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	1.	Research article Killing cancer cells by targeted drug-carrying phage nanomedicines Hagit Bar, Iftach Yacoby, Itai Benhar BMC Biotechnology 2008, 8:37 (3 April 2008) Abstract \| Full text \| PDF \| PubMed \| Editor’s summary Genetically modified, filamentous bacteriophage act as novel chemotherapy-delivery platforms, with nanoparticles using anti-ErbB2 antibodies to target mammary cancer cells in vitro, offering the promise of clinical cancer therapy with fewer systemic side-effects.
	2.	Methodology article An efficient one-step site-directed deletion, insertion, single and multiple-site plasmid mutagenesis protocol Huanting Liu, James H Naismith BMC Biotechnology 2008, 8:91 (4 December 2008) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	3.	Research article Viral and murine interleukin-10 are correctly processed and retain their biological activity when produced in tobacco Luisa Bortesi, Marzia Rossato, Flora Schuster, Nicole Raven, Johannes Stadlmann, Linda Avesani, Alberto Falorni, Flavia Bazzoni, Ralph Bock, Stefan Schillberg, Mario Pezzotti BMC Biotechnology 2009, 9:22 (19 March 2009) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central \| Editor’s summary The efficient production of biologically-active murine and viral Interleukin-10 (IL-10) in transgenic tobacco paves the way for oral administration of this anti-inflammatory cytokine in animal studies of autoimmune disease.
	4.	Research article Pseudomonas fluorescens biofilms subjected to phage phiIBB-PF7A Sanna Sillankorva, Peter Neubauer, Joana Azeredo BMC Biotechnology 2008, 8:79 (27 October 2008) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	5.	Methodology article Real-time PCR based on SYBR-Green I fluorescence: An alternative to the TaqMan assay for a relative quantification of gene rearrangements, gene amplifications and micro gene deletions Frederique Ponchel, Carmel Toomes, Kieran Bransfield, Fong T Leong, Susan H Douglas, Sarah L Field, Sandra M Bell, Valerie Combaret, Alain Puisieux, Alan J Mighell, Philip A Robinson, Chris F Inglehearn, John D Isaacs, Alex F Markham BMC Biotechnology 2003, 3:18 (13 October 2003) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	6.	Research article Measuring microRNAs: Comparisons of microarray and quantitative PCR measurements, and of different total RNA prep methods Robert A Ach, Hui Wang, Bo Curry BMC Biotechnology 2008, 8:69 (11 September 2008) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	7.	Research article Antibody expressing pea seeds as fodder for prevention of gastrointestinal parasitic infections in chickens Jana Zimmermann, Isolde Saalbach, Doreen Jahn, Martin Giersberg, Sigrun Haehnel, Julia Wedel, Jeanette Macek, Karen Zoufal, Gerhard Glünder, Dieter Falkenburg, Sergey M Kipriyanov BMC Biotechnology 2009, 9:79 (11 September 2009) Abstract \| Full text \| PDF \| PubMed \| 1 comment \| Editor’s summary Chickens are protected from developing the economically-important illness coccidiosis by eating pea seeds expressing antibodies against the parasite Eimeria, showing passive immunization to be a cost-effective method of controlling this infectious disease.
	8.	Methodology article A kinetic-based sigmoidal model for the polymerase chain reaction and its application to high-capacity absolute quantitative real-time PCR Robert G Rutledge, Don Stewart BMC Biotechnology 2008, 8:47 (8 May 2008) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central \| 1 comment \| Editor’s summary The LRE (linear regression of efficiency) algorithm for estimating PCR efficiency is a kinetic-based sigmoidal approach that, when coupled with optical calibration, provides data comparison without using standard curves, allowing high-capacity absolute PCR quantification.
	9.	Methodology article PCR-based gene synthesis to produce recombinant proteins for crystallization Damien Marsic, Ronny C Hughes, Miranda L Byrne-Steele, Joseph D Ng BMC Biotechnology 2008, 8:44 (29 April 2008) Abstract \| Full text \| PDF \| PubMed
	10.	Methodology article FastCloning: a highly simplified, purification-free, sequence- and ligation-independent PCR cloning method Chaokun Li, Aiyun Wen, Benchang Shen, Jia Lu, Yao Huang, Yongchang Chang BMC Biotechnology 2011, 11:92 (12 October 2011) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	11.	Research article Preparation of alginate coated chitosan microparticles for vaccine delivery XingYi Li, XiangYe Kong, Shuai Shi, XiuLing Zheng, Gang Guo, YuQuan Wei, ZhiYong Qian BMC Biotechnology 2008, 8:89 (19 November 2008) Abstract \| Full text \| PDF \| PubMed \| Editor’s summary Providing chitosan microparticles with a coating of alginate improves their potential ability to deliver mucosal vaccines orally by both reducing the degradation of attached antigens by gastric juices and by delaying antigen release.
