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Editor-in-Chief
Melissa Norton, MD

Biology Editor
Penelope Webb, PhD

In-house Editor
J. Ann Le Good PhD




BMC Biotechnology, Volume 8

 

 

 

Research article
Single-chain Fv phage display propensity exhibits strong positive correlation with overall expression levels
Nathan Scott, Catherine B Reynolds, Michael J Wright, Omar Qazi, Neil Fairweather, Mahendra P Deonarain
BMC Biotechnology 2008, 8:97 (29December2008)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
A modeling study by response surface methodology and artificial neural network on culture parameters optimization for thermostable lipase production from a newly isolated thermophilic Geobacillus sp. strain ARM
Afshin Ebrahimpour, Raja Noor Zaliha Raja Abd Rahman, Diana Hooi Ean Ch'ng, Mahiran Basri, Abu Bakar Salleh
BMC Biotechnology 2008, 8:96 (23December2008)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Hairy-root organ cultures for the production of human acetylcholinesterase
Ryan R Woods, Brian C Geyer, Tsafrir S Mor
BMC Biotechnology 2008, 8:95 (23December2008)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Methodology article
Better estimation of protein-DNA interaction parameters improve prediction of functional sites
Vijayalakshmi H Nagaraj, Ruadhan A O'Flanagan, Anirvan M Sengupta
BMC Biotechnology 2008, 8:94 (23December2008)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Methodology article
Simultaneous tracking of fly movement and gene expression using GFP
Dhruv Grover, Junsheng Yang, Simon Tavar, John Tower
BMC Biotechnology 2008, 8:93 (16December2008)
[Abstract] [Full text] [PDF] [PubMed] [Related articles] [Cited on BioMed Central]

Research article
Large-scale preparation of active caspase-3 in E. coli by designing its thrombin-activatable precursors
Hyo Jin Kang, Young-mi Lee, Yu-Jin Jeong, Kyoungsook Park, Mi Jang, Sung Goo Park, Kwang-Hee Bae, Moonil Kim, Sang J Chung
BMC Biotechnology 2008, 8:92 (11December2008)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Methodology article
An efficient one-step site-directed deletion, insertion, single and multiple-site plasmid mutagenesis protocol
Huanting Liu, James H Naismith
BMC Biotechnology 2008, 8:91 (4December2008)
[Abstract] [Full text] [PDF] [PubMed] [Related articles] [Cited on BioMed Central]

Methodology article
Reliable microRNA profiling in routinely processed formalin-fixed paraffin-embedded breast cancer specimens using fluorescence labelled bead technology
Britta Hasemeier, Matthias Christgen, Hans Kreipe, Ulrich Lehmann
BMC Biotechnology 2008, 8:90 (27November2008)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Preparation of alginate coated chitosan microparticles for vaccine delivery
XingYi Li, XiangYe Kong, Shuai Shi, XiuLing Zheng, Gang Guo, YuQuan Wei, ZhiYong Qian
BMC Biotechnology 2008, 8:89 (19November2008)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Methodology article
Homologous recombination-mediated cloning and manipulation of genomic DNA regions using Gateway and recombineering systems
Kevin Rozwadowski, Wen Yang, Sateesh Kagale
BMC Biotechnology 2008, 8:88 (17November2008)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
A novel expression platform for the production of diabetes-associated autoantigen human glutamic acid decarboxylase (hGAD65)
Xiaofeng Wang, Martin Brandsma, Reynald Tremblay, Denis Maxwell, Anthony M Jevnikar, Norm Huner, Shengwu Ma
BMC Biotechnology 2008, 8:87 (17November2008)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Methodology article
Development of a novel ozone- and photo-stable HyPer5 red fluorescent dye for array CGH and microarray gene expression analysis with consistent performance irrespective of environmental conditions
Mubasher Dar, Theresa Giesler, Rob Richardson, Christine Cai, Mike Cooper, Shahin Lavasani, Peter Kille, Thierry Voet, Joris Vermeesch
BMC Biotechnology 2008, 8:86 (12November2008)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Generation of potent neutralizing human monoclonal antibodies against cytomegalovirus infection from immune B cells
Ada Funaro, Giorgio Gribaudo, Anna Luganini, Erika Ortolan, Nicola Lo Buono, Elisa Vicenzi, Luca Cassetta, Santo Landolfo, Richard Buick, Luca Falciola, Marianne Murphy, Gianni Garotta, Fabio Malavasi
BMC Biotechnology 2008, 8:85 (12November2008)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Differential solubility of curcuminoids in serum and albumin solutions: implications for analytical and therapeutic applications
Wolfgang W Quitschke
BMC Biotechnology 2008, 8:84 (6November2008)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Induction of multiple pleiotropic drug resistance genes in yeast engineered to produce an increased level of anti-malarial drug precursor, artemisinic acid
Dae-Kyun Ro, Mario Ouellet, Eric M Paradise, Helcio Burd, Diana Eng, Chris J Paddon, Jack D Newman, Jay D Keasling
BMC Biotechnology 2008, 8:83 (4November2008)
[Abstract] [Full text] [PDF] [PubMed] [Related articles] [Cited on BioMed Central]

