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1.

Research article   Open Access

Generation of Cell Lines to Complement Adenovirus Vectors using Recombination-Mediated Cassette Exchange

Susan J Morris, Daniel C Farley, Keith N Leppard BMC Biotechnology 2010, 10:92 (23 December 2010)

Abstract | Full text | PDF | PubMed

2.

Research article   Open Access

Vitrification in Open and Closed Carriers at Different Cell Stages: Assessment of Embryo Survival, Development, DNA Integrity and Stability during Vapor Phase Storage for Transport

Faten AbdelHafez, Jing Xu, Jeffrey Goldberg, Nina Desai BMC Biotechnology 2011, 11:29 (30 March 2011)

Abstract | Full text | PDF | PubMed

3.

Methodology article   Open Access Highly Accessed

Specific and sensitive quantitative RT-PCR of miRNAs with DNA primers

Ingrid Balcells, Susanna Cirera, Peter K Busk BMC Biotechnology 2011, 11:70 (25 June 2011)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central |  Editor’s summary

The use of DNA primers, rather than locked nucleic acids (LNA)-spiked primers, in microRNA-specific quantitative RT-PCR (qPCR) leads to higher amplification efficiency and translates into higher sensitivity and precision in microRNA quantification.

4.

Research article   Open Access

Serum-free microcarrier based production of replication deficient Influenza vaccine candidate virus lacking NS1 using Vero cells

Allen Chen, Swan Li Poh, Christian Dietzsch, Elisabeth Roethl, Mylene L Yan, Say Kong Ng BMC Biotechnology 2011, 11:81 (11 August 2011)

Abstract | Full text | PDF | PubMed

5.

Methodology article   Open Access Highly Accessed

A high-throughput cell migration assay using scratch wound healing, a comparison of image-based readout methods

Justin C Yarrow, Zachary E Perlman, Nicholas J Westwood, Timothy J Mitchison BMC Biotechnology 2004, 4:21 (9 September 2004)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central | F1000 Biology |  Editor’s summary

Adaptation of the scratch wound-healing assay to a 384 well-plate format enables a high throughput analysis of cell migration and other cell processes involved in wound healing.

6.

Methodology article   Open Access Highly Accessed

Simple and efficient site-directed mutagenesis using two single-primer reactions in parallel to generate mutants for protein structure-function studies

Oded Edelheit, Aaron Hanukoglu, Israel Hanukoglu BMC Biotechnology 2009, 9:61 (30 June 2009)

Abstract | Full text | PDF | PubMed

7.

Methodology article   Open Access

An improved phage-display panning method to produce an HM-1 killer toxin anti-idiotypic antibody

M Enamul Kabir, Senthilkumar Krishnaswamy, Masahiko Miyamoto, Yasuhiro Furuichi, Tadazumi Komiyama BMC Biotechnology 2009, 9:99 (14 December 2009)

Abstract | Full text | PDF | PubMed

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