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Editor-in-Chief
Melissa Norton, MD

Biology Editor
Penelope Webb, PhD

In-house Editor
J. Ann Le Good




BMC Biotechnology, Volume 7

 

 

 

Methodology article
Production of highly knotted DNA by means of cosmid circularization inside phage capsids
Sonia Trigueros, Joaquim Roca
BMC Biotechnology 2007, 7:94 (21December2007)
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Research article
Improved functional expression of recombinant human ether-a-go-go (hERG) K+ channels by cultivation at reduced temperature
Mao Xiang Chen, Shaun L Sandow, Virginie Doceul, Yu Hua Chen, Heather Harper, Bruce Hamilton, Helen J Meadows, Derek J Trezise, Jeff J Clare
BMC Biotechnology 2007, 7:93 (20December2007)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Quantitative comparison of DNA detection by GFP-lac repressor tagging, fluorescence in situ hybridization and immunostaining
Il-Han Kim, Jens Nagel, Simone Otten, Boris Knerr, Roland Eils, Karl Rohr, Steffen Dietzel
BMC Biotechnology 2007, 7:92 (20December2007)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Methodology article
Microarray-based method for detection of unknown genetic modifications
Torstein Tengs, Anja B Kristoffersen, Knut G Berdal, Tage Thorstensen, Melinka A Butenko, Hvard Nesvold, Arne Holst-Jensen
BMC Biotechnology 2007, 7:91 (18December2007)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Plasmid-based genetic modification of human bone marrow-derived stromal cells: analysis of cell survival and transgene expression after transplantation in rat spinal cord
Mark W Ronsyn, Jasmijn Daans, Gie Spaepen, Shyama Chatterjee, Katrien Vermeulen, Patrick D'Haese, Viggo FI Van Tendeloo, Eric Van Marck, Dirk Ysebaert, Zwi N Berneman, Philippe G Jorens, Peter Ponsaerts
BMC Biotechnology 2007, 7:90 (14December2007)
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Research article
GADD153 expression does not necessarily correlate with changes in culture behavior of hybridoma cells
Matthew Mallory, Kevin Chartrand, Eric R Gauthier
BMC Biotechnology 2007, 7:89 (10December2007)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Self-assembling Fmoc dipeptide hydrogel for in situ 3D cell culturing
Thomas Liebmann, Susanna Rydholm, Victor Akpe, Hjalmar Brismar
BMC Biotechnology 2007, 7:88 (10December2007)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Methodology article
Single-cell duplex RT-LATE-PCR reveals Oct4 and Xist RNA gradients in 8-cell embryos
Cristina Hartshorn, Judith J Eckert, Odelya Hartung, Lawrence J Wangh
BMC Biotechnology 2007, 7:87 (7December2007)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Arginine-to-lysine substitutions influence recombinant horseradish peroxidase stability and immobilisation effectiveness
Barry J Ryan, Ciarn 'Fgin
BMC Biotechnology 2007, 7:86 (5December2007)
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Methodology article
A new generation of pPRIG-based retroviral vectors
Olivier Albagli-Curiel, Yann Lcluse, Philippe Pognonec, Kim E Boulukos, Patrick Martin
BMC Biotechnology 2007, 7:85 (30November2007)
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Research article
Double knockdown of 1,6-fucosyltransferase (FUT8) and GDP-mannose 4,6-dehydratase (GMD) in antibody-producing cells: a new strategy for generating fully non-fucosylated therapeutic antibodies with enhanced ADCC
Harue Imai-Nishiya, Katsuhiro Mori, Miho Inoue, Masako Wakitani, Shigeru Iida, Kenya Shitara, Mitsuo Satoh
BMC Biotechnology 2007, 7:84 (30November2007)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Enhanced transduction of colonic cell lines in vitro and the inflamed colon in mice by viral vectors, derived from adeno-associated virus serotype 2, using virus-microbead conjugates bearing lectin
Samuel J Farlow, Alan Jerusalmi, Takeshi Sano
BMC Biotechnology 2007, 7:83 (28November2007)
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Methodology article
Compatible solutes from hyperthermophiles improve the quality of DNA microarrays
Nicoletta Mascellani, Xiuping Liu, Simona Rossi, Jlenia Marchesini, Davide Valentini, Diego Arcelli, Cristian Taccioli, Mauro Helmer Citterich, Chang-Gong Liu, Rita Evangelisti, Giandomenico Russo, Jorge M Santos, Carlo M Croce, Stefano Volinia
BMC Biotechnology 2007, 7:82 (23November2007)
[Abstract] [Full text] [PDF] [PubMed] [Related articles] [Cited on BioMed Central]

