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		Research article Enzymatic activity of a subtilisin homolog, Tk-SP, from Thermococcus kodakarensis in detergents and its ability to degrade the abnormal prion protein Azumi Hirata, Yuki Hori, Yuichi Koga, Jun Okada, Akikazu Sakudo, Kazuyoshi Ikuta, Shigenori Kanaya, Kazufumi Takano BMC Biotechnology 2013, 13:19 (28 February 2013) Abstract \| Provisional PDF
		Research article High level production of tyrosinase in recombinant Escherichia coli Qun Ren, Bernhard Henes, Michael Fairhead, Linda Thöny-Meyer BMC Biotechnology 2013, 13:18 (27 February 2013) Abstract \| Provisional PDF \| PubMed
		Research article Keratinocyte cytoskeletal roles in cell sheet engineering Qi Wei, Daniel Reidler, Min Ye Shen, Hayden Huang BMC Biotechnology 2013, 13:17 (26 February 2013) Abstract \| Provisional PDF \| PubMed
		Research article Expression of a secretory alpha-glucosidase II from Apis cerana indica in Pichia pastoris and its characterization Jirattikarn Kaewmuangmoon, Manlika Kilaso, Ubolsree Leartsakulpanich, Kiyoshi Kimura, Atsuo Kimura, Chanpen Chanchao BMC Biotechnology 2013, 13:16 (18 February 2013) Abstract \| Provisional PDF \| PubMed
		Research article Feather keratin hydrolysates obtained from microbial keratinases: effect on hair fiber Ana Lúcia Villa, Márcia Regina Aragão, Elisabete Pereira dos Santos, Ana Maria Mazotto, Russolina B Zingali, Edilma Paraguai de Souza, Alane Beatriz Vermelho BMC Biotechnology 2013, 13:15 (18 February 2013) Abstract \| Provisional PDF \| PubMed
		Research article Discovery and characterization of a highly efficient enantioselective mandelonitrile hydrolase from Burkholderia cenocepacia J2315 by phylogeny-based enzymatic substrate specificity prediction Hualei Wang, Huihui Sun, Dongzhi Wei BMC Biotechnology 2013, 13:14 (18 February 2013) Abstract \| Provisional PDF \| PubMed
		Research article Molecular cloning of a novel bioH gene from an environmental metagenome encoding a carboxylesterase with exceptional tolerance to organic solvents Yuping Shi, Yingjie Pan, Bailin Li, Wei He, Qunxin She, Lanming Chen BMC Biotechnology 2013, 13:13 (15 February 2013) Abstract \| Full text \| PDF \| PubMed
		Methodology article A new method to customize protein expression vectors for fast, efficient and background free parallel cloning Judith Scholz, Hüseyin Besir, Claudia Strasser, Sabine Suppmann BMC Biotechnology 2013, 13:12 (14 February 2013) Abstract \| Provisional PDF \| PubMed
		Methodology article Stable nuclear transformation of Eudorina elegans Kai Lerche, Armin Hallmann BMC Biotechnology 2013, 13:11 (12 February 2013) Abstract \| Full text \| PDF \| PubMed
		Research article Fibres from flax overproducing beta-1,3-glucanase show increased accumulation of pectin and phenolics and thus higher antioxidant capacity Wioleta Wojtasik, Anna Kulma, Lucyna Dymi¿ska, Jerzy Hanuza, Jacek ¿ebrowski, Jan Szopa BMC Biotechnology 2013, 13:10 (9 February 2013) Abstract \| Provisional PDF \| PubMed
		Research article Genetic engineering of Pyrococcus furiosus to use chitin as a carbon source Martina Kreuzer, Karolin Schmutzler, Ingrid Waege, Michael Thomm, Winfried Hausner BMC Biotechnology 2013, 13:9 (7 February 2013) Abstract \| Full text \| PDF \| PubMed
		Research article Dynamic strain scanning optimization: an efficient strain design strategy for balanced yield, titer, and productivity. DySScO strategy for strain design Kai Zhuang, Laurence Yang, William R Cluett, Radhakrishnan Mahadevan BMC Biotechnology 2013, 13:8 (6 February 2013) Abstract \| Full text \| PDF \| PubMed
		Research article Development of a versatile TaqManTM real-time quantitative PCR (RT-qPCR) compliant anchor sequence to quantify bacterial gene transcripts from RNA samples containing carryover genomic DNA Vijay J Gadkar, Martin Filion BMC Biotechnology 2013, 13:7 (31 January 2013) Abstract \| Provisional PDF \| PubMed
		Research article Chromosomal evolution of Escherichia coli for the efficient production of lycopene Yun-Yan Chen, Hong-Jie Shen, Yan-Yan Cui, Shang-Guang Chen, Zhi-Ming Weng, Ming Zhao, Jian-Zhong Liu BMC Biotechnology 2013, 13:6 (28 January 2013) Abstract \| Provisional PDF \| PubMed
