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1.

23029
Accesses

Methodology article   Open Access Highly Accessed

Characterization of a protease produced by a Trichoderma harzianum isolate which controls cocoa plant witches' broom disease

Janice L De Marco, Carlos Felix BMC Biochemistry 2002, 3:3 (22 January 2002)

Abstract | Full text | PDF | PubMed

2.

22032
Accesses

Research article   Open Access Highly Accessed

The molecular basis of electroporation

D Peter Tieleman BMC Biochemistry 2004, 5:10 (19 July 2004)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central | F1000 Biology |  Editor’s summary

A molecular model of electroporation, based on molecular dynamics simulations of phosphoplipid bilayers, demonstrates that strong electric field gradients provide a driving force for water penetration into membranes.

3.

17838
Accesses

Research article   Open Access Highly Accessed

Acetylcholinesterase assay for cerebrospinal fluid using bupivacaine to inhibit butyrylcholinesterase

Wolfram H Kluge, Harald H Kluge, Heike I Bauer, Stefan Pietsch, Jens Anders, Rudolf A Venbrocks BMC Biochemistry 2001, 2:17 (21 December 2001)

Abstract | Full text | PDF | PubMed

4.

16577
Accesses

Research article   Open Access Highly Accessed

The effect of EGTA and Ca++ in regulation of the brain Na/K-ATP-ase by noradrenaline

Shorena Abashidze, Tamar Jariashvili, Zurab Kometiani BMC Biochemistry 2001, 2:8 (3 September 2001)

Abstract | Full text | PDF | PubMed

5.

15836
Accesses

Research article   Open Access Highly Accessed

Measurement of peroxisomal enzyme activities in the liver of brown trout (Salmo trutta), using spectrophotometric methods

Maria Rocha, Eduardo Rocha, Albina D Resende, Alexandre Lobo-da-Cunha BMC Biochemistry 2003, 4:2 (11 March 2003)

Abstract | Full text | PDF | PubMed

6.

14618
Accesses

Methodology article   Open Access Highly Accessed

A simplified method for analysis of polyunsaturated fatty acids

Jing X Kang, Jingdong Wang BMC Biochemistry 2005, 6:5 (24 March 2005)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

7.

14401
Accesses

Research article   Open Access Highly Accessed

Regulation of prostaglandin synthesis and cell adhesion by a tryptophan catabolizing enzyme

Brendan Marshall, Derin Keskin, Andrew L Mellor BMC Biochemistry 2001, 2:5 (17 May 2001)

Abstract | Full text | PDF | PubMed

8.

14174
Accesses

Research article   Open Access Highly Accessed

Color transitions in coral's fluorescent proteins by site-directed mutagenesis

Nadya G Gurskaya, Alexander P Savitsky, Yurii G Yanushevich, Sergey A Lukyanov, Konstantin A Lukyanov BMC Biochemistry 2001, 2:6 (10 July 2001)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central |  Editor’s summary

Sequence comparison, backed up by site-directed mutagenesis, reveals amino acids critical in determining the emission maxima of fluorescent proteins from the coral Anthozoa. This should assist colour prediction for proteins from coral cDNA sequences.

9.

13580
Accesses

Research article   Open Access Highly Accessed

Gene expression profile of HIV-1 Tat expressing cells: a close interplay between proliferative and differentiation signals

Cynthia de la Fuente, Francisco Santiago, Longwen Deng, Carolyne Eadie, Irene Zilberman, Kylene Kehn, Anil Maddukuri, Shanese Baylor, Kaili Wu, Chee Lee, Anne Pumfery, Fatah Kashanchi BMC Biochemistry 2002, 3:14 (10 June 2002)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

10.

12955
Accesses

Methodology article   Open Access Highly Accessed

Specificity of DNA triple helix formation analyzed by a FRET assay

Sabine Reither, Albert Jeltsch BMC Biochemistry 2002, 3:27 (12 September 2002)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

11.

11980
Accesses

Research article   Open Access Highly Accessed

The PEST sequence does not contribute to the stability of the cystic fibrosis transmembrane conductance regulator

Eva Y Chen, David M Clarke BMC Biochemistry 2002, 3:29 (2 October 2002)

Abstract | Full text | PDF | PubMed

12.

11492
Accesses

Research article   Open Access Highly Accessed

Characterization of a β-glucanase produced by Rhizopus microsporus var. microsporus, and its potential for application in the brewing industry

Klecius Celestino, Ricardo B Cunha, Carlos R Felix BMC Biochemistry 2006, 7:23 (5 December 2006)

Abstract | Full text | PDF | PubMed

13.

11435
Accesses

Research article   Open Access Highly Accessed

Characterization of the aggregates formed during recombinant protein expression in bacteria

Andrea Schrödel, Ario de Marco BMC Biochemistry 2005, 6:10 (31 May 2005)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

14.

11380
Accesses

Research article   Open Access Highly Accessed

Src Kinase becomes preferentially associated with the VEGFR, KDR/Flk-1, following VEGF stimulation of vascular endothelial cells

Mary T Chou, Jing Wang, Donald J Fujita BMC Biochemistry 2002, 3:32 (31 December 2002)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

15.