Research article
A focused antibody library for selecting scFvs expressed at high levels in the cytoplasm
Pascal Philibert, Audrey Stoessel, Wei Wang, Annie-Paule Sibler, Nicole Bec, Christian Larroque, Jeffery G Saven, Jrme Courtte, Etienne Weiss, Pierre Martineau
BMC Biotechnology 2007, 7:81 (22November2007)
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Research article
Construction of stably maintained non-mobilizable derivatives of RSF1010 lacking all known elements essential for mobilization
Joanna I Katashkina, Tatiana M Kuvaeva, Irina G Andreeva, Alexandra Yu Skorokhodova, Irina V Biryukova, Irina L Tokmakova, Lubov I Golubeva, Sergey V Mashko
BMC Biotechnology 2007, 7:80 (21November2007)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Comparison of chicken 7SK and U6 RNA polymerase III promoters for short hairpin RNA expression
Stephanie C Bannister, Terry G Wise, David M Cahill, Timothy J Doran
BMC Biotechnology 2007, 7:79 (19November2007)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Isolation of anti-toxin single domain antibodies from a semi-synthetic spiny dogfish shark display library
Jinny L Liu, George P Anderson, Ellen R Goldman
BMC Biotechnology 2007, 7:78 (19November2007)
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Methodology article
Revealing biases inherent in recombination protocols
Javier F Chaparro-Riggers, Bernard LW Loo, Karen M Polizzi, Phillip R Gibbs, Xiao-Song Tang, Mark J Nelson, Andreas S Bommarius
BMC Biotechnology 2007, 7:77 (14November2007)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Characterization of EvaGreen and the implication of its physicochemical properties for qPCR applications
Fei Mao, Wai-Yee Leung, Xing Xin
BMC Biotechnology 2007, 7:76 (9November2007)
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Research article
Adeno-associated viral vectors engineered for macrolide-adjustable transgene expression In mammalian cells and mice
David A Fluri, Marie Daoud-El Baba, Martin Fussenegger
BMC Biotechnology 2007, 7:75 (6November2007)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Methodology article
Long term expression of bicistronic vector driven by the FGF-1 IRES in mouse muscle
Camille Allera-Moreau, Aurlie Delluc-Clavires, Caroline Castano, Loc Van den Berghe, Muriel Golzio, Marc Moreau, Justin Teissi, Jean-Franois Arnal, Anne-Catherine Prats
BMC Biotechnology 2007, 7:74 (28October2007)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Software
Webtag: a new web tool providing tags/anchors for RT-PCR experiments with prokaryotes
Fernando Lopes Pinto, Hkan Svensson, Peter Lindblad
BMC Biotechnology 2007, 7:73 (25October2007)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
An integrative expression vector for Actinosynnema pretiosum
Shan Goh, Andrea Camattari, Daniel Ng, Ruth Song, Kevin Madden, Janet Westpheling, Victor VT Wong
BMC Biotechnology 2007, 7:72 (24October2007)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Enhancement of cell proliferation in various mammalian cell lines by gene insertion of a cyclin-dependent kinase homolog
Pratik Jaluria, Michael Betenbaugh, Konstantinos Konstantopoulos, Joseph Shiloach
BMC Biotechnology 2007, 7:71 (18October2007)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Tumor-infiltrating B lymphocytes as an efficient source of highly specific immunoglobulins recognizing tumor cells
Emiliano Pavoni, Giorgia Monteri, Daniela Santapaola, Fiorella Petronzelli, Anna Maria Anastasi, Angela Pelliccia, Valeria D'Alessio, Rita De Santis, Olga Minenkova
BMC Biotechnology 2007, 7:70 (18October2007)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Methodology article
In situ detection of non-polyadenylated RNA molecules using Turtle Probes and target primed rolling circle PRINS
Magnus Stougaard, Jakob S Lohmann, Magdalena Zajac, Stephen Hamilton-Dutoit, Jrn Koch
BMC Biotechnology 2007, 7:69 (18October2007)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Establishment of a Bovine Herpesvirus 4 based vector expressing a secreted form of the Bovine Viral Diarrhoea Virus structural glycoprotein E2 for immunization purposes
Gaetano Donofrio, Chiara Sartori, Lara Ravanetti, Sandro Cavirani, Laurent Gillet, Alain Vanderplasschen, Simone Taddei, Cesidio Filippo Flammini
BMC Biotechnology 2007, 7:68 (18October2007)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Quantum dot imaging for embryonic stem cells
Shuan Lin, Xiaoyan Xie, Manishkumar R Patel, Yao-Hung Yang, Zongjin Li, Feng Cao, Oliver Gheysens, Yan Zhang, Sanjiv S Gambhir, Jiang Hong Rao, Joseph C Wu
BMC Biotechnology 2007, 7:67 (9October2007)
[Abstract] [Full text] [PDF] [PubMed] [Related articles] [Cited on BioMed Central]

