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Editor-in-Chief Melissa Norton, MD Biology Editor Penelope Webb, PhD In-house Editor J. Ann Le Good
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| | BMC Biotechnology, Volume 8 | | Research article
    Three-dimensional culture of human meniscal cells: Extracellular matrix and proteoglycan production Helen E Gruber, David Mauerhan, Yin Chow, Jane A Ingram, H James Norton, Edward N Hanley Jr, Yubo Sun BMC Biotechnology 2008, 8:54 (26 June 2008) [Abstract] [Full text] [PDF] [PubMed] [Related articles] Research article
     Expression of HIV-1 antigens in plants as potential subunit vaccines Ann Meyers, Ereck Chakauya, Enid Shephard, Fiona L Tanzer, James Maclean, Alisson Lynch, Anna-Lise Williamson, Edward P Rybicki BMC Biotechnology 2008, 8:53 (23 June 2008) [Abstract] [Full text] [PDF] [PubMed] [Related articles] Methodology article
    Human IL-12 p40 as a reporter gene for high-throughput screening of engineered mouse embryonic stem cells Leonardo D'Aiuto, Clinton S Robison, Margherita Gigante, Edward Nwanegbo, Benjamin Shaffer, Meena Sukhwani, Carlos A Castro, J Richard Chaillet BMC Biotechnology 2008, 8:52 (3 June 2008) [Abstract] [Full text] [PDF] [PubMed] [Related articles] Methodology article
     Construction of a series of vectors for high throughput cloning and expression screening of membrane proteins from Mycobacterium tuberculosis Huajun Qin, Jian Hu, Yuanzhi Hua, Shridhar V Challa, Timothy A Cross, Fei P Gao BMC Biotechnology 2008, 8:51 (16 May 2008) [Abstract] [Full text] [PDF] [PubMed] [Related articles] Research article
    Substantially improved pharmacokinetics of recombinant human butyrylcholinesterase by fusion to human serum albumin Yue-Jin Huang, Paul M Lundy, Anthoula Lazaris, Yue Huang, Hernan Baldassarre, Bin Wang, Carl Turcotte, M lanie C t , Annie Bellemare, Annie S Bilodeau, Sandra Brouillard, Madjid Touati, Peter Herskovits, Isabelle B gin, Nathalie Neveu, Eric Brochu, Janice Pierson, Duncan K Hockley, Douglas M Cerasoli, David E Lenz, Harvey Wilgus, Costas N Karatzas, Solomon Langermann BMC Biotechnology 2008, 8:50 (16 May 2008) [Abstract] [Full text] [PDF] [PubMed] [Related articles] Research article
    Viral vectors based on bidirectional cell-specific mammalian promoters and transcriptional amplification strategy for use in vitro and in vivo Beihui Liu, Julian F Paton, Sergey Kasparov BMC Biotechnology 2008, 8:49 (16 May 2008) [Abstract] [Full text] [PDF] [PubMed] [Related articles] Research article
    Impact of point-mutations on the hybridization affinity of surface-bound DNA/DNA and RNA/DNA oligonucleotide-duplexes: Comparison of single base mismatches and base bulges Thomas Naiser, Oliver Ehler, Jona Kayser, Timo Mai, Wolfgang Michel, Albrecht Ott BMC Biotechnology 2008, 8:48 (13 May 2008) [Abstract] [Full text] [PDF] [PubMed] [Related articles] Methodology article
     A kinetic-based sigmoidal model for the polymerase chain reaction and its application to high-capacity absolute quantitative real-time PCR Robert G Rutledge, Don Stewart BMC Biotechnology 2008, 8:47 (8 May 2008) [Abstract] [Full text] [PDF] [PubMed] [Related articles] Research article
    Hydroxycamptothecin-loaded nanoparticles enhance target drug delivery and anticancer effect Anxun Wang, Su Li BMC Biotechnology 2008, 8:46 (4 May 2008) [Abstract] [Full text] [PDF] [PubMed] [Related articles] Methodology article
    Flow cytometric monitoring of influenza A virus infection in MDCK cells during vaccine production Josef Schulze-Horsel, Yvonne Genzel, Udo Reichl BMC Biotechnology 2008, 8:45 (30 April 2008) [Abstract] [Full text] [PDF] [PubMed] [Related articles] Methodology article