		Methodology article Graded or threshold response of the tet-controlled gene expression: all depends on the concentration of the transactivator Niels Heinz, Katharina Hennig, Rainer Loew BMC Biotechnology 2013, 13:5 (22 January 2013) Abstract \| Full text \| PDF \| PubMed
		Research article Characterization of a newly identified rice chitinase-like protein (OsCLP) homologous to xylanase inhibitor Jingni Wu, Yiming Wang, Sun Tae Kim, Sang Gon Kim, Kyu Young Kang BMC Biotechnology 2013, 13:4 (18 January 2013) Abstract \| Full text \| PDF \| PubMed
		Research article Cloning, overexpression, purification, and characterization of a polyextremophilic β-galactosidase from the Antarctic haloarchaeon Halorubrum lacusprofundi Ram Karan, Melinda D Capes, Priya DasSarma, Shiladitya DasSarma BMC Biotechnology 2013, 13:3 (16 January 2013) Abstract \| Full text \| PDF \| PubMed
		Correspondence ISA-TAB-Nano: A Specification for Sharing Nanomaterial Research Data in Spreadsheet-based Format Dennis G Thomas, Sharon Gaheen, Stacey L Harper, Martin Fritts, Fred Klaessig, Elizabeth Hahn-Dantona, David Paik, Sue Pan, Grace A Stafford, Elaine T Freund, Juli D Klemm, Nathan A Baker BMC Biotechnology 2013, 13:2 (14 January 2013) Abstract \| Provisional PDF \| PubMed
		Methodology article A simplified counter-selection recombineering protocol for creating fluorescent protein reporter constructs directly from C. elegans fosmid genomic clones Nisha Hirani, Marcel Westenberg, Minaxi S Gami, Paul Davis, Ian A Hope, Colin T Dolphin BMC Biotechnology 2013, 13:1 (3 January 2013) Abstract \| Full text \| PDF \| PubMed
		Research article A vaccine grade of yeast Saccharomyces cerevisiae expressing mammalian myostatin Tingting Zhang, Lin Sun, Ying Xin, Lixia Ma, Youyou Zhang, Xin Wang, Kun Xu, Chonghua Ren, Cunfang Zhang, Zhilong Chen, Hanjiang Yang, Zhiying Zhang BMC Biotechnology 2012, 12:97 (19 December 2012) Abstract \| Full text \| PDF \| PubMed
		Research article Expression of recombinant staphylokinase in the methylotrophic yeast Hansenula polymorpha Manal Moussa, Mahmoud Ibrahim, Maria El Ghazaly, Jan Rohde, Stefan Gnoth, Andreas Anton, Frank Kensy, Frank Mueller BMC Biotechnology 2012, 12:96 (19 December 2012) Abstract \| Full text \| PDF \| PubMed
		Methodology article Use of anionic denaturing detergents to purify insoluble proteins after overexpression Benjamin Schlager, Anna Straessle, Ernst Hafen BMC Biotechnology 2012, 12:95 (11 December 2012) Abstract \| Full text \| PDF \| PubMed
		Research article Guanylate-binding protein 1 expression from embryonal endothelial progenitor cells reduces blood vessel density and cellular apoptosis in an axially vascularised tissue-engineered construct Oliver Bleiziffer, Matthias Hammon, Andreas Arkudas, Christian D Taeger, Justus P Beier, Kerstin Amann, Elisabeth Naschberger, Michael Stürzl, Raymund E Horch, Ulrich Kneser BMC Biotechnology 2012, 12:94 (6 December 2012) Abstract \| Provisional PDF \| PubMed
		Research article Multimodal in vivo imaging reveals limited allograft survival, intrapulmonary cell trapping and minimal evidence for ischemia-directed BMSC homing Bert R Everaert, Irene Bergwerf, Nathalie De Vocht, Peter Ponsaerts, Annemie Van Der Linden, Jean-Pierre Timmermans, Christiaan J Vrints BMC Biotechnology 2012, 12:93 (3 December 2012) Abstract \| Full text \| PDF \| PubMed
		Research article Characterization of transferrin receptor-mediated endocytosis and cellular iron delivery of recombinant human serum transferrin from rice (Oryza sativa L.) Deshui Zhang, Hsin-Fang Lee, Steven C Pettit, Jennica L Zaro, Ning Huang, Wei-Chiang Shen BMC Biotechnology 2012, 12:92 (30 November 2012) Abstract \| Full text \| PDF \| PubMed \| Editor’s summary Rice-derived recombinant human transferrin (rhTF) demonstrates similar transferrin receptor (TFR) binding, TFR-mediated endocytosis and cellular iron delivery function to human transferrin (hTF) and may represent a useful, low-cost alternative for biopharmaceutical applications.

