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Generation of Cell Lines to Complement Adenovirus Vectors using Recombination-Mediated Cassette Exchange
Susan J Morris, Daniel C Farley, Keith N Leppard BMC Biotechnology 2010, 10:92 (23 December 2010)
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| PubMed
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Vitrification in Open and Closed Carriers at Different Cell Stages: Assessment of Embryo Survival, Development, DNA Integrity and Stability during Vapor Phase Storage for Transport
Faten AbdelHafez, Jing Xu, Jeffrey Goldberg, Nina Desai BMC Biotechnology 2011, 11:29 (30 March 2011)
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| PubMed
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Specific and sensitive quantitative RT-PCR of miRNAs with DNA primers
Ingrid Balcells, Susanna Cirera, Peter K Busk BMC Biotechnology 2011, 11:70 (25 June 2011)
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| PubMed | Cited on BioMed Central
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Editor’s summary
The use of DNA primers, rather than locked nucleic acids (LNA)-spiked primers, in microRNA-specific quantitative RT-PCR (qPCR) leads to higher amplification efficiency and translates into higher sensitivity and precision in microRNA quantification.
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Serum-free microcarrier based production of replication deficient Influenza vaccine candidate virus lacking NS1 using Vero cells
Allen Chen, Swan Li Poh, Christian Dietzsch, Elisabeth Roethl, Mylene L Yan, Say Kong Ng BMC Biotechnology 2011, 11:81 (11 August 2011)
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| PubMed
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A high-throughput cell migration assay using scratch wound healing, a comparison of image-based readout methods
Justin C Yarrow, Zachary E Perlman, Nicholas J Westwood, Timothy J Mitchison BMC Biotechnology 2004, 4:21 (9 September 2004)
Abstract | Full text | PDF
| PubMed | Cited on BioMed Central
| F1000 Biology
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Editor’s summary
Adaptation of the scratch wound-healing assay to a 384 well-plate format enables a high throughput analysis of cell migration and other cell processes involved in wound healing.
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Simple and efficient site-directed mutagenesis using two single-primer reactions in parallel to generate mutants for protein structure-function studies
Oded Edelheit, Aaron Hanukoglu, Israel Hanukoglu BMC Biotechnology 2009, 9:61 (30 June 2009)
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An improved phage-display panning method to produce an HM-1 killer toxin anti-idiotypic antibody
M Enamul Kabir, Senthilkumar Krishnaswamy, Masahiko Miyamoto, Yasuhiro Furuichi, Tadazumi Komiyama BMC Biotechnology 2009, 9:99 (14 December 2009)
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| PubMed
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