     PCR-based gene synthesis to produce recombinant proteins for crystallization Damien Marsic, Ronny C Hughes, Miranda L Byrne-Steele, Joseph D Ng BMC Biotechnology 2008, 8:44 (29 April 2008) [Abstract] [Full text] [PDF] [PubMed] [Related articles] Research article
    Characterization of a novel non-specific nuclease from thermophilic bacteriophage GBSV1 Qing Song, Xiaobo Zhang BMC Biotechnology 2008, 8:43 (28 April 2008) [Abstract] [Full text] [PDF] [PubMed] [Related articles] Research article
    Differential protein expression following low temperature culture of suspension CHO-K1 cells Niraj Kumar, Patrick Gammell, Paula Meleady, Michael Henry, Martin Clynes BMC Biotechnology 2008, 8:42 (22 April 2008) [Abstract] [Full text] [PDF] [PubMed] [Related articles] Research article
    In vivo site-specific biotinylation of proteins within the secretory pathway using a single vector system Andrea Predonzani, Francesca Arnoldi, Alejandro L pez-Requena, Oscar R Burrone BMC Biotechnology 2008, 8:41 (18 April 2008) [Abstract] [Full text] [PDF] [PubMed] [Related articles] Methodology article
    Simultaneous monitoring of independent gene expression patterns in two types of cocultured fibroblasts with different color-emitting luciferases Takako Noguchi, Masaaki Ikeda, Yoshihiro Ohmiya, Yoshihiro Nakajima BMC Biotechnology 2008, 8:40 (17 April 2008) [Abstract] [Full text] [PDF] [PubMed] [Related articles] Research article
     Electrospun micro- and nanofiber tubes for functional nervous regeneration in sciatic nerve transections Silvia Panseri, Carla Cunha, Joseph Lowery, Ubaldo Del Carro, Francesca Taraballi, Stefano Amadio, Angelo Vescovi, Fabrizio Gelain BMC Biotechnology 2008, 8:39 (11 April 2008) [Abstract] [Full text] [PDF] [PubMed] [Related articles] Methodology article
    Comparison of the developmental potential of 2-week-old preantral follicles derived from vitrified ovarian tissue slices, vitrified whole ovaries and vitrified/transplanted newborn mouse ovaries using the metal surface method Ta-Chin Lin, Jui-Mei Yen, Tsung-Cheng Kuo, Kun-Bing Gong, Kung-Hao Hsu, Teng-Tsao Hsu BMC Biotechnology 2008, 8:38 (4 April 2008) [Abstract] [Full text] [PDF] [PubMed] [Related articles] Research article
     Killing cancer cells by targeted drug-carrying phage nanomedicines Hagit Bar, Iftach Yacoby, Itai Benhar BMC Biotechnology 2008, 8:37 (3 April 2008) [Abstract] [Full text] [PDF] [PubMed] [Related articles] Research article
     Development of patatin knockdown potato tubers using RNA interference (RNAi) technology, for the production of human-therapeutic glycoproteins Yoon-Sik Kim, Yong-Hwa Lee, Hyun-Soon Kim, Mi-Sun Kim, Kyu-Woong Hahn, Jeong-Heon Ko, Hyouk Joung, Jae-Heung Jeon BMC Biotechnology 2008, 8:36 (3 April 2008) [Abstract] [Full text] [PDF] [PubMed] [Related articles] Research article
     Nanopolymers improve delivery of exon skipping oligonucleotides and concomitant dystrophin expression in skeletal muscle of mdx mice Jason H Williams, Rebecca C Schray, Shashank R Sirsi, Gordon J Lutz BMC Biotechnology 2008, 8:35 (2 April 2008) [Abstract] [Full text] [PDF] [PubMed] [Related articles] Research article
    Identification of a GTP-bound Rho specific scFv molecular sensor by phage display selection Marine Goffinet, Patrick Chinestra, Isabelle Lajoie-Mazenc, Claire Medale-Giamarchi, Gilles Favre, Jean-Charles Faye BMC Biotechnology 2008, 8:34 (31 March 2008) [Abstract] [Full text] [PDF] [PubMed] [Related articles] Methodology article
