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Methodology article   Open Access

Oligonucleotide indexing of DNA barcodes: identification of tuna and other scombrid species in food products

Sara Botti, Elisabetta Giuffra BMC Biotechnology 2010, 10:60 (23 August 2010)

Abstract | Full text | PDF | PubMed

Research article   Open Access Highly Accessed

Characterization of single chain antibody targets through yeast two hybrid

Ole Vielemeyer, Clément Nizak, Ana Jimenez, Arnaud Echard, Bruno Goud, Jacques Camonis, Jean-Christophe Rain, Franck Perez BMC Biotechnology 2010, 10:59 (22 August 2010)

Abstract | Full text | PDF | PubMed

Research article   Open Access Highly Accessed

Novel strong tissue specific promoter for gene expression in human germ cells

Denis Kuzmin, Elena Gogvadze, Roman Kholodenko, Dawid P Grzela, Maxim Mityaev, Tatyana Vinogradova, Eugene Kopantzev, Galina Malakhova, Maria Suntsova, Dmitry Sokov, Zoltán Ivics, Anton Buzdin BMC Biotechnology 2010, 10:58 (17 August 2010)

Abstract | Full text | PDF | PubMed

Research article   Open Access

Moisture-induced solid state instabilities in α-chymotrypsin and their reduction through chemical glycosylation

Giselle M Flores-Fernández, Miraida Pagán, Mariangely Almenas, Ricardo J Solá, Kai Griebenow BMC Biotechnology 2010, 10:57 (9 August 2010)

Abstract | Full text | PDF | PubMed

Methodology article   Open Access Highly Accessed

Automated seamless DNA co-transformation cloning with direct expression vectors applying positive or negative insert selection

Natacha Olieric, Melanie Kuchen, Sandro Wagen, Marion Sauter, Stephanie Crone, Sonia Edmondson, Daniel Frey, Christian Ostermeier, Michel O Steinmetz, Rolf Jaussi BMC Biotechnology 2010, 10:56 (9 August 2010)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

Research article   Open Access

Testing the interaction between analytical modules: an example with Roundup Ready® soybean line GTS 40-3-2

Gianni Bellocchi, Marzia De Giacomo, Nicoletta Foti, Marco Mazzara, Eleonora Palmaccio, Cristian Savini, Chiara Di Domenicantonio, Roberta Onori, Guy Van den Eede BMC Biotechnology 2010, 10:55 (5 August 2010)

Abstract | Full text | PDF | PubMed

Research article   Open Access

SIVsm Tat, Rev, and Nef1: functional characteristics of r-GV internalization on isotypes, cytokines, and intracellular degradation

Marinko Sremac, Elizabeth S Stuart BMC Biotechnology 2010, 10:54 (19 July 2010)

Abstract | Full text | PDF | PubMed

Methodology article   Open Access

Using quantitative real-time PCR to detect chimeras in transgenic tobacco and apricot and to monitor their dissociation

Mohamed Faize, Lydia Faize, Lorenzo Burgos BMC Biotechnology 2010, 10:53 (16 July 2010)

Abstract | Full text | PDF | PubMed

Research article   Open Access

Effect of codon optimization and subcellular targeting on Toxoplasma gondii antigen SAG1 expression in tobacco leaves to use in subcutaneous and oral immunization in mice

Melina Laguía-Becher, Valentina Martín, Mauricio Kraemer, Mariana Corigliano, María L Yacono, Alejandra Goldman, Marina Clemente BMC Biotechnology 2010, 10:52 (15 July 2010)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

Research article   Open Access

Exobiopolymer production of Ophiocordyceps dipterigena BCC 2073: optimization, production in bioreactor and characterization

Kanokarn Kocharin, Pranee Rachathewee, Jean-Jacques Sanglier, Wai Prathumpai BMC Biotechnology 2010, 10:51 (12 July 2010)

Abstract | Full text | PDF | PubMed

Methodology article   Open Access Highly Accessed

Ao38, a new cell line from eggs of the black witch moth, Ascalapha odorata (Lepidoptera: Noctuidae), is permissive for AcMNPV infection and produces high levels of recombinant proteins

Yoshifumi Hashimoto, Sheng Zhang, Gary W Blissard BMC Biotechnology 2010, 10:50 (5 July 2010)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

Research article   Open Access

A process optimization for bio-catalytic production of substituted catechols (3-nitrocatechol and 3-methylcatechol

Dhan Prakash, Janmejay Pandey, Bhupendra N Tiwary, Rakesh K Jain BMC Biotechnology 2010, 10:49 (30 June 2010)

Abstract | Full text | PDF | PubMed

Research article   Open Access

Susceptible cell lines for the production of porcine reproductive and respiratory syndrome virus by stable transfection of sialoadhesin and CD163

Iris Delrue, Hanne Van Gorp, Jan Van Doorsselaere, Peter L Delputte, Hans J Nauwynck BMC Biotechnology 2010, 10:48 (29 June 2010)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

Methodology article   Open Access Highly Accessed

Optimization and analysis of a quantitative real-time PCR-based technique to determine microRNA expression in formalin-fixed paraffin-embedded samples