Methodology article
Recombinant amyloid beta-peptide production by coexpression with an affibody ligand
Bertil Macao, Wolfgang Hoyer, Anders Sandberg, Ann-Christin Brorsson, Christopher M Dobson, Torleif Hrd
BMC Biotechnology 2008, 8:82 (30October2008)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Methodology article
Application of the dual-luciferase reporter assay to the analysis of promoter activity in Zebrafish embryos
Francisca Alcaraz-Prez, Victoriano Mulero, Mara L Cayuela
BMC Biotechnology 2008, 8:81 (27October2008)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Isolation and characterization of a T7-like lytic phage for Pseudomonas fluorescens
Sanna Sillankorva, Peter Neubauer, Joana Azeredo
BMC Biotechnology 2008, 8:80 (27October2008)
[Abstract] [Full text] [PDF] [PubMed] [Related articles] [Cited on BioMed Central]

Research article
Pseudomonas fluorescens biofilms subjected to phage phiIBB-PF7A
Sanna Sillankorva, Peter Neubauer, Joana Azeredo
BMC Biotechnology 2008, 8:79 (27October2008)
[Abstract] [Full text] [PDF] [PubMed] [Related articles] [Cited on BioMed Central]

Research article
Development of a strategy to functionalize a dextrin-based hydrogel for animal cell cultures using a starch-binding module fused to RGD sequence
Susana M Moreira, Fbia K Andrade, Lucla Domingues, Miguel Gama
BMC Biotechnology 2008, 8:78 (14October2008)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Methodology article
A construct with fluorescent indicators for conditional expression of miRNA
Linghua Qiu, Hongyan Wang, Xugang Xia, Hongxia Zhou, Zuoshang Xu
BMC Biotechnology 2008, 8:77 (7October2008)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Methodology article
One-step refolding and purification of disulfide-containing proteins with a C-terminal MESNA thioester
Maartje MC Bastings, Ingrid van Baal, EW Meijer, Maarten Merkx
BMC Biotechnology 2008, 8:76 (1October2008)
[Abstract] [Full text] [PDF] [PubMed] [Related articles] [Cited on BioMed Central]

