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Enzymatic activity of a subtilisin homolog, Tk-SP, from Thermococcus kodakarensis in detergents and its ability to degrade the abnormal prion protein
Azumi Hirata, Yuki Hori, Yuichi Koga, Jun Okada, Akikazu Sakudo, Kazuyoshi Ikuta, Shigenori Kanaya, Kazufumi Takano BMC Biotechnology 2013, 13:19 (28 February 2013)
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High level production of tyrosinase in recombinant Escherichia coli
Qun Ren, Bernhard Henes, Michael Fairhead, Linda Thöny-Meyer BMC Biotechnology 2013, 13:18 (27 February 2013)
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Keratinocyte cytoskeletal roles in cell sheet engineering
Qi Wei, Daniel Reidler, Min Ye Shen, Hayden Huang BMC Biotechnology 2013, 13:17 (26 February 2013)
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Expression of a secretory alpha-glucosidase II from Apis cerana indica in Pichia pastoris and its characterization
Jirattikarn Kaewmuangmoon, Manlika Kilaso, Ubolsree Leartsakulpanich, Kiyoshi Kimura, Atsuo Kimura, Chanpen Chanchao BMC Biotechnology 2013, 13:16 (18 February 2013)
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Feather keratin hydrolysates obtained from microbial keratinases: effect on hair fiber
Ana Lúcia Villa, Márcia Regina Aragão, Elisabete Pereira dos Santos, Ana Maria Mazotto, Russolina B Zingali, Edilma Paraguai de Souza, Alane Beatriz Vermelho BMC Biotechnology 2013, 13:15 (18 February 2013)
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Discovery and characterization of a highly efficient enantioselective mandelonitrile hydrolase from Burkholderia cenocepacia J2315 by phylogeny-based enzymatic substrate specificity prediction
Hualei Wang, Huihui Sun, Dongzhi Wei BMC Biotechnology 2013, 13:14 (18 February 2013)
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Molecular cloning of a novel bioH gene from an environmental metagenome encoding a carboxylesterase with exceptional tolerance to organic solvents
Yuping Shi, Yingjie Pan, Bailin Li, Wei He, Qunxin She, Lanming Chen BMC Biotechnology 2013, 13:13 (15 February 2013)
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A new method to customize protein expression vectors for fast, efficient and background free parallel cloning
Judith Scholz, Hüseyin Besir, Claudia Strasser, Sabine Suppmann BMC Biotechnology 2013, 13:12 (14 February 2013)
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Stable nuclear transformation of Eudorina elegans
Kai Lerche, Armin Hallmann BMC Biotechnology 2013, 13:11 (12 February 2013)
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Fibres from flax overproducing beta-1,3-glucanase show increased accumulation of pectin and phenolics and thus higher antioxidant capacity
Wioleta Wojtasik, Anna Kulma, Lucyna Dymi¿ska, Jerzy Hanuza, Jacek ¿ebrowski, Jan Szopa BMC Biotechnology 2013, 13:10 (9 February 2013)
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Genetic engineering of Pyrococcus furiosus to use chitin as a carbon source
Martina Kreuzer, Karolin Schmutzler, Ingrid Waege, Michael Thomm, Winfried Hausner BMC Biotechnology 2013, 13:9 (7 February 2013)
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Dynamic strain scanning optimization: an efficient strain design strategy for balanced yield, titer, and productivity. DySScO strategy for strain design
Kai Zhuang, Laurence Yang, William R Cluett, Radhakrishnan Mahadevan BMC Biotechnology 2013, 13:8 (6 February 2013)
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Development of a versatile TaqManTM real-time quantitative PCR (RT-qPCR) compliant anchor sequence to quantify bacterial gene transcripts from RNA samples containing carryover genomic DNA
Vijay J Gadkar, Martin Filion BMC Biotechnology 2013, 13:7 (31 January 2013)
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Chromosomal evolution of Escherichia coli for the efficient production of lycopene
Yun-Yan Chen, Hong-Jie Shen, Yan-Yan Cui, Shang-Guang Chen, Zhi-Ming Weng, Ming Zhao, Jian-Zhong Liu BMC Biotechnology 2013, 13:6 (28 January 2013)