11118
Accesses

Research article   Open Access Highly Accessed

Function, expression and localization of annexin A7 in platelets and red blood cells: Insights derived from an annexin A7 mutant mouse

Claudia Herr, Christoph S Clemen, Gisela Lehnert, Rüdiger Kutschkow, Susanne M Picker, Birgit S Gathof, Carlotta Zamparelli, Michael Schleicher, Angelika A Noegel BMC Biochemistry 2003, 4:8 (19 August 2003)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

16.

11044
Accesses

Research article   Open Access Highly Accessed

The fission yeast COP9/signalosome is involved in cullin modification by ubiquitin-related Ned8p

Chunshui Zhou, Volker Seibert, Rory Geyer, Edward Rhee, Svetlana Lyapina, Greg Cope, Raymond J Deshaies, Dieter A Wolf BMC Biochemistry 2001, 2:7 (18 July 2001)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central |  Editor’s summary

Multiprotein complex COP9/signalosome is a global regulator in the control of cullin modification by Ned8p. This complex probably regulates the activity of multiple cullin ubiquitin ligases through counteracting their stimulatory Ned8p modification.

17.

10842
Accesses

Research article   Open Access Highly Accessed

Plasma lipases and lipid transfer proteins increase phospholipid but not free cholesterol transfer from lipid emulsion to high density lipoproteins.

Valéria S Nunes, Eder CR Quintão, Patrícia M Cazita, Lila M Harada, Eliana C de Faria, Helena CF Oliveira BMC Biochemistry 2001, 2:1 (20 February 2001)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

18.

10635
Accesses

Methodology article   Open Access Highly Accessed

A direct method to visualise the aryl acylamidase activity on cholinesterases in polyacrylamide gels

Lakshmanan Jaganathan, Rathanam Boopathy BMC Biochemistry 2000, 1:3 (20 December 2000)

Abstract | Full text | PDF | PubMed

19.

10520
Accesses

Research article   Open Access

The regulation of protein synthesis and translation factors by CD3 and CD28 in human primary T lymphocytes

Miranda Kleijn, Christopher G Proud BMC Biochemistry 2002, 3:11 (17 May 2002)

Abstract | Full text | PDF | PubMed

20.

10870
Accesses

Research article   Open Access

DNA loops and semicatenated DNA junctions

Claire Gaillard, François Strauss BMC Biochemistry 2000, 1:1-1 (19 July 2000)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

21.

10181
Accesses

Research article   Open Access

Suppression of nitric oxide production in mouse macrophages by soybean flavonoids accumulated in response to nitroprusside and fungal elicitation

Loren S Scuro, PU Simioni, DL Grabriel, Elzira E Saviani, Luzia V Modolo, Wirla MSC Tamashiro, Ione Salgado BMC Biochemistry 2004, 5:5 (21 April 2004)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

22.

9698
Accesses

Research article   Open Access Highly Accessed

Analysis of phosphorylation of human heat shock factor 1 in cells experiencing a stress

Toumy Guettouche, Frank Boellmann, William S Lane, Richard Voellmy BMC Biochemistry 2005, 6:4 (11 March 2005)

Abstract | Full text | PDF | PubMed | 1 comment |  Editor’s summary

Alanine scanning and mass spectrometry analysis identify phosphorylation of twelve serine residues in human heat shock factor 1 upon heat stress and further functional studies suggest serine-326 plays a critical part in activation of the factor's transcriptional competence

23.

9658
Accesses

Research article   Open Access Highly Accessed

Mitochondrial activities in human cultured skin fibroblasts contaminated by Mycoplasma hyorhinis

Niklas Darin, Norman Kadhom, Jean-Jacques Brière, Dominique Chretien, Cécile M Bébéar, Agnès Rötig, Arnold Munnich, Pierre Rustin BMC Biochemistry 2003, 4:15 (4 November 2003)

Abstract | Full text | PDF | PubMed

24.

9430
Accesses

Review   Open Access Highly Accessed

Patented small molecule inhibitors in the ubiquitin proteasome system

Philippe Guédat, Frédéric Colland BMC Biochemistry 2007, 8(Suppl 1):S14 (22 November 2007)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

25.

9365
Accesses

Research article   Open Access Highly Accessed

Physicochemical characterization of the endotoxins from Coxiella burnetii strain Priscilla in relation to their bioactivities

Rudolf Toman, Patrick Garidel, Jörg Andrä, Katarina Slaba, Ahmed Hussein, Michel HJ Koch, Klaus Brandenburg BMC Biochemistry 2004, 5:1 (12 January 2004)

Abstract | Full text | PDF | PubMed

26.

9342
Accesses

Research article   Open Access Highly Accessed

The DNA polymerase activity of Pol ε holoenzyme is required for rapid and efficient chromosomal DNA replication in Xenopus egg extracts

Koh Shikata, Taro Sasa-Masuda, Yukiko Okuno, Shou Waga, Akio Sugino BMC Biochemistry 2006, 7:21 (22 August 2006)

Abstract | Full text | PDF | PubMed

27.