Research article
Pharmaceutical induction of ApoE secretion by multipotent mesenchymal stromal cells (MSCs)
Suzanne Zeitouni, Brian S Ford, Sean M Harris, Mandolin J Whitney, Carl A Gregory, Darwin J Prockop
BMC Biotechnology 2008, 8:75 (29September2008)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Methodology article
Inhibition of cell growth and invasion by epidermal growth factor-targeted phagemid particles carrying siRNA against focal adhesion kinase in the presence of hydroxycamptothecin
Xiu-Mei Cai, Hai-Long Xie, Ming-Zhu Liu, Xi-Liang Zha
BMC Biotechnology 2008, 8:74 (18September2008)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Shewanella oneidensis: a new and efficient System for Expression and Maturation of heterologous [Fe-Fe] Hydrogenase from Chlamydomonas reinhardtii
Kateryna Sybirna, Tatiana Antoine, Pia Lindberg, Vincent Fourmond, Marc Rousset, Vincent Mjean, Herv Bottin
BMC Biotechnology 2008, 8:73 (18September2008)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
The stabilizing effects of immobilization in D-amino acid oxidase from Trigonopsis variabilis
Iskandar Dib, Bernd Nidetzky
BMC Biotechnology 2008, 8:72 (17September2008)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Methodology article
Molecular analysis of chondrocytes cultured in agarose in response to dynamic compression
Carole Bougault, Anne Paumier, Elisabeth Aubert-Foucher, Frdric Mallein-Gerin
BMC Biotechnology 2008, 8:71 (15September2008)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Allogeneic and autogenous transplantations of MSCs in treatment of the physeal bone bridge in rabbits
Ladislav Planka, Petr Gal, Helga Kecova, Jiri Klima, Jana Hlucilova, Eva Filova, Evzen Amler, Petr Krupa, Leos Kren, Robert Srnec, Lucie Urbanova, Jana Lorenzova, Alois Necas
BMC Biotechnology 2008, 8:70 (12September2008)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Measuring microRNAs: Comparisons of microarray and quantitative PCR measurements, and of different total RNA prep methods
Robert A Ach, Hui Wang, Bo Curry
BMC Biotechnology 2008, 8:69 (11September2008)
[Abstract] [Full text] [PDF] [PubMed] [Related articles] [Cited on BioMed Central]

Research article
Generation and characterization of a human single-chain fragment variable (scFv) antibody against cytosine deaminase from Yeast
Alessandra Mallano, Silvia Zamboni, Giulia Carpinelli, Filippo Santoro, Michela Flego, Alessandro Ascione, Mara Gellini, Marina Tombesi, Franca Podo, Maurizio Cianfriglia
BMC Biotechnology 2008, 8:68 (10September2008)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Phosphoribosyl pyrophosphate synthetase activity affects growth and riboflavin production in Ashbya gossypii
Alberto Jimnez, Mara A Santos, Jos L Revuelta
BMC Biotechnology 2008, 8:67 (9September2008)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Development of human antibody fragments using antibody phage display for the detection and diagnosis of Venezuelan equine encephalitis virus (VEEV)
Martina Inga Kirsch, Birgit Hlseweh, Christoph Nacke, Torsten Rlker, Thomas Schirrmann, Hans-Jrgen Marschall, Michael Hust, Stefan Dbel
BMC Biotechnology 2008, 8:66 (2September2008)
[Abstract] [Full text] [PDF] [PubMed] [Related articles] [Cited on BioMed Central]

Research article
Stable high volumetric production of glycosylated human recombinant IFNalpha2b in HEK293 cells
Martin Loignon, Sylvie Perret, John Kelly, Denise Boulais, Brian Cass, Louis Bisson, Fatemeh Afkhamizarreh, Yves Durocher
BMC Biotechnology 2008, 8:65 (27August2008)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Methodology article
Display of a Maize cDNA library on baculovirus infected insect cells
Helene Y Meller Harel, Veronique Fontaine, Hongying Chen, Ian M Jones, Paul A Millner
BMC Biotechnology 2008, 8:64 (12August2008)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Methodology article
Dual-In/Out strategy for genes integration into bacterial chromosome: a novel approach to step-by-step construction of plasmid-less marker-less recombinant E. coli strains with predesigned genome structure
Natalia I Minaeva, Evgeny R Gak, Danila V Zimenkov, Aleksandra Yu Skorokhodova, Irina V Biryukova, Sergey V Mashko
BMC Biotechnology 2008, 8:63 (12August2008)
[Abstract] [Full text] [PDF] [PubMed] [Related articles] [Cited on BioMed Central]

