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	1. 2175 Accesses	Research article GMO detection using a bioluminescent real time reporter (BART) of loop mediated isothermal amplification (LAMP) suitable for field use Guy Kiddle, Patrick Hardinge, Neil Buttigieg, Olga Gandelman, Clint Pereira, Cathal J McElgunn, Manuela Rizzoli, Rebecca Jackson, Nigel Appleton, Cathy Moore, Laurence C Tisi, James AH Murray BMC Biotechnology 2012, 12:15 (30 April 2012) Abstract \| Provisional PDF \| PubMed \| Editor’s summary Genetically modified maize DNA can be effectively detected even at low levels of contamination by the coupling of loop mediated isothermal amplification (LAMP) to a bioluminescent output produced in real-time (BART).
	2. 1175 Accesses	Research article Very bright orange fluorescent plants: endoplasmic reticulum targeting of orange fluorescent proteins as visual reporters in transgenic plants Davd GJ Mann, Laura L Abercrombie, Mary R Rudis, Reginald J Millwood, John R Dunlap, C Neal Stewart BMC Biotechnology 2012, 12:17 (3 May 2012) Abstract \| Provisional PDF \| PubMed \| Editor’s summary The tandem dimer tdTomato and the monomeric mOrange are the two highest fluorescing orange fluorescent proteins (OFPs) available as reporters for transgenic plants, when targeted to the endoplasmic reticulum.
	3. 928 Accesses	Methodology article Real-time PCR based on SYBR-Green I fluorescence: An alternative to the TaqMan assay for a relative quantification of gene rearrangements, gene amplifications and micro gene deletions Frederique Ponchel, Carmel Toomes, Kieran Bransfield, Fong T Leong, Susan H Douglas, Sarah L Field, Sandra M Bell, Valerie Combaret, Alain Puisieux, Alan J Mighell, Philip A Robinson, Chris F Inglehearn, John D Isaacs, Alex F Markham BMC Biotechnology 2003, 3:18 (13 October 2003) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	4. 798 Accesses	Methodology article New cell line development for antibody-producing Chinese hamster ovary cells using split green fluorescent protein Yeon-Gu Kim, Byoungwoo Park, Jung Oh Ahn, Joon-Ki Jung, Hong Weon Lee, Eun Gyo Lee BMC Biotechnology 2012, 12:24 (15 May 2012) Abstract \| Provisional PDF \| PubMed
	5. 797 Accesses	Research article Isolation and characterization of wharton's jellyderived multipotent mesenchymal stromal cells obtained from bovine umbilical cord and maintained in a defined serum-free three-dimensional system Tereza C Cardoso, Heitor F Ferrari, Andrea F Garcia, Juliana B Novais, Camila S Silva-Frade, Marina C Ferrarezi, Alexandre L Andrade, Roberto Gameiro BMC Biotechnology 2012, 12:18 (4 May 2012) Abstract \| Provisional PDF \| PubMed
	6. 793 Accesses	Methodology article An efficient one-step site-directed deletion, insertion, single and multiple-site plasmid mutagenesis protocol Huanting Liu, James H Naismith BMC Biotechnology 2008, 8:91 (4 December 2008) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	7. 628 Accesses	Methodology article Simple and efficient site-directed mutagenesis using two single-primer reactions in parallel to generate mutants for protein structure-function studies Oded Edelheit, Aaron Hanukoglu, Israel Hanukoglu BMC Biotechnology 2009, 9:61 (30 June 2009) Abstract \| Full text \| PDF \| PubMed
	8. 626 Accesses	Methodology article Solution-based targeted genomic enrichment for precious DNA samples Aiden E Shearer, Michael S Hildebrand, Richard JH Smith BMC Biotechnology 2012, 12:20 (4 May 2012) Abstract \| Provisional PDF \| PubMed
	9. 608 Accesses	Research article RNA viral vectors for improved Agrobacterium-mediated transient expression of heterologous proteins in Nicotiana benthamiana cell suspensions and hairy roots Jeffrey S Larsen, Wayne R Curtis BMC Biotechnology 2012, 12:21 (6 May 2012) Abstract \| Provisional PDF \| PubMed
	10. 591 Accesses	Research article Pseudomonas fluorescens biofilms subjected to phage phiIBB-PF7A Sanna Sillankorva, Peter Neubauer, Joana Azeredo BMC Biotechnology 2008, 8:79 (27 October 2008) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	11. 561 Accesses	Research article Validation of glypican-3-specific scFv isolated from paired display/secretory yeast display library Yonghai Li, Donald L Siegel, Nathalie Scholler, David E Kaplan BMC Biotechnology 2012, 12:23 (7 May 2012) Abstract \| Provisional PDF \| PubMed
	12. 552 Accesses	Research article Oil accumulation in the model green alga Chlamydomonas reinhardtii: characterization, variability between common laboratory strains and relationship with starch reserves Magali Siaut, Stéphan Cuiné, Caroline Cagnon, Boris Fessler, Mai Nguyen, Patrick Carrier, Audrey Beyly, Fred Beisson, Christian Triantaphylidès, Yonghua Li-Beisson, Gilles Peltier BMC Biotechnology 2011, 11:7 (21 January 2011) Abstract \| Full text \| PDF \| PubMed
