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	1. 108749 Accesses	Methodology article Statistical analysis of real-time PCR data Joshua S Yuan, Ann Reed, Feng Chen, C Neal Stewart BMC Bioinformatics 2006, 7:85 (22 February 2006) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	2. 105980 Accesses	Methodology Advancing translational research with the Semantic Web Alan Ruttenberg, Tim Clark, William Bug, Matthias Samwald, Olivier Bodenreider, Helen Chen, Donald Doherty, Kerstin Forsberg, Yong Gao, Vipul Kashyap, June Kinoshita, Joanne Luciano, M Scott Marshall, Chimezie Ogbuji, Jonathan Rees, Susie Stephens, Gwendolyn T Wong, Elizabeth Wu, Davide Zaccagnini, Tonya Hongsermeier, Eric Neumann, Ivan Herman, Kei-Hoi Cheung BMC Bioinformatics 2007, 8(Suppl 3):S2 (9 May 2007) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central \| 1 comment \| F1000 Biology
	3. 56406 Accesses	Correspondence Mistaken Identifiers: Gene name errors can be introduced inadvertently when using Excel in bioinformatics Barry R Zeeberg, Joseph Riss, David W Kane, Kimberly J Bussey, Edward Uchio, W Marston Linehan, J Carl Barrett, John N Weinstein BMC Bioinformatics 2004, 5:80 (23 June 2004) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central \| 7 comments
	4. 45954 Accesses	Software MUSCLE: a multiple sequence alignment method with reduced time and space complexity Robert C Edgar BMC Bioinformatics 2004, 5:113 (19 August 2004) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	5. 44486 Accesses	Methodology article Statistical significance of quantitative PCR Yann Karlen, Alan McNair, Sébastien Perseguers, Christian Mazza, Nicolas Mermod BMC Bioinformatics 2007, 8:131 (20 April 2007) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	6. 42357 Accesses	Research article Evaluation of statistical methods for normalization and differential expression in mRNA-Seq experiments James H Bullard, Elizabeth Purdom, Kasper D Hansen, Sandrine Dudoit BMC Bioinformatics 2010, 11:94 (18 February 2010) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	7. 41108 Accesses	Editorial Delayed impact: ISI's citation tracking choices are keeping scientists in the dark Matthew J Cockerill BMC Bioinformatics 2004, 5:93 (12 July 2004) Full text \| PDF \| PubMed \| Cited on BioMed Central
	8. 40860 Accesses	Proceedings An evaluation of human protein-protein interaction data in the public domain Suresh Mathivanan, Balamurugan Periaswamy, TKB Gandhi, Kumaran Kandasamy, Shubha Suresh, Riaz Mohmood, YL Ramachandra, Akhilesh Pandey BMC Bioinformatics 2006, 7(Suppl 5):S19 (18 December 2006) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	9. 40529 Accesses	Software High-throughput sequence alignment using Graphics Processing Units Michael C Schatz, Cole Trapnell, Arthur L Delcher, Amitabh Varshney BMC Bioinformatics 2007, 8:474 (10 December 2007) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	10. 37500 Accesses	Research article The COG database: an updated version includes eukaryotes Roman L Tatusov, Natalie D Fedorova, John D Jackson, Aviva R Jacobs, Boris Kiryutin, Eugene V Koonin, Dmitri M Krylov, Raja Mazumder, Sergei L Mekhedov, Anastasia N Nikolskaya, B Sridhar Rao, Sergei Smirnov, Alexander V Sverdlov, Sona Vasudevan, Yuri I Wolf, Jodie J Yin, Darren A Natale BMC Bioinformatics 2003, 4:41 (11 September 2003) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central \| F1000 Biology
	11. 35698 Accesses	Methodology article A standard curve based method for relative real time PCR data processing Alexey Larionov, Andreas Krause, William Miller BMC Bioinformatics 2005, 6:62 (21 March 2005) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central \| Editor’s summary A standard curve-based method for processing relative real time PCR data is simple and reliable, avoiding the need to measure PCR efficiency and requiring minimal operator input.