Methodology article
Comparison of the mismatch-specific endonuclease method and denaturing high-performance liquid chromatography for the identification of HBB gene mutations
Chia-Cheng Hung, Yi-Ning Su, Chia-Yun Lin, Yin-Fei Chang, Chien-Hui Chang, Wen-Fang Cheng, Chi-An Chen, Chien-Nan Lee, Win-Li Lin
BMC Biotechnology 2008, 8:62 (12August2008)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Plasmid selection in Escherichia coli using an endogenous essential gene marker
Shan Goh, Liam Good
BMC Biotechnology 2008, 8:61 (11August2008)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Genomic insertion of lentiviral DNA circles directed by the yeast Flp recombinase
Brian Moldt, Nicklas H Staunstrup, Maria Jakobsen, Rafael J Yez-Muoz, Jacob G Mikkelsen
BMC Biotechnology 2008, 8:60 (9August2008)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Methodology article
Detection of N-acyl homoserine lactones using a traI-luxCDABE-based biosensor as a high-throughput screening tool
Steve P Bernier, Anne L Beeston, Pamela A Sokol
BMC Biotechnology 2008, 8:59 (30July2008)
[Abstract] [Full text] [PDF] [PubMed] [Related articles] [Cited on BioMed Central]

Research article
A comparative study of protein synthesis in in vitro systems: from the prokaryotic reconstituted to the eukaryotic extract-based
Jason R Hillebrecht, Shaorong Chong
BMC Biotechnology 2008, 8:58 (29July2008)
[Abstract] [Full text] [PDF] [PubMed] [Related articles] [Cited on BioMed Central]

Research article
Critical evaluation of HPV16 gene copy number quantification by SYBR green PCR
Ian Roberts, Grace Ng, Nicola Foster, Margaret Stanley, Michael T Herdman, Mark R Pett, Andrew Teschendorff, Nicholas Coleman
BMC Biotechnology 2008, 8:57 (24July2008)
[Abstract] [Full text] [PDF] [PubMed] [Related articles] [1 comment]

Research article
Successful elimination of non-neural cells and unachievable elimination of glial cells by means of commonly used cell culture manipulations during differentiation of GFAP and SOX2 positive neural progenitors (NHA) to neuronal cells
Monika Witusik, Sylwester Piaskowski, Krystyna Hulas-Bigoszewska, Magdalena Zakrzewska, Sylwia M Gresner, S Ausim Azizi, Barbara Krynska, Pawel P Liberski, Piotr Rieske
BMC Biotechnology 2008, 8:56 (19July2008)
[Abstract] [Full text] [PDF] [PubMed] [Related articles] [Cited on BioMed Central]

Methodology article
Analysis of transient phosphorylation-dependent protein-protein interactions in living mammalian cells using split-TEV
Michael C Wehr, Lisa Reinecke, Anna Botvinnik, Moritz J Rossner
BMC Biotechnology 2008, 8:55 (13July2008)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Three-dimensional culture of human meniscal cells: Extracellular matrix and proteoglycan production
Helen E Gruber, David Mauerhan, Yin Chow, Jane A Ingram, H James Norton, Edward N Hanley Jr, Yubo Sun
BMC Biotechnology 2008, 8:54 (26June2008)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Expression of HIV-1 antigens in plants as potential subunit vaccines
Ann Meyers, Ereck Chakauya, Enid Shephard, Fiona L Tanzer, James Maclean, Alisson Lynch, Anna-Lise Williamson, Edward P Rybicki
BMC Biotechnology 2008, 8:53 (23June2008)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Methodology article
Human IL-12 p40 as a reporter gene for high-throughput screening of engineered mouse embryonic stem cells
Leonardo D'Aiuto, Clinton S Robison, Margherita Gigante, Edward Nwanegbo, Benjamin Shaffer, Meena Sukhwani, Carlos A Castro, J Richard Chaillet
BMC Biotechnology 2008, 8:52 (3June2008)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Methodology article
Construction of a series of vectors for high throughput cloning and expression screening of membrane proteins from Mycobacterium tuberculosis
Huajun Qin, Jian Hu, Yuanzhi Hua, Shridhar V Challa, Timothy A Cross, Fei P Gao
BMC Biotechnology 2008, 8:51 (16May2008)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Substantially improved pharmacokinetics of recombinant human butyrylcholinesterase by fusion to human serum albumin
Yue-Jin Huang, Paul M Lundy, Anthoula Lazaris, Yue Huang, Hernan Baldassarre, Bin Wang, Carl Turcotte, Mlanie Ct, Annie Bellemare, Annie S Bilodeau, Sandra Brouillard, Madjid Touati, Peter Herskovits, Isabelle Bgin, Nathalie Neveu, Eric Brochu, Janice Pierson, Duncan K Hockley, Douglas M Cerasoli, David E Lenz, Harvey Wilgus, Costas N Karatzas, Solomon Langermann
BMC Biotechnology 2008, 8:50 (16May2008)
[Abstract] [Full text] [PDF] [PubMed] [Related articles] [Cited on BioMed Central]

