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1.
1253 Accesses
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A new method to customize protein expression vectors for fast, efficient and background free parallel cloning
Judith Scholz, Hüseyin Besir, Claudia Strasser, Sabine Suppmann BMC Biotechnology 2013, 13:12 (14 February 2013)
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2.
1060 Accesses
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An efficient one-step site-directed deletion, insertion, single and multiple-site plasmid mutagenesis protocol
Huanting Liu, James H Naismith BMC Biotechnology 2008, 8:91 (4 December 2008)
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| PubMed | Cited on BioMed Central
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3.
1014 Accesses
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Protein body formation in stable transgenic tobacco expressing elastin-like polypeptide and hydrophobin fusion proteins
Sonia P Gutiérrez, Reza Saberianfar, Susanne E Kohalmi, Rima Menassa BMC Biotechnology 2013, 13:40 (10 May 2013)
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4.
924 Accesses
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Real-time PCR based on SYBR-Green I fluorescence: An alternative to the TaqMan assay for a relative quantification of gene rearrangements, gene amplifications and micro gene deletions
Frederique Ponchel, Carmel Toomes, Kieran Bransfield, Fong T Leong, Susan H Douglas, Sarah L Field, Sandra M Bell, Valerie Combaret, Alain Puisieux, Alan J Mighell, Philip A Robinson, Chris F Inglehearn, John D Isaacs, Alex F Markham BMC Biotechnology 2003, 3:18 (13 October 2003)
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| PubMed | Cited on BioMed Central
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5.
876 Accesses
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Characterization of a novel swollenin from Penicillium oxalicum in facilitating enzymatic saccharification of cellulose
Kang Kang, Shaowen Wang, Guohong Lai, Gang Liu, Miao Xing BMC Biotechnology 2013, 13:42 (20 May 2013)
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6.
740 Accesses
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Less is more: strategies to remove marker genes from transgenic plants
Yuan-Yeu Yau, C Neal Stewart BMC Biotechnology 2013, 13:36 (23 April 2013)
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Editor’s summary
Yuan-Yeu Yau and C Neal Stewart review existing strategies to remove selectable marker genes from transgenic plants, including site specific recombination systems, and explore emerging technologies for increased precision genome modification.
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7.
713 Accesses
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A high-throughput cell migration assay using scratch wound healing, a comparison of image-based readout methods
Justin C Yarrow, Zachary E Perlman, Nicholas J Westwood, Timothy J Mitchison BMC Biotechnology 2004, 4:21 (9 September 2004)
Abstract | Full text | PDF
| PubMed | Cited on BioMed Central
| F1000 Biology
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Editor’s summary
Adaptation of the scratch wound-healing assay to a 384 well-plate format enables a high throughput analysis of cell migration and other cell processes involved in wound healing.
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8.
707 Accesses
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FastCloning: a highly simplified, purification-free, sequence- and ligation-independent PCR cloning method
Chaokun Li, Aiyun Wen, Benchang Shen, Jia Lu, Yao Huang, Yongchang Chang BMC Biotechnology 2011, 11:92 (12 October 2011)
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9.
710 Accesses
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Bacillus pumilus laccase: a heat stable enzyme with a wide substrate spectrum
Renate Reiss, Julian Ihssen, Linda Thöny-Meyer BMC Biotechnology 2011, 11:9 (25 January 2011)
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| PubMed | Cited on BioMed Central
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10.
662 Accesses
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Strategy for successful expression of the Pseudomonas putida nitrile hydratase activator P14K in Escherichia coli
Yi Liu, Wenjing Cui, Yueqin Fang, Yuechun Yu, Youtian Cui, Yuanyuan Xia, Michihiko Kobayashi, Zhemin Zhou BMC Biotechnology 2013, 13:48 (3 June 2013)
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11.
675 Accesses
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Enhanced itaconic acid production in Aspergillus niger using genetic modification and medium optimization
An Li, Nina Pfelzer, Robbert Zuijderwijk, Peter Punt BMC Biotechnology 2012, 12:57 (27 August 2012)
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12.
704 Accesses
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Achieving pH control in microalgal cultures through fed-batch addition of stoichiometrically-balanced growth media
Megerle L Scherholz, Wayne R Curtis BMC Biotechnology 2013, 13:39 (7 May 2013)
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13.
716 Accesses
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In situ magnetic separation of antibody fragments from Escherichia coli in complex media
Martin Cerff, Alexander Scholz, Matthias Franzreb, Iris L Batalha, Ana Cecilia Roque, Clemens Posten BMC Biotechnology 2013, 13:44 (20 May 2013)
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14.
