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1.

2552
Accesses

Research article   Open Access Highly Accessed

Optimization of culture conditions to produce high yields of active Acetobacter sp. CCTCC M209061 cells for anti-Prelog reduction of prochiral ketones

Xiao-Hong Chen, Wen-Yong Lou, Min-Hua Zong, Thomas J Smith BMC Biotechnology 2011, 11:110 (20 November 2011)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

2.

1955
Accesses

Research article   Open Access Highly Accessed

Expression and efficient secretion of a functional chitinase from Chromobacterium violaceum in Escherichia coli

Marina Duarte Lobo, Fredy Davi Silva, Patrícia Gadelha Landim, Paloma da Cruz, Thaís de Brito, Suelen de Medeiros, José Tadeu Oliveira, Ilka Vasconcelos, Humberto Pereira, Thalles Grangeiro BMC Biotechnology 2013, 13:46 (1 June 2013)

Abstract | Full text | PDF | ePUB | PubMed

3.

1953
Accesses

Research article   Open Access Highly Accessed

Development of efficient catharanthus roseus regeneration and transformation system using agrobacterium tumefaciens and hypocotyls as explants

Quan Wang, Shihai Xing, Qifang Pan, Fang Yuan, Jingya Zhao, Yuesheng Tian, Yu Chen, Guofeng Wang, Kexuan Tang BMC Biotechnology 2012, 12:34 (29 June 2012)

Abstract | Full text | PDF | PubMed

4.

1764
Accesses

Research article   Open Access Highly Accessed

Sensitivity of a real-time PCR method for the detection of transgenes in a mixture of transgenic and non-transgenic seeds of papaya (Carica papaya L.)

Madhugiri Nageswara-Rao, Charles Kwit, Sujata Agarwal, Mariah T Patton, Jordan A Skeen, Joshua S Yuan, Richard M Manshardt, C Stewart BMC Biotechnology 2013, 13:69 (1 September 2013)

Abstract | Full text | PDF | PubMed

5.

1456
Accesses

Research article   Open Access Highly Accessed

Bacillus pumilus laccase: a heat stable enzyme with a wide substrate spectrum

Renate Reiss, Julian Ihssen, Linda Thöny-Meyer BMC Biotechnology 2011, 11:9 (25 January 2011)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

6.

1304
Accesses

Research article   Open Access Highly Accessed

Expression of recombinant staphylokinase in the methylotrophic yeast Hansenula polymorpha

Manal Moussa, Mahmoud Ibrahim, Maria El Ghazaly, Jan Rohde, Stefan Gnoth, Andreas Anton, Frank Kensy, Frank Mueller BMC Biotechnology 2012, 12:96 (19 December 2012)

Abstract | Full text | PDF | PubMed

7.

1200
Accesses

Methodology article   Open Access Highly Accessed

Assaying proline hydroxylation in recombinant collagen variants by liquid chromatography-mass spectrometry

S W Chan, John Greaves, Nancy A Da Silva, Szu-Wen Wang BMC Biotechnology 2012, 12:51 (17 August 2012)

Abstract | Full text | PDF | PubMed |  Editor’s summary

A novel liquid chromatography-mass spectrometry method evaluates proline hydroxylation in recombinant collagen, allowing accurate determination of hydroxylation in yeast expression systems and represents a useful alternative to conventional amino acid analysis.

8.

1198
Accesses

Methodology article   Open Access Highly Accessed

An efficient one-step site-directed deletion, insertion, single and multiple-site plasmid mutagenesis protocol

Huanting Liu, James H Naismith BMC Biotechnology 2008, 8:91 (4 December 2008)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

9.

1187
Accesses

Review   Open Access Highly Accessed

Less is more: strategies to remove marker genes from transgenic plants

Yuan-Yeu Yau, C Neal Stewart BMC Biotechnology 2013, 13:36 (23 April 2013)

Abstract | Full text | PDF | ePUB | PubMed |  Editor’s summary

Yuan-Yeu Yau and C Neal Stewart review existing strategies to remove selectable marker genes from transgenic plants, including site specific recombination systems, and explore emerging technologies for increased precision genome modification.

10.

1184
Accesses

Research article   Open Access Highly Accessed

Tender coconut water an economical growth medium for the production of recombinant proteins in Escherichia coli

Narendrakumar Sekar, Soumya Veetil, Muniasamy Neerathilingam BMC Biotechnology 2013, 13:70 (2 September 2013)

Abstract | Full text | PDF | PubMed

11.

1135
Accesses

Research article   Open Access

Cloning and characterization of a novel 2-ketoisovalerate reductase from the beauvericin producer Fusarium proliferatum LF061

Tao Zhang, XiaoPeng Jia, Ying Zhuo, Mei Liu, Hong Gao, JinTao Liu, Lixin Zhang BMC Biotechnology 2012, 12:55 (23 August 2012)

Abstract | Full text | PDF | PubMed

12.

1100
Accesses

Research article   Open Access Highly Accessed

Monoclonal antibody humanness score and its applications

Sean H Gao, Kexin Huang, Hua Tu, Adam S Adler BMC Biotechnology 2013, 13:55 (5 July 2013)

Abstract | Full text | PDF | ePUB | PubMed

13.

1021
Accesses

Research article   Open Access

Identification of an endo-1,4-beta-xylanase of Ustilago maydis

Elena Geiser, Nick Wierckx, Martin Zimmermann, Lars M Blank BMC Biotechnology 2013, 13:59 (26 July 2013)

Abstract | Full text | PDF | ePUB | PubMed

14.