Research article
Viral vectors based on bidirectional cell-specific mammalian promoters and transcriptional amplification strategy for use in vitro and in vivo
Beihui Liu, Julian F Paton, Sergey Kasparov
BMC Biotechnology 2008, 8:49 (16May2008)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Impact of point-mutations on the hybridization affinity of surface-bound DNA/DNA and RNA/DNA oligonucleotide-duplexes: Comparison of single base mismatches and base bulges
Thomas Naiser, Oliver Ehler, Jona Kayser, Timo Mai, Wolfgang Michel, Albrecht Ott
BMC Biotechnology 2008, 8:48 (13May2008)
[Abstract] [Full text] [PDF] [PubMed] [Related articles] [Cited on BioMed Central]

Methodology article
A kinetic-based sigmoidal model for the polymerase chain reaction and its application to high-capacity absolute quantitative real-time PCR
Robert G Rutledge, Don Stewart
BMC Biotechnology 2008, 8:47 (8May2008)
[Abstract] [Full text] [PDF] [PubMed] [Related articles] [Cited on BioMed Central] [1 comment]

Research article
Hydroxycamptothecin-loaded nanoparticles enhance target drug delivery and anticancer effect
Anxun Wang, Su Li
BMC Biotechnology 2008, 8:46 (4May2008)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Methodology article
Flow cytometric monitoring of influenza A virus infection in MDCK cells during vaccine production
Josef Schulze-Horsel, Yvonne Genzel, Udo Reichl
BMC Biotechnology 2008, 8:45 (30April2008)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Methodology article
PCR-based gene synthesis to produce recombinant proteins for crystallization
Damien Marsic, Ronny C Hughes, Miranda L Byrne-Steele, Joseph D Ng
BMC Biotechnology 2008, 8:44 (29April2008)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Characterization of a novel non-specific nuclease from thermophilic bacteriophage GBSV1
Qing Song, Xiaobo Zhang
BMC Biotechnology 2008, 8:43 (28April2008)
[Abstract] [Full text] [PDF] [PubMed] [Related articles] [Cited on BioMed Central]

Research article
Differential protein expression following low temperature culture of suspension CHO-K1 cells
Niraj Kumar, Patrick Gammell, Paula Meleady, Michael Henry, Martin Clynes
BMC Biotechnology 2008, 8:42 (22April2008)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
In vivo site-specific biotinylation of proteins within the secretory pathway using a single vector system
Andrea Predonzani, Francesca Arnoldi, Alejandro Lpez-Requena, Oscar R Burrone
BMC Biotechnology 2008, 8:41 (18April2008)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Methodology article
Simultaneous monitoring of independent gene expression patterns in two types of cocultured fibroblasts with different color-emitting luciferases
Takako Noguchi, Masaaki Ikeda, Yoshihiro Ohmiya, Yoshihiro Nakajima
BMC Biotechnology 2008, 8:40 (17April2008)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Electrospun micro- and nanofiber tubes for functional nervous regeneration in sciatic nerve transections
Silvia Panseri, Carla Cunha, Joseph Lowery, Ubaldo Del Carro, Francesca Taraballi, Stefano Amadio, Angelo Vescovi, Fabrizio Gelain
BMC Biotechnology 2008, 8:39 (11April2008)
[Abstract] [Full text] [PDF] [PubMed] [Related articles] [Cited on BioMed Central]