    Improved production of human type II procollagen in the yeast Pichia pastoris in shake flasks by a wireless-controlled fed-batch system Maria Ruottinen, Monika Bollok, Martin K gler, Antje Neubauer, Mirja Krause, Eija-Riitta H m l inen, Johanna Myllyharju, Antti Vasala, Peter Neubauer BMC Biotechnology 2008, 8:33 (27 March 2008) [Abstract] [Full text] [PDF] [PubMed] [Related articles] Methodology article
    A set of ligation-independent in vitro translation vectors for eukaryotic protein production Viola Bard czy, Vikt ria G czi, Tatsuya Sawasaki, Yaeta Endo, Tam s M sz ros BMC Biotechnology 2008, 8:32 (27 March 2008) [Abstract] [Full text] [PDF] [PubMed] [Related articles] Methodology article
    Gene targeting in adult rhesus macaque fibroblasts Daniel T Meehan, Mary Ann Zink, Melissa Mahlen, Marilu Nelson, Warren G Sanger, Shoukhrat M Mitalipov, Don P Wolf, Michel M Ouellette, Robert B Norgren Jr BMC Biotechnology 2008, 8:31 (26 March 2008) [Abstract] [Full text] [PDF] [PubMed] [Related articles] Methodology article
    RNAi-mediated gene silencing in tick synganglia: A proof of concept study Shahid Karim, Bronwyn Kenny, Emily Troiano, Thomas N Mather BMC Biotechnology 2008, 8:30 (26 March 2008) [Abstract] [Full text] [PDF] [PubMed] [Related articles] Research article
    Production of functionally active Penicillium chrysogenum isopenicillin N synthase in the yeast Hansenula polymorpha Loknath Gidijala, Roel AL Bovenberg, Paul Klaassen, Ida J van der Klei, Marten Veenhuis, Jan AKW Kiel BMC Biotechnology 2008, 8:29 (19 March 2008) [Abstract] [Full text] [PDF] [PubMed] [Related articles] Research article
    Design of a zinc finger protein binding a sequence upstream of the A20 gene Yong Wei, Dajun Ying, Chunli Hou, Xiaoping Cui, Chuhong Zhu BMC Biotechnology 2008, 8:28 (19 March 2008) [Abstract] [Full text] [PDF] [PubMed] [Related articles] Methodology article
    Isolation of soybean protein P34 from oil bodies using hydrophobic interaction chromatography Eva Sewekow, Lars Christian Kessler, Andreas Seidel-Morgenstern, Hermann-Josef Rothk tter BMC Biotechnology 2008, 8:27 (11 March 2008) [Abstract] [Full text] [PDF] [PubMed] [Related articles] Methodology article
     Comparison of different real-time PCR chemistries and their suitability for detection and quantification of genetically modified organisms Meti Buh Ga pari , Katarina Cankar, Jana el, Kristina Gruden BMC Biotechnology 2008, 8:26 (6 March 2008) [Abstract] [Full text] [PDF] [PubMed] [Related articles] Research article
    Glycosyltransferase efficiently controls phenylpropanoid pathway Anna Aksamit-Stachurska, Alina Korobczak-Sosna, Anna Kulma, Jan Szopa BMC Biotechnology 2008, 8:25 (5 March 2008) [Abstract] [Full text] [PDF] [PubMed] [Related articles] Research article
    Intracellular delivery of peptides via association with ubiquitin or SUMO-1 coupled to protein transduction domains Anne-Laure Vitte, Pierre Jalinot BMC Biotechnology 2008, 8:24 (29 February 2008) [Abstract] [Full text] [PDF] [PubMed] [Related articles] Research article
    Effects of size and topology of DNA molecules on intracellular delivery with non-viral gene carriers Charlie Yu Ming Hsu, Hasan Uluda BMC Biotechnology 2008, 8:23 (29 February 2008) [Abstract] [Full text] [PDF] [PubMed] [Related articles] Methodology article
    Protein kinase substrate identification on functional protein arrays Lihao Meng, Gregory A Michaud, Janie S Merkel, Fang Zhou, Jing Huang, Dawn R Mattoon, Barry Schweitzer BMC Biotechnology 2008, 8:22 (28 February 2008) [Abstract] [Full text] [PDF] [PubMed] [Related articles] Research article
