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1.

1823
Accesses

Research article   Open Access Highly Accessed

Generation and functional characterization of the anti-transferrin receptor single-chain antibody-GAL4 (TfRscFv-GAL4) fusion protein

Qing Ye, Heyu Hu, Zhihua Wang, Tong Lu, Zhiquan Hu, Xing Zeng, Shu Zhang, Jing Liu, Ping Lei, Cong-Yi Wang, Zhangqun Ye, Guanxin Shen BMC Biotechnology 2012, 12:91 (28 November 2012)

Abstract | Full text | PDF | PubMed

2.

1614
Accesses

Research article   Open Access Highly Accessed

Domain-swapping of mesophilic xylanase with hyper-thermophilic glucanase

Liangwei Liu, Linmin Wang, Zhang Zhang, Xiaodan Guo, Xiangqian Li, Hongge Chen BMC Biotechnology 2012, 12:28 (7 June 2012)

Abstract | Full text | PDF | PubMed

3.

1421
Accesses

Research article   Open Access Highly Accessed

Isolation and characterization of Wharton’s jelly-derived multipotent mesenchymal stromal cells obtained from bovine umbilical cord and maintained in a defined serum-free three-dimensional system

Tereza C Cardoso, Heitor F Ferrari, Andrea F Garcia, Juliana B Novais, Camila Silva-Frade, Marina C Ferrarezi, Alexandre L Andrade, Roberto Gameiro BMC Biotechnology 2012, 12:18 (4 May 2012)

Abstract | Full text | PDF | PubMed

4.

1422
Accesses

Research article   Open Access Highly Accessed

Glycoside Hydrolases from a targeted Compost Metagenome, activity-screening and functional characterization

Michael J Dougherty, Patrik D’haeseleer, Terry C Hazen, Blake A Simmons, Paul D Adams, Masood Z Hadi BMC Biotechnology 2012, 12:38 (3 July 2012)

Abstract | Full text | PDF | PubMed

5.

1405
Accesses

Research article   Open Access

Internalization of novel non-viral vector TAT-streptavidin into human cells

Johanna Rinne, Brian Albarran, Juulia Jylhävä, Teemu O Ihalainen, Pasi Kankaanpää, Vesa P Hytönen, Patrick S Stayton, Markku S Kulomaa, Maija Vihinen-Ranta BMC Biotechnology 2007, 7:1 (2 January 2007)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

6.

1297
Accesses

Research article   Open Access

Self-assembling Fmoc dipeptide hydrogel for in situ 3D cell culturing

Thomas Liebmann, Susanna Rydholm, Victor Akpe, Hjalmar Brismar BMC Biotechnology 2007, 7:88 (10 December 2007)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

7.

987
Accesses

Research article   Open Access Highly Accessed

Cloning, overexpression, purification, and characterization of a polyextremophilic β-galactosidase from the Antarctic haloarchaeon Halorubrum lacusprofundi

Ram Karan, Melinda D Capes, Priya DasSarma, Shiladitya DasSarma BMC Biotechnology 2013, 13:3 (16 January 2013)

Abstract | Full text | PDF | PubMed

8.

860
Accesses

Research article   Open Access

Genetic engineering of Pyrococcus furiosus to use chitin as a carbon source

Martina Kreuzer, Karolin Schmutzler, Ingrid Waege, Michael Thomm, Winfried Hausner BMC Biotechnology 2013, 13:9 (7 February 2013)

Abstract | Full text | PDF | PubMed

9.

833
Accesses

Methodology article   Open Access Highly Accessed

An efficient one-step site-directed deletion, insertion, single and multiple-site plasmid mutagenesis protocol

Huanting Liu, James H Naismith BMC Biotechnology 2008, 8:91 (4 December 2008)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

10.

767
Accesses

Research article   Open Access Highly Accessed

Facile, high efficiency immobilization of lipase enzyme on magnetic iron oxide nanoparticles via a biomimetic coating

Yuhong Ren, Jose G Rivera, Lihong He, Harsha Kulkarni, Dong-Keun Lee, Phillip B Messersmith BMC Biotechnology 2011, 11:63 (8 June 2011)

Abstract | Full text | PDF | PubMed |  Editor’s summary

Lipase can be immobilized efficiently under aqueous conditions by coating magnetic nanoparticles with a polydopamine biomimetic inspired by mussel adhesive proteins, with the enzyme still retaining high activity even after many cycles of magnetic separation and reuse.

11.

760
Accesses

Research article   Open Access

A 28 nt long synthetic 5′UTR (synJ) as an enhancer of transgene expression in dicotyledonous plants

Shaveta Kanoria, Pradeep Kumar Burma BMC Biotechnology 2012, 12:85 (10 November 2012)

Abstract | Full text | PDF | PubMed

12.

733
Accesses

Methodology article   Open Access Highly Accessed

A high-throughput cell migration assay using scratch wound healing, a comparison of image-based readout methods

Justin C Yarrow, Zachary E Perlman, Nicholas J Westwood, Timothy J Mitchison BMC Biotechnology 2004, 4:21 (9 September 2004)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central | F1000 Biology |  Editor’s summary

Adaptation of the scratch wound-healing assay to a 384 well-plate format enables a high throughput analysis of cell migration and other cell processes involved in wound healing.

13.