Methodology article
Comparison of the developmental potential of 2-week-old preantral follicles derived from vitrified ovarian tissue slices, vitrified whole ovaries and vitrified/transplanted newborn mouse ovaries using the metal surface method
Ta-Chin Lin, Jui-Mei Yen, Tsung-Cheng Kuo, Kun-Bing Gong, Kung-Hao Hsu, Teng-Tsao Hsu
BMC Biotechnology 2008, 8:38 (4April2008)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Killing cancer cells by targeted drug-carrying phage nanomedicines
Hagit Bar, Iftach Yacoby, Itai Benhar
BMC Biotechnology 2008, 8:37 (3April2008)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Development of patatin knockdown potato tubers using RNA interference (RNAi) technology, for the production of human-therapeutic glycoproteins
Yoon-Sik Kim, Yong-Hwa Lee, Hyun-Soon Kim, Mi-Sun Kim, Kyu-Woong Hahn, Jeong-Heon Ko, Hyouk Joung, Jae-Heung Jeon
BMC Biotechnology 2008, 8:36 (3April2008)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Nanopolymers improve delivery of exon skipping oligonucleotides and concomitant dystrophin expression in skeletal muscle of mdx mice
Jason H Williams, Rebecca C Schray, Shashank R Sirsi, Gordon J Lutz
BMC Biotechnology 2008, 8:35 (2April2008)
[Abstract] [Full text] [PDF] [PubMed] [Related articles] [Cited on BioMed Central]

Research article
Identification of a GTP-bound Rho specific scFv molecular sensor by phage display selection
Marine Goffinet, Patrick Chinestra, Isabelle Lajoie-Mazenc, Claire Medale-Giamarchi, Gilles Favre, Jean-Charles Faye
BMC Biotechnology 2008, 8:34 (31March2008)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Methodology article
Improved production of human type II procollagen in the yeast Pichia pastoris in shake flasks by a wireless-controlled fed-batch system
Maria Ruottinen, Monika Bollok, Martin Kgler, Antje Neubauer, Mirja Krause, Eija-Riitta Hmlinen, Johanna Myllyharju, Antti Vasala, Peter Neubauer
BMC Biotechnology 2008, 8:33 (27March2008)
[Abstract] [Full text] [PDF] [PubMed] [Related articles] [Cited on BioMed Central]