Research article
High content image analysis for human H4 neuroglioma cells exposed to CuO nanoparticles
Fuhai Li, Xiaobo Zhou, Jinmin Zhu, Jinwen Ma, Xudong Huang, Stephen TC Wong
BMC Biotechnology 2007, 7:66 (9October2007)
[Abstract] [Full text] [PDF] [PubMed] [Related articles] [Cited on BioMed Central]

Methodology article
Trinucleotide cassettes increase diversity of T7 phage-displayed peptide library
Lauren RH Krumpe, Kathryn M Schumacher, James B McMahon, Lee Makowski, Toshiyuki Mori
BMC Biotechnology 2007, 7:65 (5October2007)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
A systematic approach for testing expression of human full-length proteins in cell-free expression systems
Claudia Langlais, Birgit Guilleaume, Nadja Wermke, Tina Scheuermann, Lars Ebert, Joshua LaBaer, Bernhard Korn
BMC Biotechnology 2007, 7:64 (3October2007)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Methodology article
Gene knockdown by RNAi in the pea aphid Acyrthosiphon pisum
Stphanie Jaubert-Possamai, Gal Le Trionnaire, Jol Bonhomme, Georges K Christophides, Claude Rispe, Denis Tagu
BMC Biotechnology 2007, 7:63 (28September2007)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Induction of protective immunity in swine by recombinant bamboo mosaic virus expressing foot-and-mouth disease virus epitopes
Chung-Da Yang, Jia-Teh Liao, Chen-Yen Lai, Ming-Hwa Jong, Chi-Ming Liang, Yeou-Liang Lin, Na-Sheng Lin, Yau-Heiu Hsu, Shu-Mei Liang
BMC Biotechnology 2007, 7:62 (27September2007)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Methodology article
pHUSH: a single vector system for conditional gene expression
Daniel C Gray, Klaus P Hoeflich, Li Peng, Zhenyu Gu, Alvin Gogineni, Lesley J Murray, Mike Eby, Noelyn Kljavin, Somasekar Seshagiri, Mary J Cole, David P Davis
BMC Biotechnology 2007, 7:61 (26September2007)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Methodology article
A hybrid microfluidic-vacuum device for direct interfacing with conventional cell culture methods
Bong Geun Chung, Jeong Won Park, Jia Sheng Hu, Carlos Huang, Edwin S Monuki, Noo Li Jeon
BMC Biotechnology 2007, 7:60 (20September2007)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Methodology article
Scarless and site-directed mutagenesis in Salmonella enteritidis chromosome
Mandy M Cox, Sherryll L Layton, Tieshan Jiang, Kim Cole, Billy M Hargis, Luc R Berghman, Walter G Bottje, Young Min Kwon
BMC Biotechnology 2007, 7:59 (17September2007)
[Abstract] [Full text] [PDF] [PubMed] [Related articles] [Cited on BioMed Central]