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Graded or threshold response of the tet-controlled gene expression: all depends on the concentration of the transactivator
Niels Heinz, Katharina Hennig, Rainer Loew BMC Biotechnology 2013, 13:5 (22 January 2013)
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Characterization of a newly identified rice chitinase-like protein (OsCLP) homologous to xylanase inhibitor
Jingni Wu, Yiming Wang, Sun Tae Kim, Sang Gon Kim, Kyu Young Kang BMC Biotechnology 2013, 13:4 (18 January 2013)
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Cloning, overexpression, purification, and characterization of a polyextremophilic β-galactosidase from the Antarctic haloarchaeon Halorubrum lacusprofundi
Ram Karan, Melinda D Capes, Priya DasSarma, Shiladitya DasSarma BMC Biotechnology 2013, 13:3 (16 January 2013)
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ISA-TAB-Nano: A Specification for Sharing Nanomaterial Research Data in Spreadsheet-based Format
Dennis G Thomas, Sharon Gaheen, Stacey L Harper, Martin Fritts, Fred Klaessig, Elizabeth Hahn-Dantona, David Paik, Sue Pan, Grace A Stafford, Elaine T Freund, Juli D Klemm, Nathan A Baker BMC Biotechnology 2013, 13:2 (14 January 2013)
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A simplified counter-selection recombineering protocol for creating fluorescent protein reporter constructs directly from C. elegans fosmid genomic clones
Nisha Hirani, Marcel Westenberg, Minaxi S Gami, Paul Davis, Ian A Hope, Colin T Dolphin BMC Biotechnology 2013, 13:1 (3 January 2013)
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A vaccine grade of yeast Saccharomyces cerevisiae expressing mammalian myostatin
Tingting Zhang, Lin Sun, Ying Xin, Lixia Ma, Youyou Zhang, Xin Wang, Kun Xu, Chonghua Ren, Cunfang Zhang, Zhilong Chen, Hanjiang Yang, Zhiying Zhang BMC Biotechnology 2012, 12:97 (19 December 2012)
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Expression of recombinant staphylokinase in the methylotrophic yeast Hansenula polymorpha
Manal Moussa, Mahmoud Ibrahim, Maria El Ghazaly, Jan Rohde, Stefan Gnoth, Andreas Anton, Frank Kensy, Frank Mueller BMC Biotechnology 2012, 12:96 (19 December 2012)
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Use of anionic denaturing detergents to purify insoluble proteins after overexpression
Benjamin Schlager, Anna Straessle, Ernst Hafen BMC Biotechnology 2012, 12:95 (11 December 2012)
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Guanylate-binding protein 1 expression from embryonal endothelial progenitor cells reduces blood vessel density and cellular apoptosis in an axially vascularised tissue-engineered construct
Oliver Bleiziffer, Matthias Hammon, Andreas Arkudas, Christian D Taeger, Justus P Beier, Kerstin Amann, Elisabeth Naschberger, Michael Stürzl, Raymund E Horch, Ulrich Kneser BMC Biotechnology 2012, 12:94 (6 December 2012)
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Multimodal in vivo imaging reveals limited allograft survival, intrapulmonary cell trapping and minimal evidence for ischemia-directed BMSC homing
Bert R Everaert, Irene Bergwerf, Nathalie De Vocht, Peter Ponsaerts, Annemie Van Der Linden, Jean-Pierre Timmermans, Christiaan J Vrints BMC Biotechnology 2012, 12:93 (3 December 2012)
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Characterization of transferrin receptor-mediated endocytosis and cellular iron delivery of recombinant human serum transferrin from rice (Oryza sativa L.)
Deshui Zhang, Hsin-Fang Lee, Steven C Pettit, Jennica L Zaro, Ning Huang, Wei-Chiang Shen BMC Biotechnology 2012, 12:92 (30 November 2012)
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Editor’s summary
Rice-derived recombinant human transferrin (rhTF) demonstrates similar transferrin receptor (TFR) binding, TFR-mediated endocytosis and cellular iron delivery function to human transferrin (hTF) and may represent a useful, low-cost alternative for biopharmaceutical applications.
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