	12. 33818 Accesses	Research CUDA compatible GPU cards as efficient hardware accelerators for Smith-Waterman sequence alignment Svetlin A Manavski, Giorgio Valle BMC Bioinformatics 2008, 9(Suppl 2):S10 (26 March 2008) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	13. 33720 Accesses	Research article Metabolic flux balance analysis and the in silico analysis of Escherichia coli K-12 gene deletions Jeremy S Edwards, Bernhard O Palsson BMC Bioinformatics 2000, 1:1-1 (27 July 2000) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	14. 31378 Accesses	Research article The Comparative RNA Web (CRW) Site: an online database of comparative sequence and structure information for ribosomal, intron, and other RNAs Jamie J Cannone, Sankar Subramanian, Murray N Schnare, James R Collett, Lisa M D'Souza, Yushi Du, Brian Feng, Nan Lin, Lakshmi V Madabusi, Kirsten M Müller, Nupur Pande, Zhidi Shang, Nan Yu, Robin R Gutell BMC Bioinformatics 2002, 3:2 (17 January 2002) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	15. 30913 Accesses	Software BioWarehouse: a bioinformatics database warehouse toolkit Thomas J Lee, Yannick Pouliot, Valerie Wagner, Priyanka Gupta, David WJ Stringer-Calvert, Jessica D Tenenbaum, Peter D Karp BMC Bioinformatics 2006, 7:170 (23 March 2006) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	16. 30194 Accesses	Methodology article An automated method for finding molecular complexes in large protein interaction networks Gary D Bader, Christopher WV Hogue BMC Bioinformatics 2003, 4:2 (13 January 2003) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	17. 29505 Accesses	Methodology article Information extraction from full text scientific articles: Where are the keywords? Parantu K Shah, Carolina Perez-Iratxeta, Peer Bork, Miguel A Andrade BMC Bioinformatics 2003, 4:20 (29 May 2003) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	18. 29257 Accesses	Methodology article Noncoding RNA gene detection using comparative sequence analysis Elena Rivas, Sean R Eddy BMC Bioinformatics 2001, 2:8 (10 October 2001) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	19. 28594 Accesses	Research article Hybridization interactions between probesets in short oligo microarrays lead to spurious correlations Michał J Okoniewski, Crispin J Miller BMC Bioinformatics 2006, 7:276 (2 June 2006) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central \| Editor’s summary Mutiple targeting, where multiple probes target a single transcript sequence, is prevalent in microarray studies and must be considered during analysis to avoid false positives resulting from non-independence of measured expression levels.
	20. 27666 Accesses	Methodology article Detecting outliers when fitting data with nonlinear regression – a new method based on robust nonlinear regression and the false discovery rate Harvey J Motulsky, Ronald E Brown BMC Bioinformatics 2006, 7:123 (9 March 2006) Abstract \| Full text \| PDF \| PubMed \| 1 comment
	21. 27362 Accesses	Software WGCNA: an R package for weighted correlation network analysis Peter Langfelder, Steve Horvath BMC Bioinformatics 2008, 9:559 (29 December 2008) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central \| F1000 Biology
	22. 27293 Accesses	Research article Evaluation of genomic island predictors using a comparative genomics approach Morgan GI Langille, William WL Hsiao, Fiona SL Brinkman BMC Bioinformatics 2008, 9:329 (5 August 2008) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	23. 27090 Accesses	Software GEOGLE: context mining tool for the correlation between gene expression and the phenotypic distinction Yao Yu, Kang Tu, Siyuan Zheng, Yun Li, Guohui Ding, Jie Ping, Pei Hao, Yixue Li BMC Bioinformatics 2009, 10:264 (25 August 2009) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	24. 26924 Accesses	Methodology article Gene selection and classification of microarray data using random forest Ramón Díaz-Uriarte, Sara Alvarez de Andrés BMC Bioinformatics 2006, 7:3 (6 January 2006) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	25. 26449 Accesses	Research article Microarray results: how accurate are they? Ravi Kothapalli, Sean J Yoder, Shrikant Mane, Thomas P Loughran BMC Bioinformatics 2002, 3:22 (23 August 2002) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central \| Editor’s summary Analysis of microarray data obtained from two different commercially available systems revealed several inconsistencies suggesting that microarray results should be interpreted cautiously.