for multiple non-invasive reporter usage within a single animal Anna-Katerina Hadjantonakis, Suzanne Macmaster, Andras Nagy BMC Biotechnology 2002, 2 :11 (11 June 2002) [Abstract ] [Full text ] [PDF ] [PubMed ] [Related articles ] [Cited on BioMed Central ]
10. Accesses 15211
Methodology article
A comparison between two brine shrimp assays to detect in vitro cytotoxicity in marine natural products Jos Luis Carballo, Zaira L Hern ndez-Inda, Pilar P rez, Mar a D Garc a-Gr valos BMC Biotechnology 2002, 2 :17 (23 September 2002) [Abstract ] [Full text ] [PDF ] [PubMed ] [Related articles ]