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	1. 1823 Accesses	Research article Generation and functional characterization of the anti-transferrin receptor single-chain antibody-GAL4 (TfRscFv-GAL4) fusion protein Qing Ye, Heyu Hu, Zhihua Wang, Tong Lu, Zhiquan Hu, Xing Zeng, Shu Zhang, Jing Liu, Ping Lei, Cong-Yi Wang, Zhangqun Ye, Guanxin Shen BMC Biotechnology 2012, 12:91 (28 November 2012) Abstract \| Full text \| PDF \| PubMed
	2. 1614 Accesses	Research article Domain-swapping of mesophilic xylanase with hyper-thermophilic glucanase Liangwei Liu, Linmin Wang, Zhang Zhang, Xiaodan Guo, Xiangqian Li, Hongge Chen BMC Biotechnology 2012, 12:28 (7 June 2012) Abstract \| Full text \| PDF \| PubMed
	3. 1421 Accesses	Research article Isolation and characterization of Wharton’s jelly-derived multipotent mesenchymal stromal cells obtained from bovine umbilical cord and maintained in a defined serum-free three-dimensional system Tereza C Cardoso, Heitor F Ferrari, Andrea F Garcia, Juliana B Novais, Camila Silva-Frade, Marina C Ferrarezi, Alexandre L Andrade, Roberto Gameiro BMC Biotechnology 2012, 12:18 (4 May 2012) Abstract \| Full text \| PDF \| PubMed
	4. 1422 Accesses	Research article Glycoside Hydrolases from a targeted Compost Metagenome, activity-screening and functional characterization Michael J Dougherty, Patrik D’haeseleer, Terry C Hazen, Blake A Simmons, Paul D Adams, Masood Z Hadi BMC Biotechnology 2012, 12:38 (3 July 2012) Abstract \| Full text \| PDF \| PubMed
	5. 1405 Accesses	Research article Internalization of novel non-viral vector TAT-streptavidin into human cells Johanna Rinne, Brian Albarran, Juulia Jylhävä, Teemu O Ihalainen, Pasi Kankaanpää, Vesa P Hytönen, Patrick S Stayton, Markku S Kulomaa, Maija Vihinen-Ranta BMC Biotechnology 2007, 7:1 (2 January 2007) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	6. 1297 Accesses	Research article Self-assembling Fmoc dipeptide hydrogel for in situ 3D cell culturing Thomas Liebmann, Susanna Rydholm, Victor Akpe, Hjalmar Brismar BMC Biotechnology 2007, 7:88 (10 December 2007) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	7. 987 Accesses	Research article Cloning, overexpression, purification, and characterization of a polyextremophilic β-galactosidase from the Antarctic haloarchaeon Halorubrum lacusprofundi Ram Karan, Melinda D Capes, Priya DasSarma, Shiladitya DasSarma BMC Biotechnology 2013, 13:3 (16 January 2013) Abstract \| Full text \| PDF \| PubMed
	8. 860 Accesses	Research article Genetic engineering of Pyrococcus furiosus to use chitin as a carbon source Martina Kreuzer, Karolin Schmutzler, Ingrid Waege, Michael Thomm, Winfried Hausner BMC Biotechnology 2013, 13:9 (7 February 2013) Abstract \| Full text \| PDF \| PubMed
	9. 833 Accesses	Methodology article An efficient one-step site-directed deletion, insertion, single and multiple-site plasmid mutagenesis protocol Huanting Liu, James H Naismith BMC Biotechnology 2008, 8:91 (4 December 2008) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	10. 767 Accesses	Research article Facile, high efficiency immobilization of lipase enzyme on magnetic iron oxide nanoparticles via a biomimetic coating Yuhong Ren, Jose G Rivera, Lihong He, Harsha Kulkarni, Dong-Keun Lee, Phillip B Messersmith BMC Biotechnology 2011, 11:63 (8 June 2011) Abstract \| Full text \| PDF \| PubMed \| Editor’s summary Lipase can be immobilized efficiently under aqueous conditions by coating magnetic nanoparticles with a polydopamine biomimetic inspired by mussel adhesive proteins, with the enzyme still retaining high activity even after many cycles of magnetic separation and reuse.
	11. 760 Accesses	Research article A 28 nt long synthetic 5^′UTR (synJ) as an enhancer of transgene expression in dicotyledonous plants Shaveta Kanoria, Pradeep Kumar Burma BMC Biotechnology 2012, 12:85 (10 November 2012) Abstract \| Full text \| PDF \| PubMed
	12. 733 Accesses	Methodology article A high-throughput cell migration assay using scratch wound healing, a comparison of image-based readout methods Justin C Yarrow, Zachary E Perlman, Nicholas J Westwood, Timothy J Mitchison BMC Biotechnology 2004, 4:21 (9 September 2004) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central \| F1000 Biology \| Editor’s summary Adaptation of the scratch wound-healing assay to a 384 well-plate format enables a high throughput analysis of cell migration and other cell processes involved in wound healing.