    Systolic and diastolic ventricular function in zebrafish embryos: Influence of norepenephrine, MS-222 and temperature Martin A Denvir, Carl S Tucker, John J Mullins BMC Biotechnology 2008, 8:21 (27 February 2008) [Abstract] [Full text] [PDF] [PubMed] [Related articles] Research article
    Effect of ionic strength and presence of serum on lipoplexes structure monitorized by FRET Catarina Madeira, Lu s MS Loura, Manuel Prieto, Aleksander Fedorov, M Raquel Aires-Barros BMC Biotechnology 2008, 8:20 (26 February 2008) [Abstract] [Full text] [PDF] [PubMed] [Related articles] Methodology article
    Enhanced gene expression from retroviral vectors Magnus Bl , David R Micklem, James B Lorens BMC Biotechnology 2008, 8:19 (25 February 2008) [Abstract] [Full text] [PDF] [PubMed] [Related articles] Methodology article
    Quantification of Cre-mediated recombination by a novel strategy reveals a stable extra-chromosomal deletion-circle in mice Wouter N Leonhard, Jeroen H Roelfsema, Irma S Lantinga-van Leeuwen, Martijn H Breuning, Dorien JM Peters BMC Biotechnology 2008, 8:18 (25 February 2008) [Abstract] [Full text] [PDF] [PubMed] [Related articles] Methodology article
    Amplification of multiple genomic loci from single cells isolated by laser micro-dissection of tissues Dan Frumkin, Adam Wasserstrom, Shalev Itzkovitz, Alon Harmelin, Gideon Rechavi, Ehud Shapiro BMC Biotechnology 2008, 8:17 (20 February 2008) [Abstract] [Full text] [PDF] [PubMed] [Related articles] Research article
     Simplified approaches for the development of an ELISA to detect circulating autoantibodies to p53 in cancer patients Ratchada Cressey, Saranya Pimpa, Busyamas Chewaskulyong, Nirush Lertprasertsuke, Somchareon Saeteng, Chatchai Tayapiwatana, Watchara Kasinrerk BMC Biotechnology 2008, 8:16 (20 February 2008) [Abstract] [Full text] [PDF] [PubMed] [Related articles] Research article
     Solubility enhancement of aggregation-prone heterologous proteins by fusion expression using stress-responsive Escherichia coli protein, RpoS Jin-Seung Park, Kyung-Yeon Han, Jong-Ho Lee, Jong-Am Song, Keum-Young Ahn, Hyuk-Seong Seo, Sang-Jun Sim, Seung-Wook Kim, Jeewon Lee BMC Biotechnology 2008, 8:15 (19 February 2008) [Abstract] [Full text] [PDF] [PubMed] [Related articles] Research article
    Expression of recombinant Rhipicephalus (Boophilus) microplus, R. annulatus and R. decoloratus Bm86 orthologs as secreted proteins in Pichia pastoris Mario Canales, Jos M P rez de la Lastra, Victoria Naranjo, Ard M Nijhof, Michelle Hope, Frans Jongejan, Jos de la Fuente BMC Biotechnology 2008, 8:14 (14 February 2008) [Abstract] [Full text] [PDF] [PubMed] [Related articles] Research article
    Efficient and reproducible generation of high-expressing, stable human cell lines without need for antibiotic selection Gudrun Schiedner, Sabine Hertel, Corinna Bialek, Helmut Kewes, Gero Wasch tza, Christoph Volpers BMC Biotechnology 2008, 8:13 (12 February 2008) [Abstract] [Full text] [PDF] [PubMed] [Related articles] Methodology article
    High-throughput avian molecular sexing by SYBR green-based real-time PCR combined with melting curve analysis Hsueh-Wei Chang, Chun-An Cheng, De-Leung Gu, Chia-Che Chang, San-Hua Su, Cheng-Hao Wen, Yii-Cheng Chou, Ta-Ching Chou, Cheng-Te Yao, Chi-Li Tsai, Chien-Chung Cheng BMC Biotechnology 2008, 8:12 (12 February 2008) [Abstract] [Full text] [PDF] [PubMed] [Related articles] Methodology article