	13. 544 Accesses	Research article Setting up a platform for plant-based influenza virus vaccine production in South Africa Elizabeth Mortimer, James M Maclean, Sandiswa Mbewana, Amelia Buys, Anna-Lise Williamson, Inga I Hitzeroth, Edward P Rybicki BMC Biotechnology 2012, 12:14 (26 April 2012) Abstract \| Full text \| PDF \| PubMed
	14. 485 Accesses	Research article Untranslated regions of diverse plant viral RNAs vary greatly in translation enhancement efficiency Qiuling Fan, Krzysztof Treder, W. Allen Miller BMC Biotechnology 2012, 12:22 (6 May 2012) Abstract \| Provisional PDF \| PubMed
	15. 466 Accesses	Methodology article A simple technique for quantifying apoptosis in 96-well plates Deborah Ribble, Nathaniel B Goldstein, David A Norris, Yiqun G Shellman BMC Biotechnology 2005, 5:12 (10 May 2005) Abstract \| Full text \| PDF \| PubMed
	16. 457 Accesses	Methodology article Pattern analysis approach reveals restriction enzyme cutting abnormalities and other cDNA library construction artifacts using raw EST data Sun Zhou, Guoli Ji, Xiaolin Liu, Pei Li, James Moler, John E Karro, Chun Liang BMC Biotechnology 2012, 12:16 (3 May 2012) Abstract \| Provisional PDF \| PubMed
	17. 455 Accesses	Research article Characterization of mercury bioremediation by transgenic bacteria expressing metallothionein and polyphosphate kinase Oscar N Ruiz, Derry Alvarez, Gloriene Gonzalez-Ruiz, Cesar Torres BMC Biotechnology 2011, 11:82 (12 August 2011) Abstract \| Full text \| PDF \| PubMed \| Editor’s summary Engineered bacteria expressing the heavy metal scavenging molecules metallothionein or polyphosphate kinase are resistant to mercury and can detoxify their environment by accumulating most of the toxic element, which could be subsequently recycled for further industrial applications.
	18. 449 Accesses	Methodology article FastCloning: a highly simplified, purification-free, sequence- and ligation-independent PCR cloning method Chaokun Li, Aiyun Wen, Benchang Shen, Jia Lu, Yao Huang, Yongchang Chang BMC Biotechnology 2011, 11:92 (12 October 2011) Abstract \| Full text \| PDF \| PubMed
	19. 444 Accesses	Methodology article A high-throughput cell migration assay using scratch wound healing, a comparison of image-based readout methods Justin C Yarrow, Zachary E Perlman, Nicholas J Westwood, Timothy J Mitchison BMC Biotechnology 2004, 4:21 (9 September 2004) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central \| F1000 Biology \| Editor’s summary Adaptation of the scratch wound-healing assay to a 384 well-plate format enables a high throughput analysis of cell migration and other cell processes involved in wound healing.
	20. 437 Accesses	Research article A compact phage display human scFv library for selection of antibodies to a wide variety of antigens Potjamas Pansri, Nanthnit Jaruseranee, Kuntalee Rangnoi, Peter Kristensen, Montarop Yamabhai BMC Biotechnology 2009, 9:6 (29 January 2009) Abstract \| Full text \| PDF \| PubMed
	21. 430 Accesses	Methodology article Creation and validation of a ligation-independent cloning (LIC) retroviral vector for stable gene transduction in mammalian cells Asmita Patel, Anisleidys Muñoz, Katherine Halvorsen, Priyamvada Rai BMC Biotechnology 2012, 12:3 (16 January 2012) Abstract \| Full text \| PDF \| PubMed
	22. 405 Accesses	Methodology article Isolation and analysis of high quality nuclear DNA with reduced organellar DNA for plant genome sequencing and resequencing Kerry A Lutz, Wenqin Wang, Anna Zdepski, Todd P Michael BMC Biotechnology 2011, 11:54 (20 May 2011) Abstract \| Full text \| PDF \| PubMed
	23. 385 Accesses	Methodology article Rapid isolation of yeast genomic DNA: Bust n' Grab Susanna Harju, Halyna Fedosyuk, Kenneth R Peterson BMC Biotechnology 2004, 4:8 (21 April 2004) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	24. 368 Accesses	Research article Comparative transfection of DNA into primary and transformed mammalian cells from different lineages Rosalie Maurisse, David De Semir, Hamid Emamekhoo, Babak Bedayat, Alireza Abdolmohammadi, Hooman Parsi, Dieter C Gruenert BMC Biotechnology 2010, 10:9 (8 February 2010) Abstract \| Full text \| PDF \| PubMed
	25. 354 Accesses	Research article Development of a diagnostic method for neosporosis in cattle using recombinant Neospora caninum proteins Jinhua Dong, Takahiro Otsuki, Tatsuya Kato, Enoch Y Park BMC Biotechnology 2012, 12:19 (4 May 2012) Abstract \| Provisional PDF \| PubMed