BMC Biotechnology | Most Viewed

Submit a manuscript Register Contact us Follow us on Twitter

Explore BMC Biotechnology

Advertisement

Articles

All articles RSS
Sections
Most popular RSS
- Most viewed
- Most forwarded
Archive
Supplements

Display/download options Articles per page: 25 | 50 | 100

Display options

Editor's summary Citation

Download options

Send
To

	All
	1. 2552 Accesses	Research article Optimization of culture conditions to produce high yields of active Acetobacter sp. CCTCC M209061 cells for anti-Prelog reduction of prochiral ketones Xiao-Hong Chen, Wen-Yong Lou, Min-Hua Zong, Thomas J Smith BMC Biotechnology 2011, 11:110 (20 November 2011) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	2. 1955 Accesses	Research article Expression and efficient secretion of a functional chitinase from Chromobacterium violaceum in Escherichia coli Marina Duarte Lobo, Fredy Davi Silva, Patrícia Gadelha Landim, Paloma da Cruz, Thaís de Brito, Suelen de Medeiros, José Tadeu Oliveira, Ilka Vasconcelos, Humberto Pereira, Thalles Grangeiro BMC Biotechnology 2013, 13:46 (1 June 2013) Abstract \| Full text \| PDF \| ePUB \| PubMed
	3. 1953 Accesses	Research article Development of efficient catharanthus roseus regeneration and transformation system using agrobacterium tumefaciens and hypocotyls as explants Quan Wang, Shihai Xing, Qifang Pan, Fang Yuan, Jingya Zhao, Yuesheng Tian, Yu Chen, Guofeng Wang, Kexuan Tang BMC Biotechnology 2012, 12:34 (29 June 2012) Abstract \| Full text \| PDF \| PubMed
	4. 1764 Accesses	Research article Sensitivity of a real-time PCR method for the detection of transgenes in a mixture of transgenic and non-transgenic seeds of papaya (Carica papaya L.) Madhugiri Nageswara-Rao, Charles Kwit, Sujata Agarwal, Mariah T Patton, Jordan A Skeen, Joshua S Yuan, Richard M Manshardt, C Stewart BMC Biotechnology 2013, 13:69 (1 September 2013) Abstract \| Full text \| PDF \| PubMed
	5. 1456 Accesses	Research article Bacillus pumilus laccase: a heat stable enzyme with a wide substrate spectrum Renate Reiss, Julian Ihssen, Linda Thöny-Meyer BMC Biotechnology 2011, 11:9 (25 January 2011) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	6. 1304 Accesses	Research article Expression of recombinant staphylokinase in the methylotrophic yeast Hansenula polymorpha Manal Moussa, Mahmoud Ibrahim, Maria El Ghazaly, Jan Rohde, Stefan Gnoth, Andreas Anton, Frank Kensy, Frank Mueller BMC Biotechnology 2012, 12:96 (19 December 2012) Abstract \| Full text \| PDF \| PubMed
	7. 1200 Accesses	Methodology article Assaying proline hydroxylation in recombinant collagen variants by liquid chromatography-mass spectrometry S W Chan, John Greaves, Nancy A Da Silva, Szu-Wen Wang BMC Biotechnology 2012, 12:51 (17 August 2012) Abstract \| Full text \| PDF \| PubMed \| Editor’s summary A novel liquid chromatography-mass spectrometry method evaluates proline hydroxylation in recombinant collagen, allowing accurate determination of hydroxylation in yeast expression systems and represents a useful alternative to conventional amino acid analysis.
	8. 1198 Accesses	Methodology article An efficient one-step site-directed deletion, insertion, single and multiple-site plasmid mutagenesis protocol Huanting Liu, James H Naismith BMC Biotechnology 2008, 8:91 (4 December 2008) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	9. 1187 Accesses	Review Less is more: strategies to remove marker genes from transgenic plants Yuan-Yeu Yau, C Neal Stewart BMC Biotechnology 2013, 13:36 (23 April 2013) Abstract \| Full text \| PDF \| ePUB \| PubMed \| Editor’s summary Yuan-Yeu Yau and C Neal Stewart review existing strategies to remove selectable marker genes from transgenic plants, including site specific recombination systems, and explore emerging technologies for increased precision genome modification.
	10. 1184 Accesses	Research article Tender coconut water an economical growth medium for the production of recombinant proteins in Escherichia coli Narendrakumar Sekar, Soumya Veetil, Muniasamy Neerathilingam BMC Biotechnology 2013, 13:70 (2 September 2013) Abstract \| Full text \| PDF \| PubMed
	11. 1135 Accesses	Research article Cloning and characterization of a novel 2-ketoisovalerate reductase from the beauvericin producer Fusarium proliferatum LF061 Tao Zhang, XiaoPeng Jia, Ying Zhuo, Mei Liu, Hong Gao, JinTao Liu, Lixin Zhang BMC Biotechnology 2012, 12:55 (23 August 2012) Abstract \| Full text \| PDF \| PubMed
	12. 1100 Accesses	Research article Monoclonal antibody humanness score and its applications Sean H Gao, Kexin Huang, Hua Tu, Adam S Adler BMC Biotechnology 2013, 13:55 (5 July 2013) Abstract \| Full text \| PDF \| ePUB \| PubMed
	13. 1021 Accesses	Research article Identification of an endo-1,4-beta-xylanase of Ustilago maydis Elena Geiser, Nick Wierckx, Martin Zimmermann, Lars M Blank BMC Biotechnology 2013, 13:59 (26 July 2013) Abstract \| Full text \| PDF \| ePUB \| PubMed