1002
Accesses

Research article   Open Access

Genetic load and transgenic mitigating genes in transgenic Brassica rapa (field mustard) × Brassica napus (oilseed rape) hybrid populations

Christy W Rose, Reginald J Millwood, Hong S Moon, Murali R Rao, Matthew D Halfhill, Paul L Raymer, Suzanne I Warwick, Hani Al-Ahmad, Jonathan Gressel, C Neal Stewart BMC Biotechnology 2009, 9:93 (31 October 2009)

Abstract | Full text | PDF | PubMed |  Editor’s summary

Brassica rapa (weed) x Brassica napus (crop) transgenic hybrids are less fit than B. rapa in competitive conditions, with crop alleles acting as a negative genetic load opposing transgene introgression, while a mitigating transgene is soon lost from hybrids.

15.

881
Accesses

Research article   Open Access

Optimisation of the foot-and-mouth disease virus 2A co-expression system for biomedical applications

Ekaterina Minskaia, John Nicholson, Martin D Ryan BMC Biotechnology 2013, 13:67 (22 August 2013)

Abstract | Full text | PDF | ePUB | PubMed

16.

854
Accesses

Research article   Open Access Highly Accessed

Automated serial extraction of DNA and RNA from biobanked tissue specimens

Lucy Mathot, Monica Wallin, Tobias Sjöblom BMC Biotechnology 2013, 13:66 (19 August 2013)

Abstract | Full text | PDF | ePUB | PubMed

17.

812
Accesses

Research article   Open Access

Systematic screening of glycosylation- and trafficking-associated gene knockouts in Saccharomyces cerevisiae identifies mutants with improved heterologous exocellulase activity and host secretion

Tzi-Yuan Wang, Chih-Jen Huang, Hsin-Liang Chen, Po-Chun Ho, Huei-Mien Ke, Hsing-Yi Cho, Sz-Kai Ruan, Kuo-Yen Hung, I-Li Wang, Ya-Wun Cai, Huang-Mo Sung, Wen-Hsiung Li, Ming-Che Shih BMC Biotechnology 2013, 13:71 (3 September 2013)

Abstract | Full text | PDF | ePUB | PubMed

18.

800
Accesses

Research article   Open Access

Cloning, sequence analysis, expression of Cyathus bulleri laccase in Pichia pastoris and characterization of recombinant laccase

Neha Garg, Nora Bieler, Tenzin Kenzom, Meenu Chhabra, Marion Ansorge-Schumacher, Saroj Mishra BMC Biotechnology 2012, 12:75 (23 October 2012)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

19.

798
Accesses

Methodology article   Open Access Highly Accessed

Real-time PCR based on SYBR-Green I fluorescence: An alternative to the TaqMan assay for a relative quantification of gene rearrangements, gene amplifications and micro gene deletions

Frederique Ponchel, Carmel Toomes, Kieran Bransfield, Fong T Leong, Susan H Douglas, Sarah L Field, Sandra M Bell, Valerie Combaret, Alain Puisieux, Alan J Mighell, Philip A Robinson, Chris F Inglehearn, John D Isaacs, Alex F Markham BMC Biotechnology 2003, 3:18 (13 October 2003)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

20.

770
Accesses

Methodology article   Open Access Highly Accessed

A high-throughput cell migration assay using scratch wound healing, a comparison of image-based readout methods

Justin C Yarrow, Zachary E Perlman, Nicholas J Westwood, Timothy J Mitchison BMC Biotechnology 2004, 4:21 (9 September 2004)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central | f1000 |  Editor’s summary

Adaptation of the scratch wound-healing assay to a 384 well-plate format enables a high throughput analysis of cell migration and other cell processes involved in wound healing.

21.

768
Accesses

Methodology article   Open Access

Sequence specific visual detection of LAMP reactions by addition of cationic polymers

Yasuyoshi Mori, Tsuyoshi Hirano, Tsugunori Notomi BMC Biotechnology 2006, 6:3 (10 January 2006)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

22.

760
Accesses

Research article   Open Access

Design and characterization of genetically engineered zebrafish aquaporin-3 mutants highly permeable to the cryoprotectant ethylene glycol

François Chauvigné, Esther Lubzens, Joan Cerdà BMC Biotechnology 2011, 11:34 (8 April 2011)

Abstract | Full text | PDF | PubMed

23.

758
Accesses

Research article   Open Access

T4 RNA Ligase 2 truncated active site mutants: improved tools for RNA analysis

Sebastien Viollet, Ryan T Fuchs, Daniela B Munafo, Fanglei Zhuang, Gregory B Robb BMC Biotechnology 2011, 11:72 (1 July 2011)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

24.

750
Accesses

Research article   Open Access Highly Accessed

Decolorization of a recalcitrant organic compound (Melanoidin) by a novel thermotolerant yeast, Candida tropicalis RG-9

Soni Tiwari, Rajeeva Gaur, Ranjan Singh BMC Biotechnology 2012, 12:30 (18 June 2012)

Abstract | Full text | PDF | PubMed

25.

749
Accesses

Research article   Open Access

Stable high volumetric production of glycosylated human recombinant IFNalpha2b in HEK293 cells

Martin Loignon, Sylvie Perret, John Kelly, Denise Boulais, Brian Cass, Louis Bisson, Fatemeh Afkhamizarreh, Yves Durocher BMC Biotechnology 2008, 8:65 (27 August 2008)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

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