614 Accesses
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Expression and efficient secretion of a functional chitinase from Chromobacterium violaceum in Escherichia coli
Marina Duarte Lobo, Fredy Davi Silva, Patrícia Gadelha Landim, Paloma Ribeiro da Cruz, Thaís Lima de Brito, Suelen Carneiro de Medeiros, José Tadeu Oliveira, Ilka Maria Vasconcelos, Humberto D¿Muniz Pereira, Thalles Barbosa Grangeiro BMC Biotechnology 2013, 13:46 (1 June 2013)
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15.
625 Accesses
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Improved mycobacterial protein production using a Mycobacterium smegmatis groEL1ΔC expression strain
Elke E Noens, Chris Williams, Madhankumar Anandhakrishnan, Christian Poulsen, Matthias T Ehebauer, Matthias Wilmanns BMC Biotechnology 2011, 11:27 (25 March 2011)
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16.
622 Accesses
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Optimization of culture conditions to produce high yields of active Acetobacter sp. CCTCC M209061 cells for anti-Prelog reduction of prochiral ketones
Xiao-Hong Chen, Wen-Yong Lou, Min-Hua Zong, Thomas J Smith BMC Biotechnology 2011, 11:110 (20 November 2011)
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| PubMed | Cited on BioMed Central
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17.
590 Accesses
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Metabolic evolution of Corynebacterium glutamicum for increased production of L-ornithine
Ling-Yan Jiang, Shang-Guang Chen, Yuan-Yuan Zhang, Jian-Zhong Liu BMC Biotechnology 2013, 13:47 (1 June 2013)
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18.
567 Accesses
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Towards a novel influenza vaccine: engineering of hemagglutinin on a platform of adenovirus dodecahedron
Antonina Naskalska, Ewa Szolajska, Igor Andreev, Malgorzata Podsiadla, Jadwiga Chroboczek BMC Biotechnology 2013, 13:50 (16 June 2013)
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19.
589 Accesses
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Generation of a tumor- and tissue-specific episomal non-viral vector system
Rudolf Haase, Terese Magnusson, Baowei Su, Florian Kopp, Ernst Wagner, Hans Lipps, Armin Baiker, Manfred Ogris BMC Biotechnology 2013, 13:49 (4 June 2013)
Abstract | Provisional PDF
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20.
571 Accesses
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Optimization and validation of mitochondria-based functional assay as a useful tool to identify BH3-like molecules selectively targeting anti-apoptotic Bcl-2 proteins
Jianting Long, Liu Liu, Zaneta Nikolovska-Coleska, Sanjeev Shangary, Han Yi, Shenming Wang, Shaomeng Wang BMC Biotechnology 2013, 13:45 (24 May 2013)
Abstract | Provisional PDF
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21.
549 Accesses
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Comparative transfection of DNA into primary and transformed mammalian cells from different lineages
Rosalie Maurisse, David De Semir, Hamid Emamekhoo, Babak Bedayat, Alireza Abdolmohammadi, Hooman Parsi, Dieter C Gruenert BMC Biotechnology 2010, 10:9 (8 February 2010)
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22.
553 Accesses
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Isolation and analysis of high quality nuclear DNA with reduced organellar DNA for plant genome sequencing and resequencing
Kerry A Lutz, Wenqin Wang, Anna Zdepski, Todd P Michael BMC Biotechnology 2011, 11:54 (20 May 2011)
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23.
642 Accesses
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Factors influencing the efficiency of generating genetically engineered pigs by nuclear transfer: multi-factorial analysis of a large data set
Mayuko Kurome, Ludwig Geistlinger, Barbara Kessler, Valeri Zakhartchenko, Nikolai Klymiuk, Annegret Wuensch, Anne Richter, Andrea Baehr, Katrin Kraehe, Katinka Burkhardt, Krzysztof Flisikowski, Tatiana Flisikowska, Claudia Merkl, Martina Landmann, Marina Durkovic, Alexander Tschukes, Simone Kraner, Dirk Schindelhauer, Tobias Petri, Alexander Kind, Hiroshi Nagashima, Angelika Schnieke, Ralf Zimmer, Eckhard Wolf BMC Biotechnology 2013, 13:43 (20 May 2013)
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24.
531 Accesses
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Nanobiotechnology can boost crop production and quality: first evidence from increased plant biomass, fruit yield and phytomedicine content in bitter melon (Momordica charantia)
Chittaranjan Kole, Phullara Kole, K Manoj Randunu, Poonam Choudhary, Ramakrishna Podila, Pu Chun Ke, Apparao M Rao, Richard K Marcus BMC Biotechnology 2013, 13:37 (26 April 2013)
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25.
545 Accesses
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Simple and efficient site-directed mutagenesis using two single-primer reactions in parallel to generate mutants for protein structure-function studies
Oded Edelheit, Aaron Hanukoglu, Israel Hanukoglu BMC Biotechnology 2009, 9:61 (30 June 2009)
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