	All
		Research article Enzymatic activity of a subtilisin homolog, Tk-SP, from Thermococcus kodakarensis in detergents and its ability to degrade the abnormal prion protein Azumi Hirata, Yuki Hori, Yuichi Koga, Jun Okada, Akikazu Sakudo, Kazuyoshi Ikuta, Shigenori Kanaya, Kazufumi Takano BMC Biotechnology 2013, 13:19 (28 February 2013) Abstract \| Provisional PDF
		Research article High level production of tyrosinase in recombinant Escherichia coli Qun Ren, Bernhard Henes, Michael Fairhead, Linda Thöny-Meyer BMC Biotechnology 2013, 13:18 (27 February 2013) Abstract \| Provisional PDF \| PubMed
		Research article Keratinocyte cytoskeletal roles in cell sheet engineering Qi Wei, Daniel Reidler, Min Ye Shen, Hayden Huang BMC Biotechnology 2013, 13:17 (26 February 2013) Abstract \| Provisional PDF \| PubMed
		Research article Expression of a secretory alpha-glucosidase II from Apis cerana indica in Pichia pastoris and its characterization Jirattikarn Kaewmuangmoon, Manlika Kilaso, Ubolsree Leartsakulpanich, Kiyoshi Kimura, Atsuo Kimura, Chanpen Chanchao BMC Biotechnology 2013, 13:16 (18 February 2013) Abstract \| Provisional PDF \| PubMed
		Research article Feather keratin hydrolysates obtained from microbial keratinases: effect on hair fiber Ana Lúcia Villa, Márcia Regina Aragão, Elisabete Pereira dos Santos, Ana Maria Mazotto, Russolina B Zingali, Edilma Paraguai de Souza, Alane Beatriz Vermelho BMC Biotechnology 2013, 13:15 (18 February 2013) Abstract \| Provisional PDF \| PubMed
		Research article Discovery and characterization of a highly efficient enantioselective mandelonitrile hydrolase from Burkholderia cenocepacia J2315 by phylogeny-based enzymatic substrate specificity prediction Hualei Wang, Huihui Sun, Dongzhi Wei BMC Biotechnology 2013, 13:14 (18 February 2013) Abstract \| Provisional PDF \| PubMed
		Research article Molecular cloning of a novel bioH gene from an environmental metagenome encoding a carboxylesterase with exceptional tolerance to organic solvents Yuping Shi, Yingjie Pan, Bailin Li, Wei He, Qunxin She, Lanming Chen BMC Biotechnology 2013, 13:13 (15 February 2013) Abstract \| Full text \| PDF \| PubMed
		Methodology article A new method to customize protein expression vectors for fast, efficient and background free parallel cloning Judith Scholz, Hüseyin Besir, Claudia Strasser, Sabine Suppmann BMC Biotechnology 2013, 13:12 (14 February 2013) Abstract \| Provisional PDF \| PubMed
		Methodology article Stable nuclear transformation of Eudorina elegans Kai Lerche, Armin Hallmann BMC Biotechnology 2013, 13:11 (12 February 2013) Abstract \| Full text \| PDF \| PubMed
		Research article Fibres from flax overproducing beta-1,3-glucanase show increased accumulation of pectin and phenolics and thus higher antioxidant capacity Wioleta Wojtasik, Anna Kulma, Lucyna Dymi¿ska, Jerzy Hanuza, Jacek ¿ebrowski, Jan Szopa BMC Biotechnology 2013, 13:10 (9 February 2013) Abstract \| Provisional PDF \| PubMed
		Research article Genetic engineering of Pyrococcus furiosus to use chitin as a carbon source Martina Kreuzer, Karolin Schmutzler, Ingrid Waege, Michael Thomm, Winfried Hausner BMC Biotechnology 2013, 13:9 (7 February 2013) Abstract \| Full text \| PDF \| PubMed
		Research article Dynamic strain scanning optimization: an efficient strain design strategy for balanced yield, titer, and productivity. DySScO strategy for strain design Kai Zhuang, Laurence Yang, William R Cluett, Radhakrishnan Mahadevan BMC Biotechnology 2013, 13:8 (6 February 2013) Abstract \| Full text \| PDF \| PubMed
		Research article Development of a versatile TaqManTM real-time quantitative PCR (RT-qPCR) compliant anchor sequence to quantify bacterial gene transcripts from RNA samples containing carryover genomic DNA Vijay J Gadkar, Martin Filion BMC Biotechnology 2013, 13:7 (31 January 2013) Abstract \| Provisional PDF \| PubMed
		Research article Chromosomal evolution of Escherichia coli for the efficient production of lycopene Yun-Yan Chen, Hong-Jie Shen, Yan-Yan Cui, Shang-Guang Chen, Zhi-Ming Weng, Ming Zhao, Jian-Zhong Liu BMC Biotechnology 2013, 13:6 (28 January 2013) Abstract \| Provisional PDF \| PubMed