    Quantification of PRL/Stat5 signaling with a novel pGL4-CISH reporter Feng Fang, Giovanni Antico, Jiamao Zheng, Charles V Clevenger BMC Biotechnology 2008, 8:11 (6 February 2008) [Abstract] [Full text] [PDF] [PubMed] [Related articles] Research article
     Improved RNA quality and TaqMan Pre-amplification method (PreAmp) to enhance expression analysis from formalin fixed paraffin embedded (FFPE) materials Jinghuan Li, Paul Smyth, Susanne Cahill, Karen Denning, Richard Flavin, Sinead Aherne, Marco Pirotta, Simone M Guenther, John J O'Leary, Orla Sheils BMC Biotechnology 2008, 8:10 (6 February 2008) [Abstract] [Full text] [PDF] [PubMed] [Related articles] Research article
    Recombinant gas vesicles from Halobacterium sp. displaying SIV peptides demonstrate biotechnology potential as a pathogen peptide delivery vehicle Marinko Sremac, Elizabeth S Stuart BMC Biotechnology 2008, 8:9 (31 January 2008) [Abstract] [Full text] [PDF] [PubMed] [Related articles] Research article
    Spherezymes: A novel structured self-immobilisation enzyme technology Dean Brady, Justin Jordaan, Clinton Simpson, Avashnee Chetty, Cherise Arumugam, Francis S Moolman BMC Biotechnology 2008, 8:8 (31 January 2008) [Abstract] [Full text] [PDF] [PubMed] [Related articles] Research article
    Laser surgery of zebrafish (Danio rerio) embryos using femtosecond laser pulses: Optimal parameters for exogenous material delivery, and the laser's effect on short- and long-term development Vikram Kohli, Abdulhakem Y Elezzabi BMC Biotechnology 2008, 8:7 (29 January 2008) [Abstract] [Full text] [PDF] [PubMed] [Related articles] Methodology article
    Negative selection of chronic lymphocytic leukaemia cells using a bifunctional rosette-based antibody cocktail Salim Essakali, Dennis Carney, David Westerman, Peter Gambell, John F Seymour, Alexander Dobrovic BMC Biotechnology 2008, 8:6 (29 January 2008) [Abstract] [Full text] [PDF] [PubMed] [Related articles] Research article
    Generation of functional scFv intrabody to abate the expression of CD147 surface molecule of 293A cells Khajornsak Tragoolpua, Nutjeera Intasai, Watchara Kasinrerk, Sabine Mai, Yuan Yuan, Chatchai Tayapiwatana BMC Biotechnology 2008, 8:5 (29 January 2008) [Abstract] [Full text] [PDF] [PubMed] [Related articles] Research article
    Efficient display of active lipase LipB52 with a Pichia pastoris cell surface display system and comparison with the LipB52 displayed on Saccharomyces cerevisiae cell surface Zhengbing Jiang, Bei Gao, Ren Ren, Xingyi Tao, Yushu Ma, Dongzhi Wei BMC Biotechnology 2008, 8:4 (28 January 2008) [Abstract] [Full text] [PDF] [PubMed] [Related articles] Research article
    Enhancement of hybridoma formation, clonability and cell proliferation in a nanoparticle-doped aqueous environment Natalie Gavrilov-Yusim, Ekaterina Hahiashvili, Marina Tashker, Victoria Yavelsky, Ohad Karnieli, Leslie Lobel BMC Biotechnology 2008, 8:3 (14 January 2008) [Abstract] [Full text] [PDF] [PubMed] [Related articles] Research article
    Optimization of an E. coli L-rhamnose-inducible expression vector: test of various genetic module combinations Angelika Wegerer, Tianqi Sun, Josef Altenbuchner BMC Biotechnology 2008, 8:2 (14 January 2008) [Abstract] [Full text] [PDF] [PubMed] [Related articles] Research article
    Quantification of the CBD-FITC conjugates surface coating on cellulose fibres Ricardo Pinto, Ant nio L Amaral, Eug nio C Ferreira, Manuel Mota, Manuel Vilanova, Katia Ruel, Miguel Gama BMC Biotechnology 2008, 8:1 (9 January 2008) [Abstract] [Full text] [PDF] [PubMed] [Related articles]
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