Methodology article
A set of ligation-independent in vitro translation vectors for eukaryotic protein production
Viola Bardczy, Viktria Gczi, Tatsuya Sawasaki, Yaeta Endo, Tams Mszros
BMC Biotechnology 2008, 8:32 (27March2008)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Methodology article
Gene targeting in adult rhesus macaque fibroblasts
Daniel T Meehan, Mary Ann Zink, Melissa Mahlen, Marilu Nelson, Warren G Sanger, Shoukhrat M Mitalipov, Don P Wolf, Michel M Ouellette, Robert B Norgren Jr
BMC Biotechnology 2008, 8:31 (26March2008)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Methodology article
RNAi-mediated gene silencing in tick synganglia: A proof of concept study
Shahid Karim, Bronwyn Kenny, Emily Troiano, Thomas N Mather
BMC Biotechnology 2008, 8:30 (26March2008)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Production of functionally active Penicillium chrysogenum isopenicillin N synthase in the yeast Hansenula polymorpha
Loknath Gidijala, Roel AL Bovenberg, Paul Klaassen, Ida J van der Klei, Marten Veenhuis, Jan AKW Kiel
BMC Biotechnology 2008, 8:29 (19March2008)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Design of a zinc finger protein binding a sequence upstream of the A20 gene
Yong Wei, Dajun Ying, Chunli Hou, Xiaoping Cui, Chuhong Zhu
BMC Biotechnology 2008, 8:28 (19March2008)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Methodology article
Isolation of soybean protein P34 from oil bodies using hydrophobic interaction chromatography
Eva Sewekow, Lars Christian Kessler, Andreas Seidel-Morgenstern, Hermann-Josef Rothktter
BMC Biotechnology 2008, 8:27 (11March2008)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Methodology article
Comparison of different real-time PCR chemistries and their suitability for detection and quantification of genetically modified organisms
Meti Buh Gapari, Katarina Cankar, Jana el, Kristina Gruden
BMC Biotechnology 2008, 8:26 (6March2008)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Glycosyltransferase efficiently controls phenylpropanoid pathway
Anna Aksamit-Stachurska, Alina Korobczak-Sosna, Anna Kulma, Jan Szopa
BMC Biotechnology 2008, 8:25 (5March2008)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Intracellular delivery of peptides via association with ubiquitin or SUMO-1 coupled to protein transduction domains
Anne-Laure Vitte, Pierre Jalinot
BMC Biotechnology 2008, 8:24 (29February2008)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Effects of size and topology of DNA molecules on intracellular delivery with non-viral gene carriers
Charlie Yu Ming Hsu, Hasan Uluda
BMC Biotechnology 2008, 8:23 (29February2008)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Methodology article
Protein kinase substrate identification on functional protein arrays
Lihao Meng, Gregory A Michaud, Janie S Merkel, Fang Zhou, Jing Huang, Dawn R Mattoon, Barry Schweitzer
BMC Biotechnology 2008, 8:22 (28February2008)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Systolic and diastolic ventricular function in zebrafish embryos: Influence of norepenephrine, MS-222 and temperature
Martin A Denvir, Carl S Tucker, John J Mullins
BMC Biotechnology 2008, 8:21 (27February2008)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Effect of ionic strength and presence of serum on lipoplexes structure monitorized by FRET
Catarina Madeira, Lus MS Loura, Manuel Prieto, Aleksander Fedorov, M Raquel Aires-Barros
BMC Biotechnology 2008, 8:20 (26February2008)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Methodology article
Enhanced gene expression from retroviral vectors
Magnus Bl, David R Micklem, James B Lorens
BMC Biotechnology 2008, 8:19 (25February2008)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Methodology article
Quantification of Cre-mediated recombination by a novel strategy reveals a stable extra-chromosomal deletion-circle in mice
Wouter N Leonhard, Jeroen H Roelfsema, Irma S Lantinga-van Leeuwen, Martijn H Breuning, Dorien JM Peters
BMC Biotechnology 2008, 8:18 (25February2008)
[Abstract] [Full text] [PDF] [PubMed] [Related articles] [Cited on BioMed Central]

Methodology article
Amplification of multiple genomic loci from single cells isolated by laser micro-dissection of tissues
Dan Frumkin, Adam Wasserstrom, Shalev Itzkovitz, Alon Harmelin, Gideon Rechavi, Ehud Shapiro
BMC Biotechnology 2008, 8:17 (20February2008)
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Research article
Simplified approaches for the development of an ELISA to detect circulating autoantibodies to p53 in cancer patients
Ratchada Cressey, Saranya Pimpa, Busyamas Chewaskulyong, Nirush Lertprasertsuke, Somchareon Saeteng, Chatchai Tayapiwatana, Watchara Kasinrerk
BMC Biotechnology 2008, 8:16 (20February2008)
[Abstract] [Full text] [PDF] [PubMed] [Related articles] [Cited on BioMed Central]