	12.	Research article Electrospun micro- and nanofiber tubes for functional nervous regeneration in sciatic nerve transections Silvia Panseri, Carla Cunha, Joseph Lowery, Ubaldo Del Carro, Francesca Taraballi, Stefano Amadio, Angelo Vescovi, Fabrizio Gelain BMC Biotechnology 2008, 8:39 (11 April 2008) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	13.	Methodology article Simultaneous detection of mRNA and protein stem cell markers in live cells Won Jong Rhee, Gang Bao BMC Biotechnology 2009, 9:30 (2 April 2009) Abstract \| Full text \| PDF \| PubMed
	14.	Methodology article Improved Tet-responsive promoters with minimized background expression Rainer Loew, Niels Heinz, Mathias Hampf, Hermann Bujard, Manfred Gossen BMC Biotechnology 2010, 10:81 (24 November 2010) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	15.	Methodology article A comparison between two brine shrimp assays to detect in vitro cytotoxicity in marine natural products José Carballo, Zaira L Hernández-Inda, Pilar Pérez, María D García-Grávalos BMC Biotechnology 2002, 2:17 (23 September 2002) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	16.	Research article Critical evaluation of HPV16 gene copy number quantification by SYBR green PCR Ian Roberts, Grace Ng, Nicola Foster, Margaret Stanley, Michael T Herdman, Mark R Pett, Andrew Teschendorff, Nicholas Coleman BMC Biotechnology 2008, 8:57 (24 July 2008) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central \| 1 comment \| Editor’s summary Assessment of HPV16 load in cervical cancer using SYBR green PCR produces unavoidable inaccuracies, but calibration using a HPV16 E6 gene construct gives a useful indication of viral load.
	17.	Research article Microporation is a valuable transfection method for efficient gene delivery into human umbilical cord blood-derived mesenchymal stem cells Jung Lim, Sun Park, Chang Jeong, Ji Oh, Seong Kim, Chung Ryu, Soon A Park, Jae Ahn, Wonil Oh, Sin-Soo Jeun, Jong Chang BMC Biotechnology 2010, 10:38 (13 May 2010) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	18.	Research article Cloning, overexpression, purification, and characterization of a polyextremophilic β-galactosidase from the Antarctic haloarchaeon Halorubrum lacusprofundi Ram Karan, Melinda D Capes, Priya DasSarma, Shiladitya DasSarma BMC Biotechnology 2013, 13:3 (16 January 2013) Abstract \| Full text \| PDF \| PubMed
	19.	Research article Collagen matrices from sponge to nano: new perspectives for tissue engineering of skeletal muscle Justus P Beier, Dorothee Klumpp, Markus Rudisile, Roland Dersch, Joachim H Wendorff, Oliver Bleiziffer, Andreas Arkudas, Elias Polykandriotis, Raymund E Horch, Ulrich Kneser BMC Biotechnology 2009, 9:34 (15 April 2009) Abstract \| Full text \| PDF \| PubMed
	20.	Research article Generation of Cell Lines to Complement Adenovirus Vectors using Recombination-Mediated Cassette Exchange Susan J Morris, Daniel C Farley, Keith N Leppard BMC Biotechnology 2010, 10:92 (23 December 2010) Abstract \| Full text \| PDF \| PubMed
	21.	Methodology article Construction of a series of vectors for high throughput cloning and expression screening of membrane proteins from Mycobacterium tuberculosis Huajun Qin, Jian Hu, Yuanzhi Hua, Shridhar V Challa, Timothy A Cross, Fei P Gao BMC Biotechnology 2008, 8:51 (16 May 2008) Abstract \| Full text \| PDF \| PubMed
	22.	Methodology article Immunohistochemical detection of transgene expression in the brain using small epitope tags Evy Lobbestael, Veerle Reumers, Abdelilah Ibrahimi, Kirsten Paesen, Irina Thiry, Rik Gijsbers, Chris Van den Haute, Zeger Debyser, Veerle Baekelandt, Jean-Marc Taymans BMC Biotechnology 2010, 10:16 (18 February 2010) Abstract \| Full text \| PDF \| PubMed
	23.	Research article Oil accumulation in the model green alga Chlamydomonas reinhardtii: characterization, variability between common laboratory strains and relationship with starch reserves Magali Siaut, Stéphan Cuiné, Caroline Cagnon, Boris Fessler, Mai Nguyen, Patrick Carrier, Audrey Beyly, Fred Beisson, Christian Triantaphylidès, Yonghua Li-Beisson, Gilles Peltier BMC Biotechnology 2011, 11:7 (21 January 2011) Abstract \| Full text \| PDF \| PubMed
	24.	Research article An intein with genetically selectable markers provides a new approach to internally label proteins with GFP Richard Ramsden, Luther Arms, Trisha N Davis, Eric GD Muller BMC Biotechnology 2011, 11:71 (27 June 2011) Abstract \| Full text \| PDF \| PubMed
	25.	Research article Jellyfish mucin may have potential disease-modifying effects on osteoarthritis Naoshi Ohta, Masato Sato, Kiminori Ushida, Mami Kokubo, Takayuki Baba, Kayoko Taniguchi, Makoto Urai, Koji Kihira, Joji Mochida BMC Biotechnology 2009, 9:98 (8 December 2009) Abstract \| Full text \| PDF \| PubMed