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1.

Review   Open Access Highly Accessed

Less is more: strategies to remove marker genes from transgenic plants

Yuan-Yeu Yau, C Neal Stewart BMC Biotechnology 2013, 13:36 (23 April 2013)

Abstract | Provisional PDF | PubMed |  Editor’s summary

Yuan-Yeu Yau and C Neal Stewart review existing strategies to remove selectable marker genes from transgenic plants, including site specific recombination systems, and explore emerging technologies for increased precision genome modification.

2.

Research article   Open Access

Immobilized Rhizopus oryzae lipase catalyzed synthesis of palm stearin and cetyl alcohol wax esters: Optimization by Response Surface Methodology

Mohamed Sellami, Imen Aissa, Fakher Frikha, Youssef Gargouri, Nabil Miled BMC Biotechnology 2011, 11:68 (17 June 2011)

Abstract | Full text | PDF | PubMed

3.

Research article   Open Access

Protein body formation in stable transgenic tobacco expressing elastin-like polypeptide and hydrophobin fusion proteins

Sonia P GutiƩrrez, Reza Saberianfar, Susanne E Kohalmi, Rima Menassa BMC Biotechnology 2013, 13:40 (10 May 2013)

Abstract | Full text | PDF | PubMed

4.

Methodology article   Open Access

Quantification of PRL/Stat5 signaling with a novel pGL4-CISH reporter

Feng Fang, Giovanni Antico, Jiamao Zheng, Charles V Clevenger BMC Biotechnology 2008, 8:11 (6 February 2008)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

5.

Research article   Open Access Highly Accessed

Novel sequential ChIP and simplified basic ChIP protocols for promoter co-occupancy and target gene identification in human embryonic stem cells

Ricardo B Medeiros, Kate J Papenfuss, Brian Hoium, Kristen Coley, Joy Jadrich, Saik-Kia Goh, Anuratha Elayaperumal, Julio E Herrera, Ernesto Resnik, Hsiao-Tzu Ni BMC Biotechnology 2009, 9:59 (29 June 2009)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central |  Editor’s summary

A novel, fast, sequential chromatin immunoprecipitation (ChIP) protocol with high sensitivity identified promoter co-occupancy for transcription factors in human embryonic stem cells, providing a useful high-throughput tool to investigate protein–DNA interactions.

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