Rashmi S Goswami, Levi Waldron, Jerry Machado, Nilva K Cervigne, Wei Xu, Patricia P Reis, Denis J Bailey, Igor Jurisica, Michael R Crump, Suzanne Kamel-Reid BMC Biotechnology 2010, 10:47 (23 June 2010)

Abstract | Full text | PDF | PubMed

Methodology article   Open Access

A modified fluorimetric host cell reactivation assay to determine the repair capacity of primary keratinocytes, melanocytes and fibroblasts

Katharina Burger, Katja Matt, Nicole Kieser, Daniel Gebhard, Jörg Bergemann BMC Biotechnology 2010, 10:46 (22 June 2010)

Abstract | Full text | PDF | PubMed

Methodology article   Open Access Highly Accessed

Real-time detection of viable microorganisms by intracellular phototautomerism

Remco Kort, Andreas Nocker, Alie de Kat Angelino-Bart, Sjaak van Veen, Herman Verheij, Frank Schuren, Roy Montijn BMC Biotechnology 2010, 10:45 (18 June 2010)

Abstract | Full text | PDF | PubMed |  Editor’s summary

Real-time viable cell detection allows live microorganisms to be detected without cultivation by utilizing the inability of dead microbes to maintain a neutral cytosolic pH which is necessary for the dissociation and fluorescence of a labeled probe.

Research article   Open Access Highly Accessed

α-Amylase inhibitor-1 gene from Phaseolus vulgaris expressed in Coffea arabica plants inhibits α-amylases from the coffee berry borer pest

Aulus EAD Barbosa, Érika VS Albuquerque, Maria CM Silva, Djair SL Souza, Osmundo B Oliveira-Neto, Arnubio Valencia, Thales L Rocha, Maria F Grossi-de-Sa BMC Biotechnology 2010, 10:44 (17 June 2010)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

Methodology article   Open Access

Accurate non-invasive image-based cytotoxicity assays for cultured cells

Patricia Marqués-Gallego, Hans den Dulk, Claude Backendorf, Jaap Brouwer, Jan Reedijk, Julian F Burke BMC Biotechnology 2010, 10:43 (17 June 2010)

Abstract | Full text | PDF | PubMed

Methodology article   Open Access Highly Accessed

Biotinylated-sortase self-cleavage purification (BISOP) method for cell-free produced proteins

Satoko Matsunaga, Kazuhiro Matsuoka, Kouhei Shimizu, Yaeta Endo, Tatsuya Sawasaki BMC Biotechnology 2010, 10:42 (4 June 2010)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

Research article   Open Access

Stable expression and phenotypic impact of attacin E transgene in orchard grown apple trees over a 12 year period

Ewa Borejsza-Wysocka, John L Norelli, Herb S Aldwinckle, Mickael Malnoy BMC Biotechnology 2010, 10:41 (3 June 2010)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central | 1 comment

Research article   Open Access Highly Accessed

Hydrocarbon phenotyping of algal species using pyrolysis-gas chromatography mass spectrometry

Dinesh K Barupal, Tobias Kind, Shankar L Kothari, Do Lee, Oliver Fiehn BMC Biotechnology 2010, 10:40 (21 May 2010)

Abstract | Full text | PDF | PubMed |  Editor’s summary

Hydrocarbon phenotyping of algae can be achieved cost-effectively using substructure annotation algorithms to assess data from pyrolysis-gas chromatography quadrupole mass spectrometry, enabling rapid discrimination between algal strains for biofuel applications.

Research article   Open Access Highly Accessed

The production of fluorescent transgenic trout to study in vitro myogenic cell differentiation

Jean-Charles Gabillard, Cécile Rallière, Nathalie Sabin, Pierre-Yves Rescan BMC Biotechnology 2010, 10:39 (17 May 2010)

Abstract | Full text | PDF | PubMed

Research article   Open Access Highly Accessed

Microporation is a valuable transfection method for efficient gene delivery into human umbilical cord blood-derived mesenchymal stem cells

Jung Lim, Sun Park, Chang Jeong, Ji Oh, Seong Kim, Chung Ryu, Soon A Park, Jae Ahn, Wonil Oh, Sin-Soo Jeun, Jong Chang BMC Biotechnology 2010, 10:38 (13 May 2010)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

Research article   Open Access

Exploitation of the interaction of measles virus fusogenic envelope proteins with the surface receptor CD46 on human cells for microcell-mediated chromosome transfer

Motonobu Katoh, Yasuhiro Kazuki, Kanako Kazuki, Naoyo Kajitani, Masato Takiguchi, Yuji Nakayama, Takafumi Nakamura, Mitsuo Oshimura BMC Biotechnology 2010, 10:37 (6 May 2010)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

Research article   Open Access Highly Accessed

Degradation or excretion of quantum dots in mouse embryonic stem cells

Qing Pi, Wen Zhang, Guang Zhou, Wei Liu, Yilin Cao BMC Biotechnology 2010, 10:36 (6 May 2010)

Abstract | Full text | PDF | PubMed

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