708
Accesses

Research article   Open Access Highly Accessed

Characterization of a newly identified rice chitinase-like protein (OsCLP) homologous to xylanase inhibitor

Jingni Wu, Yiming Wang, Sun Tae Kim, Sang Gon Kim, Kyu Young Kang BMC Biotechnology 2013, 13:4 (18 January 2013)

Abstract | Full text | PDF | PubMed

14.

705
Accesses

Methodology article   Open Access

A new method to customize protein expression vectors for fast, efficient and background free parallel cloning

Judith Scholz, Hüseyin Besir, Claudia Strasser, Sabine Suppmann BMC Biotechnology 2013, 13:12 (14 February 2013)

Abstract | Provisional PDF | PubMed

15.

699
Accesses

Methodology article   Open Access Highly Accessed

An asymmetric PCR-based, reliable and rapid single-tube native DNA engineering strategy

Yanzhen Bi, Xianfeng Qiao, Zaidong Hua, Liping Zhang, Ximei Liu, Li Li, Wenjun Hua, Hongwei Xiao, Jingrong Zhou, Qingxin Wei, Xinmin Zheng BMC Biotechnology 2012, 12:39 (6 July 2012)

Abstract | Full text | PDF | PubMed

16.

686
Accesses

Methodology article   Open Access

Graded or threshold response of the tet-controlled gene expression: all depends on the concentration of the transactivator

Niels Heinz, Katharina Hennig, Rainer Loew BMC Biotechnology 2013, 13:5 (22 January 2013)

Abstract | Full text | PDF | PubMed

17.

684
Accesses

Methodology article   Open Access Highly Accessed

Real-time PCR based on SYBR-Green I fluorescence: An alternative to the TaqMan assay for a relative quantification of gene rearrangements, gene amplifications and micro gene deletions

Frederique Ponchel, Carmel Toomes, Kieran Bransfield, Fong T Leong, Susan H Douglas, Sarah L Field, Sandra M Bell, Valerie Combaret, Alain Puisieux, Alan J Mighell, Philip A Robinson, Chris F Inglehearn, John D Isaacs, Alex F Markham BMC Biotechnology 2003, 3:18 (13 October 2003)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

18.

666
Accesses

Research article   Open Access

Dynamic strain scanning optimization: an efficient strain design strategy for balanced yield, titer, and productivity. DySScO strategy for strain design

Kai Zhuang, Laurence Yang, William R Cluett, Radhakrishnan Mahadevan BMC Biotechnology 2013, 13:8 (6 February 2013)

Abstract | Full text | PDF | PubMed

19.

640
Accesses

Research article   Open Access Highly Accessed

Layer-shaped alginate hydrogels enhance the biological performance of human adipose-derived stem cells

Bianca Galateanu, Doina Dimonie, Eugeniu Vasile, Sorin Nae, Anisoara Cimpean, Marieta Costache BMC Biotechnology 2012, 12:35 (29 June 2012)

Abstract | Full text | PDF | PubMed

20.

639
Accesses

Methodology article   Open Access

Stable nuclear transformation of Eudorina elegans

Kai Lerche, Armin Hallmann BMC Biotechnology 2013, 13:11 (12 February 2013)

Abstract | Full text | PDF | PubMed

21.

572
Accesses

Research article   Open Access

Fibres from flax overproducing beta-1,3-glucanase show increased accumulation of pectin and phenolics and thus higher antioxidant capacity

Wioleta Wojtasik, Anna Kulma, Lucyna Dymi¿ska, Jerzy Hanuza, Jacek ¿ebrowski, Jan Szopa BMC Biotechnology 2013, 13:10 (9 February 2013)

Abstract | Provisional PDF | PubMed

22.

559
Accesses

Methodology article   Open Access Highly Accessed

Simple and efficient site-directed mutagenesis using two single-primer reactions in parallel to generate mutants for protein structure-function studies

Oded Edelheit, Aaron Hanukoglu, Israel Hanukoglu BMC Biotechnology 2009, 9:61 (30 June 2009)

Abstract | Full text | PDF | PubMed

23.

560
Accesses

Research article   Open Access Highly Accessed

Oil accumulation in the model green alga Chlamydomonas reinhardtii: characterization, variability between common laboratory strains and relationship with starch reserves

Magali Siaut, Stéphan Cuiné, Caroline Cagnon, Boris Fessler, Mai Nguyen, Patrick Carrier, Audrey Beyly, Fred Beisson, Christian Triantaphylidès, Yonghua Li-Beisson, Gilles Peltier BMC Biotechnology 2011, 11:7 (21 January 2011)

Abstract | Full text | PDF | PubMed

24.

550
Accesses

Methodology article   Open Access Highly Accessed

A comparison between two brine shrimp assays to detect in vitro cytotoxicity in marine natural products

José Carballo, Zaira L Hernández-Inda, Pilar Pérez, María D García-Grávalos BMC Biotechnology 2002, 2:17 (23 September 2002)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

25.

543
Accesses

Research article   Open Access

Development of a versatile TaqManTM real-time quantitative PCR (RT-qPCR) compliant anchor sequence to quantify bacterial gene transcripts from RNA samples containing carryover genomic DNA

Vijay J Gadkar, Martin Filion BMC Biotechnology 2013, 13:7 (31 January 2013)

Abstract | Provisional PDF | PubMed

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