Research article
Solubility enhancement of aggregation-prone heterologous proteins by fusion expression using stress-responsive Escherichia coli protein, RpoS
Jin-Seung Park, Kyung-Yeon Han, Jong-Ho Lee, Jong-Am Song, Keum-Young Ahn, Hyuk-Seong Seo, Sang-Jun Sim, Seung-Wook Kim, Jeewon Lee
BMC Biotechnology 2008, 8:15 (19February2008)
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Expression of recombinant Rhipicephalus (Boophilus) microplus, R. annulatus and R. decoloratus Bm86 orthologs as secreted proteins in Pichia pastoris
Mario Canales, Jos M Prez de la Lastra, Victoria Naranjo, Ard M Nijhof, Michelle Hope, Frans Jongejan, Jos de la Fuente
BMC Biotechnology 2008, 8:14 (14February2008)
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Efficient and reproducible generation of high-expressing, stable human cell lines without need for antibiotic selection
Gudrun Schiedner, Sabine Hertel, Corinna Bialek, Helmut Kewes, Gero Waschtza, Christoph Volpers
BMC Biotechnology 2008, 8:13 (12February2008)
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Methodology article
High-throughput avian molecular sexing by SYBR green-based real-time PCR combined with melting curve analysis
Hsueh-Wei Chang, Chun-An Cheng, De-Leung Gu, Chia-Che Chang, San-Hua Su, Cheng-Hao Wen, Yii-Cheng Chou, Ta-Ching Chou, Cheng-Te Yao, Chi-Li Tsai, Chien-Chung Cheng
BMC Biotechnology 2008, 8:12 (12February2008)
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Methodology article
Quantification of PRL/Stat5 signaling with a novel pGL4-CISH reporter
Feng Fang, Giovanni Antico, Jiamao Zheng, Charles V Clevenger
BMC Biotechnology 2008, 8:11 (6February2008)
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Improved RNA quality and TaqMan Pre-amplification method (PreAmp) to enhance expression analysis from formalin fixed paraffin embedded (FFPE) materials
Jinghuan Li, Paul Smyth, Susanne Cahill, Karen Denning, Richard Flavin, Sinead Aherne, Marco Pirotta, Simone M Guenther, John J O'Leary, Orla Sheils
BMC Biotechnology 2008, 8:10 (6February2008)
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Research article
Recombinant gas vesicles from Halobacterium sp. displaying SIV peptides demonstrate biotechnology potential as a pathogen peptide delivery vehicle
Marinko Sremac, Elizabeth S Stuart
BMC Biotechnology 2008, 8:9 (31January2008)
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Research article
Spherezymes: A novel structured self-immobilisation enzyme technology
Dean Brady, Justin Jordaan, Clinton Simpson, Avashnee Chetty, Cherise Arumugam, Francis S Moolman
BMC Biotechnology 2008, 8:8 (31January2008)
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Research article
Laser surgery of zebrafish (Danio rerio) embryos using femtosecond laser pulses: Optimal parameters for exogenous material delivery, and the laser's effect on short- and long-term development
Vikram Kohli, Abdulhakem Y Elezzabi
BMC Biotechnology 2008, 8:7 (29January2008)
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Methodology article
Negative selection of chronic lymphocytic leukaemia cells using a bifunctional rosette-based antibody cocktail
Salim Essakali, Dennis Carney, David Westerman, Peter Gambell, John F Seymour, Alexander Dobrovic
BMC Biotechnology 2008, 8:6 (29January2008)
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Generation of functional scFv intrabody to abate the expression of CD147 surface molecule of 293A cells
Khajornsak Tragoolpua, Nutjeera Intasai, Watchara Kasinrerk, Sabine Mai, Yuan Yuan, Chatchai Tayapiwatana
BMC Biotechnology 2008, 8:5 (29January2008)
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Research article
Efficient display of active lipase LipB52 with a Pichia pastoris cell surface display system and comparison with the LipB52 displayed on Saccharomyces cerevisiae cell surface
Zhengbing Jiang, Bei Gao, Ren Ren, Xingyi Tao, Yushu Ma, Dongzhi Wei
BMC Biotechnology 2008, 8:4 (28January2008)
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Research article
Enhancement of hybridoma formation, clonability and cell proliferation in a nanoparticle-doped aqueous environment
Natalie Gavrilov-Yusim, Ekaterina Hahiashvili, Marina Tashker, Victoria Yavelsky, Ohad Karnieli, Leslie Lobel
BMC Biotechnology 2008, 8:3 (14January2008)
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Research article
Optimization of an E. coli L-rhamnose-inducible expression vector: test of various genetic module combinations
Angelika Wegerer, Tianqi Sun, Josef Altenbuchner
BMC Biotechnology 2008, 8:2 (14January2008)
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Research article
Quantification of the CBD-FITC conjugates surface coating on cellulose fibres
Ricardo Pinto, Antnio L Amaral, Eugnio C Ferreira, Manuel Mota, Manuel Vilanova, Katia Ruel, Miguel Gama
BMC Biotechnology 2008, 8:1 (9January2008)
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