	13. 708 Accesses	Research article Characterization of a newly identified rice chitinase-like protein (OsCLP) homologous to xylanase inhibitor Jingni Wu, Yiming Wang, Sun Tae Kim, Sang Gon Kim, Kyu Young Kang BMC Biotechnology 2013, 13:4 (18 January 2013) Abstract \| Full text \| PDF \| PubMed
	14. 705 Accesses	Methodology article A new method to customize protein expression vectors for fast, efficient and background free parallel cloning Judith Scholz, Hüseyin Besir, Claudia Strasser, Sabine Suppmann BMC Biotechnology 2013, 13:12 (14 February 2013) Abstract \| Provisional PDF \| PubMed
	15. 699 Accesses	Methodology article An asymmetric PCR-based, reliable and rapid single-tube native DNA engineering strategy Yanzhen Bi, Xianfeng Qiao, Zaidong Hua, Liping Zhang, Ximei Liu, Li Li, Wenjun Hua, Hongwei Xiao, Jingrong Zhou, Qingxin Wei, Xinmin Zheng BMC Biotechnology 2012, 12:39 (6 July 2012) Abstract \| Full text \| PDF \| PubMed
	16. 686 Accesses	Methodology article Graded or threshold response of the tet-controlled gene expression: all depends on the concentration of the transactivator Niels Heinz, Katharina Hennig, Rainer Loew BMC Biotechnology 2013, 13:5 (22 January 2013) Abstract \| Full text \| PDF \| PubMed
	17. 684 Accesses	Methodology article Real-time PCR based on SYBR-Green I fluorescence: An alternative to the TaqMan assay for a relative quantification of gene rearrangements, gene amplifications and micro gene deletions Frederique Ponchel, Carmel Toomes, Kieran Bransfield, Fong T Leong, Susan H Douglas, Sarah L Field, Sandra M Bell, Valerie Combaret, Alain Puisieux, Alan J Mighell, Philip A Robinson, Chris F Inglehearn, John D Isaacs, Alex F Markham BMC Biotechnology 2003, 3:18 (13 October 2003) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	18. 666 Accesses	Research article Dynamic strain scanning optimization: an efficient strain design strategy for balanced yield, titer, and productivity. DySScO strategy for strain design Kai Zhuang, Laurence Yang, William R Cluett, Radhakrishnan Mahadevan BMC Biotechnology 2013, 13:8 (6 February 2013) Abstract \| Full text \| PDF \| PubMed
	19. 640 Accesses	Research article Layer-shaped alginate hydrogels enhance the biological performance of human adipose-derived stem cells Bianca Galateanu, Doina Dimonie, Eugeniu Vasile, Sorin Nae, Anisoara Cimpean, Marieta Costache BMC Biotechnology 2012, 12:35 (29 June 2012) Abstract \| Full text \| PDF \| PubMed
	20. 639 Accesses	Methodology article Stable nuclear transformation of Eudorina elegans Kai Lerche, Armin Hallmann BMC Biotechnology 2013, 13:11 (12 February 2013) Abstract \| Full text \| PDF \| PubMed
	21. 572 Accesses	Research article Fibres from flax overproducing beta-1,3-glucanase show increased accumulation of pectin and phenolics and thus higher antioxidant capacity Wioleta Wojtasik, Anna Kulma, Lucyna Dymi¿ska, Jerzy Hanuza, Jacek ¿ebrowski, Jan Szopa BMC Biotechnology 2013, 13:10 (9 February 2013) Abstract \| Provisional PDF \| PubMed
	22. 559 Accesses	Methodology article Simple and efficient site-directed mutagenesis using two single-primer reactions in parallel to generate mutants for protein structure-function studies Oded Edelheit, Aaron Hanukoglu, Israel Hanukoglu BMC Biotechnology 2009, 9:61 (30 June 2009) Abstract \| Full text \| PDF \| PubMed
	23. 560 Accesses	Research article Oil accumulation in the model green alga Chlamydomonas reinhardtii: characterization, variability between common laboratory strains and relationship with starch reserves Magali Siaut, Stéphan Cuiné, Caroline Cagnon, Boris Fessler, Mai Nguyen, Patrick Carrier, Audrey Beyly, Fred Beisson, Christian Triantaphylidès, Yonghua Li-Beisson, Gilles Peltier BMC Biotechnology 2011, 11:7 (21 January 2011) Abstract \| Full text \| PDF \| PubMed
	24. 550 Accesses	Methodology article A comparison between two brine shrimp assays to detect in vitro cytotoxicity in marine natural products José Carballo, Zaira L Hernández-Inda, Pilar Pérez, María D García-Grávalos BMC Biotechnology 2002, 2:17 (23 September 2002) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	25. 543 Accesses	Research article Development of a versatile TaqManTM real-time quantitative PCR (RT-qPCR) compliant anchor sequence to quantify bacterial gene transcripts from RNA samples containing carryover genomic DNA Vijay J Gadkar, Martin Filion BMC Biotechnology 2013, 13:7 (31 January 2013) Abstract \| Provisional PDF \| PubMed