Research article
Lactobacillli expressing llama VHH fragments neutralise Lactococcus phages
Anna Hultberg, Denise M Tremblay, Hans de Haard, Theo Verrips, Sylvain Moineau, Lennart Hammarstrm, Harold Marcotte
BMC Biotechnology 2007, 7:58 (17September2007)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Characterization of the effect of sample quality on high density oligonucleotide microarray data using progressively degraded rat liver RNA
Karol L Thompson, P Scott Pine, Barry A Rosenzweig, Yaron Turpaz, Jacques Retief
BMC Biotechnology 2007, 7:57 (13September2007)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Expression of HPV-11 L1 protein in transgenic Arabidopsis thaliana and Nicotiana tabacum
Thomas O Kohl, Inga I Hitzeroth, Neil D Christensen, Edward P Rybicki
BMC Biotechnology 2007, 7:56 (12September2007)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Detection of the pediocin gene pedA in strains from human faeces by real-time PCR and characterization of Pediococcus acidilactici UVA1
Sophie Mathys, Ueli von Ah, Christophe Lacroix, Ern Staub, Raffaella Mini, Tania Cereghetti, Leo Meile
BMC Biotechnology 2007, 7:55 (12September2007)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
New active site oriented glyoxyl-agarose derivatives of Escherichia coli penicillin G acylase
Davide A Cecchini, Immacolata Serra, Daniela Ubiali, Marco Terreni, Alessandra M Albertini
BMC Biotechnology 2007, 7:54 (10September2007)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Comparison of estimation capabilities of response surface methodology (RSM) with artificial neural network (ANN) in lipase-catalyzed synthesis of palm-based wax ester
Mahiran Basri, Raja Noor Zaliha Raja Abd Rahman, Afshin Ebrahimpour, Abu Bakar Salleh, Erin Ryantin Gunawan, Mohd Basyaruddin Abd Rahman
BMC Biotechnology 2007, 7:53 (30August2007)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Methodology article
High-efficiency protein expression in plants from agroinfection-compatible Tobacco mosaic virus expression vectors
John A Lindbo
BMC Biotechnology 2007, 7:52 (27August2007)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Site-directed in vitro immunization leads to a complete human monoclonal IgG4 that binds specifically to the CDR2 region of CTLA-4 (CD152) without interfering the engagement of natural ligands
Li-Te Chin, Chishih Chu, Han-Min Chen, Shu-Ching Hsu, Bor-Chun Weng, Chi-Hong Chu
BMC Biotechnology 2007, 7:51 (23August2007)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
High performance microbiological transformation of L-tyrosine to L-dopa by Yarrowia lipolytica NRRL-143
Sikander Ali, Jeffry L Shultz, Ikram-ul-Haq
BMC Biotechnology 2007, 7:50 (16August2007)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Methodology article
A new enzymatic route for production of long 5'-phosphorylated oligonucleotides using suicide cassettes and rolling circle DNA synthesis
Jakob S Lohmann, Magnus Stougaard, Jrn Koch
BMC Biotechnology 2007, 7:49 (16August2007)
[Abstract] [Full text] [PDF] [PubMed] [Related articles] [Cited on BioMed Central]

Methodology article
One-step selection of Vaccinia virus-binding DNA aptamers by MonoLEX
Andreas Nitsche, Andreas Kurth, Anna Dunkhorst, Oliver Pnke, Hendrik Sielaff, Wolfgang Junge, Doreen Muth, Frieder Scheller, Walter Stcklein, Claudia Dahmen, Georg Pauli, Andreas Kage
BMC Biotechnology 2007, 7:48 (15August2007)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Methodology article
A minimal peach type II chlorophyll a/b-binding protein promoter retains tissue-specificity and light regulation in tomato
Carole L Bassett, Ann M Callahan, Timothy S Artlip, Ralph Scorza, Chinnathambi Srinivasan
BMC Biotechnology 2007, 7:47 (14August2007)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Construction of miniantibodies for the in vivo study of human autoimmune diseases in animal models
Roberto Di Niro, Federica Ziller, Fiorella Florian, Sergio Crovella, Marco Stebel, Marco Bestagno, Oscar Burrone, Andrew RM Bradbury, Paola Secco, Roberto Marzari, Daniele Sblattero
BMC Biotechnology 2007, 7:46 (1August2007)
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Methodology article
Domain selection combined with improved cloning strategy for high throughput expression of higher eukaryotic proteins
Yunjia Chen, Shihong Qiu, Chi-Hao Luan, Ming Luo
BMC Biotechnology 2007, 7:45 (30July2007)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Knockdown of the bovine prion gene PRNP by RNA interference (RNAi) technology
Shizuyo Sutou, Miho Kunishi, Toshiyuki Kudo, Pimprapar Wongsrikeao, Makoto Miyagishi, Takeshige Otoi
BMC Biotechnology 2007, 7:44 (26July2007)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Methodology article
A novel real-time ultrasonic method for prion protein detection using plasminogen as a capture molecule
Carmen Negredo, Eoin Monks, Torres Sweeney
BMC Biotechnology 2007, 7:43 (20July2007)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Enzymatic engineering of the porcine genome with transposons and recombinases
Karl J Clark, Daniel F Carlson, Linda K Foster, Byung-Whi Kong, Douglas N Foster, Scott C Fahrenkrug
BMC Biotechnology 2007, 7:42 (17July2007)
[Abstract] [Full text] [PDF] [PubMed] [Related articles] [Cited on BioMed Central]

