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	1. 1253 Accesses	Methodology article A new method to customize protein expression vectors for fast, efficient and background free parallel cloning Judith Scholz, Hüseyin Besir, Claudia Strasser, Sabine Suppmann BMC Biotechnology 2013, 13:12 (14 February 2013) Abstract \| Full text \| PDF \| PubMed
	2. 1060 Accesses	Methodology article An efficient one-step site-directed deletion, insertion, single and multiple-site plasmid mutagenesis protocol Huanting Liu, James H Naismith BMC Biotechnology 2008, 8:91 (4 December 2008) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	3. 1014 Accesses	Research article Protein body formation in stable transgenic tobacco expressing elastin-like polypeptide and hydrophobin fusion proteins Sonia P Gutiérrez, Reza Saberianfar, Susanne E Kohalmi, Rima Menassa BMC Biotechnology 2013, 13:40 (10 May 2013) Abstract \| Full text \| PDF \| ePUB \| PubMed
	4. 924 Accesses	Methodology article Real-time PCR based on SYBR-Green I fluorescence: An alternative to the TaqMan assay for a relative quantification of gene rearrangements, gene amplifications and micro gene deletions Frederique Ponchel, Carmel Toomes, Kieran Bransfield, Fong T Leong, Susan H Douglas, Sarah L Field, Sandra M Bell, Valerie Combaret, Alain Puisieux, Alan J Mighell, Philip A Robinson, Chris F Inglehearn, John D Isaacs, Alex F Markham BMC Biotechnology 2003, 3:18 (13 October 2003) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	5. 876 Accesses	Research article Characterization of a novel swollenin from Penicillium oxalicum in facilitating enzymatic saccharification of cellulose Kang Kang, Shaowen Wang, Guohong Lai, Gang Liu, Miao Xing BMC Biotechnology 2013, 13:42 (20 May 2013) Abstract \| Full text \| PDF \| ePUB \| PubMed \| Cited on BioMed Central
	6. 740 Accesses	Review Less is more: strategies to remove marker genes from transgenic plants Yuan-Yeu Yau, C Neal Stewart BMC Biotechnology 2013, 13:36 (23 April 2013) Abstract \| Provisional PDF \| PubMed \| Editor’s summary Yuan-Yeu Yau and C Neal Stewart review existing strategies to remove selectable marker genes from transgenic plants, including site specific recombination systems, and explore emerging technologies for increased precision genome modification.
	7. 713 Accesses	Methodology article A high-throughput cell migration assay using scratch wound healing, a comparison of image-based readout methods Justin C Yarrow, Zachary E Perlman, Nicholas J Westwood, Timothy J Mitchison BMC Biotechnology 2004, 4:21 (9 September 2004) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central \| F1000 Biology \| Editor’s summary Adaptation of the scratch wound-healing assay to a 384 well-plate format enables a high throughput analysis of cell migration and other cell processes involved in wound healing.
	8. 707 Accesses	Methodology article FastCloning: a highly simplified, purification-free, sequence- and ligation-independent PCR cloning method Chaokun Li, Aiyun Wen, Benchang Shen, Jia Lu, Yao Huang, Yongchang Chang BMC Biotechnology 2011, 11:92 (12 October 2011) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	9. 710 Accesses	Research article Bacillus pumilus laccase: a heat stable enzyme with a wide substrate spectrum Renate Reiss, Julian Ihssen, Linda Thöny-Meyer BMC Biotechnology 2011, 11:9 (25 January 2011) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	10. 662 Accesses	Research article Strategy for successful expression of the Pseudomonas putida nitrile hydratase activator P14K in Escherichia coli Yi Liu, Wenjing Cui, Yueqin Fang, Yuechun Yu, Youtian Cui, Yuanyuan Xia, Michihiko Kobayashi, Zhemin Zhou BMC Biotechnology 2013, 13:48 (3 June 2013) Abstract \| Full text \| PDF \| ePUB \| PubMed
	11. 675 Accesses	Research article Enhanced itaconic acid production in Aspergillus niger using genetic modification and medium optimization An Li, Nina Pfelzer, Robbert Zuijderwijk, Peter Punt BMC Biotechnology 2012, 12:57 (27 August 2012) Abstract \| Full text \| PDF \| PubMed
	12. 704 Accesses	Research article Achieving pH control in microalgal cultures through fed-batch addition of stoichiometrically-balanced growth media Megerle L Scherholz, Wayne R Curtis BMC Biotechnology 2013, 13:39 (7 May 2013) Abstract \| Provisional PDF \| PubMed
	13. 716 Accesses	Research article In situ magnetic separation of antibody fragments from Escherichia coli in complex media Martin Cerff, Alexander Scholz, Matthias Franzreb, Iris L Batalha, Ana Cecilia Roque, Clemens Posten BMC Biotechnology 2013, 13:44 (20 May 2013) Abstract \| Provisional PDF \| PubMed