		Methodology article Graded or threshold response of the tet-controlled gene expression: all depends on the concentration of the transactivator Niels Heinz, Katharina Hennig, Rainer Loew BMC Biotechnology 2013, 13:5 (22 January 2013) Abstract \| Full text \| PDF \| PubMed
		Research article Characterization of a newly identified rice chitinase-like protein (OsCLP) homologous to xylanase inhibitor Jingni Wu, Yiming Wang, Sun Tae Kim, Sang Gon Kim, Kyu Young Kang BMC Biotechnology 2013, 13:4 (18 January 2013) Abstract \| Full text \| PDF \| PubMed
		Research article Cloning, overexpression, purification, and characterization of a polyextremophilic β-galactosidase from the Antarctic haloarchaeon Halorubrum lacusprofundi Ram Karan, Melinda D Capes, Priya DasSarma, Shiladitya DasSarma BMC Biotechnology 2013, 13:3 (16 January 2013) Abstract \| Full text \| PDF \| PubMed
		Correspondence ISA-TAB-Nano: A Specification for Sharing Nanomaterial Research Data in Spreadsheet-based Format Dennis G Thomas, Sharon Gaheen, Stacey L Harper, Martin Fritts, Fred Klaessig, Elizabeth Hahn-Dantona, David Paik, Sue Pan, Grace A Stafford, Elaine T Freund, Juli D Klemm, Nathan A Baker BMC Biotechnology 2013, 13:2 (14 January 2013) Abstract \| Provisional PDF \| PubMed
		Methodology article A simplified counter-selection recombineering protocol for creating fluorescent protein reporter constructs directly from C. elegans fosmid genomic clones Nisha Hirani, Marcel Westenberg, Minaxi S Gami, Paul Davis, Ian A Hope, Colin T Dolphin BMC Biotechnology 2013, 13:1 (3 January 2013) Abstract \| Full text \| PDF \| PubMed
		Research article A vaccine grade of yeast Saccharomyces cerevisiae expressing mammalian myostatin Tingting Zhang, Lin Sun, Ying Xin, Lixia Ma, Youyou Zhang, Xin Wang, Kun Xu, Chonghua Ren, Cunfang Zhang, Zhilong Chen, Hanjiang Yang, Zhiying Zhang BMC Biotechnology 2012, 12:97 (19 December 2012) Abstract \| Full text \| PDF \| PubMed
		Research article Expression of recombinant staphylokinase in the methylotrophic yeast Hansenula polymorpha Manal Moussa, Mahmoud Ibrahim, Maria El Ghazaly, Jan Rohde, Stefan Gnoth, Andreas Anton, Frank Kensy, Frank Mueller BMC Biotechnology 2012, 12:96 (19 December 2012) Abstract \| Full text \| PDF \| PubMed
		Methodology article Use of anionic denaturing detergents to purify insoluble proteins after overexpression Benjamin Schlager, Anna Straessle, Ernst Hafen BMC Biotechnology 2012, 12:95 (11 December 2012) Abstract \| Full text \| PDF \| PubMed
		Research article Guanylate-binding protein 1 expression from embryonal endothelial progenitor cells reduces blood vessel density and cellular apoptosis in an axially vascularised tissue-engineered construct Oliver Bleiziffer, Matthias Hammon, Andreas Arkudas, Christian D Taeger, Justus P Beier, Kerstin Amann, Elisabeth Naschberger, Michael Stürzl, Raymund E Horch, Ulrich Kneser BMC Biotechnology 2012, 12:94 (6 December 2012) Abstract \| Provisional PDF \| PubMed
		Research article Multimodal in vivo imaging reveals limited allograft survival, intrapulmonary cell trapping and minimal evidence for ischemia-directed BMSC homing Bert R Everaert, Irene Bergwerf, Nathalie De Vocht, Peter Ponsaerts, Annemie Van Der Linden, Jean-Pierre Timmermans, Christiaan J Vrints BMC Biotechnology 2012, 12:93 (3 December 2012) Abstract \| Full text \| PDF \| PubMed
		Research article Characterization of transferrin receptor-mediated endocytosis and cellular iron delivery of recombinant human serum transferrin from rice (Oryza sativa L.) Deshui Zhang, Hsin-Fang Lee, Steven C Pettit, Jennica L Zaro, Ning Huang, Wei-Chiang Shen BMC Biotechnology 2012, 12:92 (30 November 2012) Abstract \| Full text \| PDF \| PubMed \| Editor’s summary Rice-derived recombinant human transferrin (rhTF) demonstrates similar transferrin receptor (TFR) binding, TFR-mediated endocytosis and cellular iron delivery function to human transferrin (hTF) and may represent a useful, low-cost alternative for biopharmaceutical applications.