Research article
Lentiviral vectors encoding tetracycline-dependent repressors and transactivators for reversible knockdown of gene expression: a comparative study
Krzysztof Pluta, William Diehl, Xian-Yang Zhang, Robert Kutner, Agnieszka Bialkowska, Jakob Reiser
BMC Biotechnology 2007, 7:41 (16July2007)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Methodology article
Laser-scanning velocimetry: A confocal microscopy method for quantitative measurement of cardiovascular performance in zebrafish embryos and larvae
Michael H Malone, Noah Sciaky, Lisa Stalheim, Klaus M Hahn, Elwood Linney, Gary L Johnson
BMC Biotechnology 2007, 7:40 (10July2007)
[Abstract] [Full text] [PDF] [PubMed] [Related articles] [Cited on BioMed Central]

Research article
Population kinetics during simultaneous infection of insect cells with two different recombinant baculoviruses for the production of rotavirus-like particles
Jimmy A Mena, Octavio T Ramrez, Laura A Palomares
BMC Biotechnology 2007, 7:39 (4July2007)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Generation of human scFvs antibodies recognizing a prion protein epitope expressed on the surface of human lymphoblastoid cells
Michela Flego, Alessandro Ascione, Silvia Zamboni, Maria L Dupuis, Valentina Imperiale, Maurizio Cianfriglia
BMC Biotechnology 2007, 7:38 (2July2007)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Single fluorescent protein-based Ca2+ sensors with increased dynamic range
Ekaterina A Souslova, Vsevolod V Belousov, John G Lock, Staffan Strmblad, Sergey Kasparov, Alexey P Bolshakov, Vsevolod G Pinelis, Yulii A Labas, Sergey Lukyanov, Lorenz M Mayr, Dmitriy M Chudakov
BMC Biotechnology 2007, 7:37 (29June2007)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Comparison of miRNA expression patterns using total RNA extracted from matched samples of formalin-fixed paraffin-embedded (FFPE) cells and snap frozen cells
Jinghuan Li, Paul Smyth, Richard Flavin, Susanne Cahill, Karen Denning, Sinead Aherne, Simone M Guenther, John J O'Leary, Orla Sheils
BMC Biotechnology 2007, 7:36 (29June2007)
[Abstract] [Full text] [PDF] [PubMed] [Related articles] [Cited on BioMed Central]

Research article
Cloning and characterization of an adenoviral vector for highly efficient and doxycycline suppressible expression of bioactive human single chain interleukin 12 in colon cancer
Holger Wulff, Thorsten Krieger, Karen Krger, Ingrid Stahmer, Friedrich Thaiss, Hansjrg Schfer, Andreas Block
BMC Biotechnology 2007, 7:35 (26June2007)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Construction and model-based analysis of a promoter library for E. coli: an indispensable tool for metabolic engineering
Marjan De Mey, Jo Maertens, Gaspard J Lequeux, Wim K Soetaert, Erick J Vandamme
BMC Biotechnology 2007, 7:34 (18June2007)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Isolation and characterization of mutated alcohol oxidases from the yeast Hansenula polymorpha with decreased affinity toward substrates and their use as selective elements of an amperometric biosensor
Kostyantyn V Dmytruk, Oleh V Smutok, Olena B Ryabova, Galyna Z Gayda, Volodymyr A Sibirny, Wolfgang Schuhmann, Mykhailo V Gonchar, Andriy A Sibirny
BMC Biotechnology 2007, 7:33 (13June