	14. 614 Accesses	Research article Expression and efficient secretion of a functional chitinase from Chromobacterium violaceum in Escherichia coli Marina Duarte Lobo, Fredy Davi Silva, Patrícia Gadelha Landim, Paloma Ribeiro da Cruz, Thaís Lima de Brito, Suelen Carneiro de Medeiros, José Tadeu Oliveira, Ilka Maria Vasconcelos, Humberto D¿Muniz Pereira, Thalles Barbosa Grangeiro BMC Biotechnology 2013, 13:46 (1 June 2013) Abstract \| Provisional PDF \| PubMed
	15. 625 Accesses	Research article Improved mycobacterial protein production using a Mycobacterium smegmatis groEL1ΔC expression strain Elke E Noens, Chris Williams, Madhankumar Anandhakrishnan, Christian Poulsen, Matthias T Ehebauer, Matthias Wilmanns BMC Biotechnology 2011, 11:27 (25 March 2011) Abstract \| Full text \| PDF \| PubMed
	16. 622 Accesses	Research article Optimization of culture conditions to produce high yields of active Acetobacter sp. CCTCC M209061 cells for anti-Prelog reduction of prochiral ketones Xiao-Hong Chen, Wen-Yong Lou, Min-Hua Zong, Thomas J Smith BMC Biotechnology 2011, 11:110 (20 November 2011) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	17. 590 Accesses	Research article Metabolic evolution of Corynebacterium glutamicum for increased production of L-ornithine Ling-Yan Jiang, Shang-Guang Chen, Yuan-Yuan Zhang, Jian-Zhong Liu BMC Biotechnology 2013, 13:47 (1 June 2013) Abstract \| Full text \| PDF \| PubMed
	18. 567 Accesses	Research article Towards a novel influenza vaccine: engineering of hemagglutinin on a platform of adenovirus dodecahedron Antonina Naskalska, Ewa Szolajska, Igor Andreev, Malgorzata Podsiadla, Jadwiga Chroboczek BMC Biotechnology 2013, 13:50 (16 June 2013) Abstract \| Provisional PDF
	19. 589 Accesses	Research article Generation of a tumor- and tissue-specific episomal non-viral vector system Rudolf Haase, Terese Magnusson, Baowei Su, Florian Kopp, Ernst Wagner, Hans Lipps, Armin Baiker, Manfred Ogris BMC Biotechnology 2013, 13:49 (4 June 2013) Abstract \| Provisional PDF \| PubMed
	20. 571 Accesses	Research article Optimization and validation of mitochondria-based functional assay as a useful tool to identify BH3-like molecules selectively targeting anti-apoptotic Bcl-2 proteins Jianting Long, Liu Liu, Zaneta Nikolovska-Coleska, Sanjeev Shangary, Han Yi, Shenming Wang, Shaomeng Wang BMC Biotechnology 2013, 13:45 (24 May 2013) Abstract \| Provisional PDF \| PubMed
	21. 549 Accesses	Research article Comparative transfection of DNA into primary and transformed mammalian cells from different lineages Rosalie Maurisse, David De Semir, Hamid Emamekhoo, Babak Bedayat, Alireza Abdolmohammadi, Hooman Parsi, Dieter C Gruenert BMC Biotechnology 2010, 10:9 (8 February 2010) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	22. 553 Accesses	Methodology article Isolation and analysis of high quality nuclear DNA with reduced organellar DNA for plant genome sequencing and resequencing Kerry A Lutz, Wenqin Wang, Anna Zdepski, Todd P Michael BMC Biotechnology 2011, 11:54 (20 May 2011) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	23. 642 Accesses	Research article Factors influencing the efficiency of generating genetically engineered pigs by nuclear transfer: multi-factorial analysis of a large data set Mayuko Kurome, Ludwig Geistlinger, Barbara Kessler, Valeri Zakhartchenko, Nikolai Klymiuk, Annegret Wuensch, Anne Richter, Andrea Baehr, Katrin Kraehe, Katinka Burkhardt, Krzysztof Flisikowski, Tatiana Flisikowska, Claudia Merkl, Martina Landmann, Marina Durkovic, Alexander Tschukes, Simone Kraner, Dirk Schindelhauer, Tobias Petri, Alexander Kind, Hiroshi Nagashima, Angelika Schnieke, Ralf Zimmer, Eckhard Wolf BMC Biotechnology 2013, 13:43 (20 May 2013) Abstract \| Provisional PDF \| PubMed
	24. 531 Accesses	Research article Nanobiotechnology can boost crop production and quality: first evidence from increased plant biomass, fruit yield and phytomedicine content in bitter melon (Momordica charantia) Chittaranjan Kole, Phullara Kole, K Manoj Randunu, Poonam Choudhary, Ramakrishna Podila, Pu Chun Ke, Apparao M Rao, Richard K Marcus BMC Biotechnology 2013, 13:37 (26 April 2013) Abstract \| Full text \| PDF \| ePUB \| PubMed
	25. 545 Accesses	Methodology article Simple and efficient site-directed mutagenesis using two single-primer reactions in parallel to generate mutants for protein structure-function studies Oded Edelheit, Aaron Hanukoglu, Israel Hanukoglu BMC Biotechnology 2009, 9:61 (30 June 2009) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central