	14. 1002 Accesses	Research article Genetic load and transgenic mitigating genes in transgenic Brassica rapa (field mustard) × Brassica napus (oilseed rape) hybrid populations Christy W Rose, Reginald J Millwood, Hong S Moon, Murali R Rao, Matthew D Halfhill, Paul L Raymer, Suzanne I Warwick, Hani Al-Ahmad, Jonathan Gressel, C Neal Stewart BMC Biotechnology 2009, 9:93 (31 October 2009) Abstract \| Full text \| PDF \| PubMed \| Editor’s summary Brassica rapa (weed) x Brassica napus (crop) transgenic hybrids are less fit than B. rapa in competitive conditions, with crop alleles acting as a negative genetic load opposing transgene introgression, while a mitigating transgene is soon lost from hybrids.
	15. 881 Accesses	Research article Optimisation of the foot-and-mouth disease virus 2A co-expression system for biomedical applications Ekaterina Minskaia, John Nicholson, Martin D Ryan BMC Biotechnology 2013, 13:67 (22 August 2013) Abstract \| Full text \| PDF \| ePUB \| PubMed
	16. 854 Accesses	Research article Automated serial extraction of DNA and RNA from biobanked tissue specimens Lucy Mathot, Monica Wallin, Tobias Sjöblom BMC Biotechnology 2013, 13:66 (19 August 2013) Abstract \| Full text \| PDF \| ePUB \| PubMed
	17. 812 Accesses	Research article Systematic screening of glycosylation- and trafficking-associated gene knockouts in Saccharomyces cerevisiae identifies mutants with improved heterologous exocellulase activity and host secretion Tzi-Yuan Wang, Chih-Jen Huang, Hsin-Liang Chen, Po-Chun Ho, Huei-Mien Ke, Hsing-Yi Cho, Sz-Kai Ruan, Kuo-Yen Hung, I-Li Wang, Ya-Wun Cai, Huang-Mo Sung, Wen-Hsiung Li, Ming-Che Shih BMC Biotechnology 2013, 13:71 (3 September 2013) Abstract \| Full text \| PDF \| ePUB \| PubMed
	18. 800 Accesses	Research article Cloning, sequence analysis, expression of Cyathus bulleri laccase in Pichia pastoris and characterization of recombinant laccase Neha Garg, Nora Bieler, Tenzin Kenzom, Meenu Chhabra, Marion Ansorge-Schumacher, Saroj Mishra BMC Biotechnology 2012, 12:75 (23 October 2012) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	19. 798 Accesses	Methodology article Real-time PCR based on SYBR-Green I fluorescence: An alternative to the TaqMan assay for a relative quantification of gene rearrangements, gene amplifications and micro gene deletions Frederique Ponchel, Carmel Toomes, Kieran Bransfield, Fong T Leong, Susan H Douglas, Sarah L Field, Sandra M Bell, Valerie Combaret, Alain Puisieux, Alan J Mighell, Philip A Robinson, Chris F Inglehearn, John D Isaacs, Alex F Markham BMC Biotechnology 2003, 3:18 (13 October 2003) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	20. 770 Accesses	Methodology article A high-throughput cell migration assay using scratch wound healing, a comparison of image-based readout methods Justin C Yarrow, Zachary E Perlman, Nicholas J Westwood, Timothy J Mitchison BMC Biotechnology 2004, 4:21 (9 September 2004) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central \| f1000 \| Editor’s summary Adaptation of the scratch wound-healing assay to a 384 well-plate format enables a high throughput analysis of cell migration and other cell processes involved in wound healing.
	21. 768 Accesses	Methodology article Sequence specific visual detection of LAMP reactions by addition of cationic polymers Yasuyoshi Mori, Tsuyoshi Hirano, Tsugunori Notomi BMC Biotechnology 2006, 6:3 (10 January 2006) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	22. 760 Accesses	Research article Design and characterization of genetically engineered zebrafish aquaporin-3 mutants highly permeable to the cryoprotectant ethylene glycol François Chauvigné, Esther Lubzens, Joan Cerdà BMC Biotechnology 2011, 11:34 (8 April 2011) Abstract \| Full text \| PDF \| PubMed
	23. 758 Accesses	Research article T4 RNA Ligase 2 truncated active site mutants: improved tools for RNA analysis Sebastien Viollet, Ryan T Fuchs, Daniela B Munafo, Fanglei Zhuang, Gregory B Robb BMC Biotechnology 2011, 11:72 (1 July 2011) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	24. 750 Accesses	Research article Decolorization of a recalcitrant organic compound (Melanoidin) by a novel thermotolerant yeast, Candida tropicalis RG-9 Soni Tiwari, Rajeeva Gaur, Ranjan Singh BMC Biotechnology 2012, 12:30 (18 June 2012) Abstract \| Full text \| PDF \| PubMed
	25. 749 Accesses	Research article Stable high volumetric production of glycosylated human recombinant IFNalpha2b in HEK293 cells Martin Loignon, Sylvie Perret, John Kelly, Denise Boulais, Brian Cass, Louis Bisson, Fatemeh Afkhamizarreh, Yves Durocher BMC Biotechnology 2008, 8:65 (27 August 2008) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central