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Protein and enzyme biochemistry Section edited by John Honek

Protein and enzyme biochemistry: This section incorporates all aspects of protein and enzyme biochemistry including but not limited to: protein chemistry and proteomics, protein folding and enzyme activity. Applications of these areas, such as in bionanotechnology and biosensors are also welcome.

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1.

Research article   Open Access

Proteomic analysis of differentially expressed proteins in vitamin C-treated AGS cells

Arulkumar Nagappan, Hyeon Park, Kwang Park, Jin A Kim, Gyeong Hong, Sang Kang, Jue Zhang, Eun Kim, Won Lee, Chung Won, Gon Kim BMC Biochemistry 2013, 14:24 (26 September 2013)

Abstract | Full text | PDF | PubMed

2.

Research article   Open Access Highly Accessed

A highly conserved arginine residue of the chitosanase from Streptomyces sp. N174 is involved both in catalysis and substrate binding

Marie-Ève Lacombe-Harvey, Mélanie Fortin, Takayuki Ohnuma, Tamo Fukamizo, Thomas Letzel, Ryszard Brzezinski BMC Biochemistry 2013, 14:23 (16 September 2013)

Abstract | Full text | PDF | PubMed

3.

Research article   Open Access Highly Accessed

Peroxygenase activity of cytochrome c peroxidase and three apolar distal heme pocket mutants: hydroxylation of 1-methoxynaphthalene

James E Erman, Heather Kilheeney, Anil K Bidwai, Caitlan E Ayala, Lidia B Vitello BMC Biochemistry 2013, 14:19 (30 July 2013)

Abstract | Full text | PDF | ePUB | PubMed |  Editor’s summary

Mutating Arg48, Trp51, and His52 in the distal heme pocket of cytochrome C peroxidase to alanines improves the binding affinity of apolar substrates and escalates turnover rate, though the constructs are more susceptible to oxidative degradation.

4.

Research article   Open Access Highly Accessed

Identification of the lamin A/C phosphoepitope recognized by the antibody P-STM in mitotic HeLa S3 cells

Jeng-Ting Chen, Chia-Wen Ho, Lang-Ming Chi, Kun-Yi Chien, Ya-Ju Hsieh, Shih-Jie Lin, Jau-Song Yu BMC Biochemistry 2013, 14:18 (19 July 2013)

Abstract | Full text | PDF | PubMed

5.

Research article   Open Access Highly Accessed

Experimental detection of proteolytic activity in a signal peptide peptidase of Arabidopsis thaliana

Masako Hoshi, Yu Ohki, Keisuke Ito, Taisuke Tomita, Takeshi Iwatsubo, Yoshiro Ishimaru, Keiko Abe, Tomiko Asakura BMC Biochemistry 2013, 14:16 (6 July 2013)

Abstract | Full text | PDF | ePUB | PubMed

6.

Research article   Open Access Highly Accessed

Probing the stability of the “naked” mucin-like domain of human α-dystroglycan

Manuela Bozzi, Enrico Di Stasio, Giovanni Scaglione, Claudia Desiderio, Claudia Martelli, Bruno Giardina, Francesca Sciandra, Andrea Brancaccio BMC Biochemistry 2013, 14:15 (1 July 2013)

Abstract | Full text | PDF | ePUB | PubMed

7.

Research article   Open Access Highly Accessed

Construction of a chimeric thermoacidophilic beta-endoglucanase

Kristina Kufner, Georg Lipps BMC Biochemistry 2013, 14:11 (29 April 2013)

Abstract | Full text | PDF | ePUB | PubMed |  Editor’s summary

Targeted combination of cellulases from Sulfolobus solfataricus and Thermotoga maritima results in a chimera that displays advantageous qualities from both parent enzymes, suggesting that this technique could act as an alternative to point mutational engineering.

8.

Research article   Open Access Highly Accessed

Side chain requirements for affinity and specificity in D5, an HIV-1 antibody derived from the VH1-69 germline segment

Alex Stewart, Joseph S Harrison, Lauren K Regula, Jonathan R Lai BMC Biochemistry 2013, 14:9 (8 April 2013)

Abstract | Full text | PDF | ePUB | PubMed

9.

Research article   Open Access Highly Accessed

Nedd8 processing enzymes in Schizosaccharomyces pombe

Jean E O’Donoghue, Dawadschargal Bech-Otschir, Ida B Larsen, Mairi Wallace, Rasmus Hartmann-Petersen, Colin Gordon BMC Biochemistry 2013, 14:8 (15 March 2013)

Abstract | Full text | PDF | PubMed

10.

Research article   Open Access Highly Accessed

The complete N-terminal extension of heparin cofactor II is required for maximal effectiveness as a thrombin exosite 1 ligand

Amanda J Boyle, Leigh Ann Roddick, Varsha Bhakta, Melissa D Lambourne, Murray S Junop, Patricia C Liaw, Jeffrey I Weitz, William P Sheffield BMC Biochemistry 2013, 14:6 (7 March 2013)

Abstract | Full text | PDF | PubMed |  Editor’s summary

An isolated form of N-terminal acidic extension of Heparin cofactor II (HCII 1-75) binds thrombin with a higher affinity than glycosaminoglycans (GAGs), suggesting that this region of Heparin cofactor II (HCII) is required for binding of thrombin exosite 1.

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