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Editor-in-Chief
Melissa Norton, MD

Biology Editor
Penelope Webb, PhD

In-house Editor
J. Ann Le Good PhD



BMC  BMC Biotechnology, Volume 9


Methodology article
Stable nuclear transformation of Gonium pectorale
Kai Lerche, Armin Hallmann
BMC Biotechnology 2009, 9:64 (10July2009)
[Abstract] [Provisional PDF]

Research article
Production of N-acetyl-D-neuraminic Acid Using Two Sequential Enzymes Overexpressed as Double-Tagged Fusion Proteins
Tzu-Hsien Wang, Ying-Yin Chen, Hsin-Hung Pan, Feng-Pao Wang, Chung-Hsien Cheng, Wen-Chien Lee
BMC Biotechnology 2009, 9:63 (9July2009)
[Abstract] [Provisional PDF] [PubMed] [Related articles]

Research article
A further insight into the biosorption mechanism of Pt() by infrared spectrometry
Zhongyu Lin, Ru Xue, Yiwen Ye, Jianhong Zheng, Zhenling Xu
BMC Biotechnology 2009, 9:62 (6July2009)
[Abstract] [Provisional PDF] [PubMed] [Related articles]

Methodology article
Simple and efficient site-directed mutagenesis using two single-primer reactions in parallel to generate mutants for protein structure-function studies
Oded Edelheit, Aaron Hanukoglu, Israel Hanukoglu
BMC Biotechnology 2009, 9:61 (30June2009)
[Abstract] [Provisional PDF] [PubMed] [Related articles]

Research article
Isolation of a human-like antibody fragment (scFv) that neutralizes ricin biological activity
Thibaut Pelat, Michael Hust, Martha Hale, Marie-Paule Lefranc, Stefan Dubel, Philippe Thullier
BMC Biotechnology 2009, 9:60 (30June2009)
[Abstract] [Provisional PDF] [PubMed] [Related articles]

Research article
Novel sequential ChIP and simplified basic ChIP protocols for promoter co-occupancy and target gene identification in human embryonic stem cells
Ricardo B Medeiros, Kate J Papenfuss, Brian Hoium, Kristen Coley, Joy Jadrich, Saik-Kia Goh, Anuratha Elayaperumal, Julio E Herrera, Ernesto Resnik, Hsiao-Tzu Ni
BMC Biotechnology 2009, 9:59 (29June2009)
[Abstract] [Provisional PDF] [PubMed] [Related articles]

Methodology article
Cell-free protein synthesis energized by slowly-metabolized maltodextrin
Yiran Wang, Y-H. Percival Zhang
BMC Biotechnology 2009, 9:58 (28June2009)
[Abstract] [Provisional PDF] [PubMed] [Related articles]

Methodology article
Interfering ribonucleic acids that suppress expression of multiple unrelated genes
Toby Passioura, Mary M Gozar, Amber Goodchild, Andrew King, Greg M Arndt, Michael Poidinger, Donald J Birkett, Laurent P Rivory
BMC Biotechnology 2009, 9:57 (16June2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Expression of endo-1, 4-beta-xylanase from Trichoderma reesei in Pichia pastoris and functional characterization of the produced enzyme
Jun He, Bing Yu, Keying Zhang, Xuemei Ding, Daiwen Chen
BMC Biotechnology 2009, 9:56 (16June2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
High-titer preparation of Bombyx mori nucleopolyhedrovirus (BmNPV) displaying recombinant protein in silkworm larvae by size exclusion chromatography and its characterization
Tatsuya Kato, Suganthi Lavender Manoha, Shigeyasu Tanaka, Enoch Y Park
BMC Biotechnology 2009, 9:55 (12June2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Synthesis of sialoglycopolypeptide for potentially blocking influenza virus infection using a rat 2,6-sialyltransferase expressed in BmNPV bacmid-injected silkworm larvae
Makoto Ogata, Makoto Nakajima, Tatsuya Kato, Takakiyo Obara, Hirokazu Yagi, Koichi Kato, Taichi Usui, Enoch Y Park
BMC Biotechnology 2009, 9:54 (5June2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Methodology article
Construction of siRNA/miRNA expression vectors based on a one-step PCR process
Jun Xu, Jie Qiong Zeng, Gang Wan, Gui Bin Hu, Hong Yan, Li Xin Ma
BMC Biotechnology 2009, 9:53 (2June2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Methodology article
Integrated application of uniform design and least-squares support vector machines to transfection optimization
Jin-Shui Pan, Mei-Zhu Hong, Qi-Feng Zhou, Jia-Yan Cai, Hua-Zhen Wang, Lin-Kai Luo, De-Qiang Yang, Jing Dong, Hua-Xiu Shi, Jian-Lin Ren
BMC Biotechnology 2009, 9:52 (31May2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
A microarray-based method for the parallel analysis of genotypes and expression profiles of wood-forming tissues in Eucalyptus grandis
Eugenia Barros, Carol-Ann van Staden, Sabine Lezar
BMC Biotechnology 2009, 9:51 (27May2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Methodology article
Cost-effective HRMA pre-sequence typing of clone libraries; application to phage display selection
Barry A Pepers, Menno H Schut, Rolf HAM Vossen, Gert-Jan B van Ommen, Johan T den Dunnen, Willeke MC van Roon-Mom
BMC Biotechnology 2009, 9:50 (22May2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Methodology article
Expanding the multicolor capabilities of basic confocal microscopes by employing red and near-infrared quantum dot conjugates
Lara M Kingeter, Brian C Schaefer
BMC Biotechnology 2009, 9:49 (22May2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Peroxicretion: a novel secretion pathway in the eukaryotic cell
Cees MJ Sagt, Peter J ten Haaft, Ingeborg M Minneboo, Miranda P Hartog, Robbert A Damveld, Jan Metske van der Laan, Michiel Akeroyd, Thibaut J Wenzel, Francisca A Luesken, Marten Veenhuis, Ida van der Klei, Johannes H de Winde
BMC Biotechnology 2009, 9:48 (20May2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Methodology article
Generation of an external guide sequence library for a reverse genetic screen in Caenorhabditis elegans
Qitao Yan, Rui Zhao, Wenlin Zheng, Changxin Yin, Bao Zhang, Wenli Ma
BMC Biotechnology 2009, 9:47 (20May2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Luciferase activity under direct ligand-dependent control of a muscarinic acetylcholine receptor
Doreen Thor, Diana Le Duc, Rainer Strotmann, Torsten Schneberg
BMC Biotechnology 2009, 9:46 (18May2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Methodology article
Fluorescent labeling of NASBA amplified tmRNA molecules for microarray applications
Ott Scheler, Barry Glynn, Sven Parkel, Priit Palta, Kadri Toome, Lauris Kaplinski, Maido Remm, Majella Maher, Ants Kurg
BMC Biotechnology 2009, 9:45 (15May2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

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