9327
Accesses

Research article   Open Access Highly Accessed

Mutation of exposed hydrophobic amino acids to arginine to increase protein stability

Caroline Strub, Carole Alies, Andrée Lougarre, Caroline Ladurantie, Jerzy Czaplicki, Didier Fournier BMC Biochemistry 2004, 5:9 (13 July 2004)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

28.

9255
Accesses

Research article   Open Access Highly Accessed

Low solubility of unconjugated bilirubin in dimethylsulfoxide – water systems: implications for pKa determinations

Pasupati Mukerjee, J Donald Ostrow, Claudio Tiribelli BMC Biochemistry 2002, 3:17 (17 June 2002)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

29.

9240
Accesses

Research article   Open Access

Isolation and characterization of a novel lepidopteran-selective toxin from the venom of South Indian red scorpion, Mesobuthus tamulus

Rajendra Wudayagiri, Bora Inceoglu, Rafael Herrmann, Maher Derbel, Prabhakara V Choudary, Bruce D Hammock BMC Biochemistry 2001, 2:16 (17 December 2001)

Abstract | Full text | PDF | PubMed

30.

9157
Accesses

Review   Open Access Highly Accessed

The ubiquitin system, disease, and drug discovery

Matthew D Petroski BMC Biochemistry 2008, 9(Suppl 1):S7 (21 October 2008)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

31.

8847
Accesses

Research article   Open Access Highly Accessed

The effects of NaCl concentration and pH on the stability of hyperthermophilic protein Ssh10b

Yong-Jin Mao, Xiang-Rong Sheng, Xian-Ming Pan BMC Biochemistry 2007, 8:28 (21 December 2007)

Abstract | Full text | PDF | PubMed

32.

8801
Accesses

Research article   Open Access Highly Accessed

Glycosaminoglycans in human retinoblastoma cells: Heparan sulfate, a modulator of the pigment epithelium-derived factor-receptor interactions

Elena M Alberdi, John E Weldon, S Patricia Becerra BMC Biochemistry 2003, 4:1 (19 February 2003)

Abstract | Full text | PDF | PubMed

33.

8567
Accesses

Research article   Open Access

Probing substrate binding to Metallo-β-Lactamase L1 from Stenotrophomonas maltophilia by using site-directed mutagenesis

Anne L Carenbauer, James D Garrity, Gopal Periyannan, Robert B Yates, Michael W Crowder BMC Biochemistry 2002, 3:4 (13 February 2002)

Abstract | Full text | PDF | PubMed

34.

8466
Accesses

Research article   Open Access Highly Accessed

Metabolic signature of breast cancer cell line MCF-7: profiling of modified nucleosides via LC-IT MS coupling

Dino Bullinger, Hans Neubauer, Tanja Fehm, Stefan Laufer, Christoph H Gleiter, Bernd Kammerer BMC Biochemistry 2007, 8:25 (29 November 2007)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

35.

8427
Accesses

Review   Open Access Highly Accessed

HPV E6, E6AP and cervical cancer

Sylvie Beaudenon, Jon M Huibregtse BMC Biochemistry 2008, 9(Suppl 1):S4 (21 October 2008)

Abstract | Full text | PDF | PubMed

36.

8423
Accesses

Research article   Open Access Highly Accessed

The FTO (fat mass and obesity associated) gene codes for a novel member of the non-heme dioxygenase superfamily

Luis Sanchez-Pulido, Miguel A Andrade-Navarro BMC Biochemistry 2007, 8:23 (8 November 2007)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

37.

8412
Accesses

Research article   Open Access Highly Accessed

Aquaporin-11: A channel protein lacking apparent transport function expressed in brain

Daniel A Gorelick, Jeppe Praetorius, Takashi Tsunenari, Søren Nielsen, Peter Agre BMC Biochemistry 2006, 7:14 (1 May 2006)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central |  Editor’s summary

Although mammalian aquaporin-11 is related to integral membrane transport proteins, it lacks a transport function when expressed in Xenopus oocyte plasma membranes and localizes intracellularly within neurons, so it may represent a functionally distinct subfamily.

38.

8362
Accesses

Research article   Open Access Highly Accessed

Interactions between co-expressed Arabidopsis sucrose transporters in the split-ubiquitin system

Waltraud X Schulze, Anke Reinders, John Ward, Sylvie Lalonde, Wolf B Frommer BMC Biochemistry 2003, 4:3 (18 March 2003)

Abstract | Full text | PDF | PubMed |  Editor’s summary

The split-ubiquitin system provides evidence of hetero- and homo-oligomerization among the three sucrose transporters of Arabidopsis expressed in yeast.

39.

8309
Accesses

Methodology article   Open Access

Methodological factors influencing measurement and processing of plasma reelin in humans

Giovanni Lugli, Jacqueline M Krueger, John M Davis, Antonio M Persico, Flavio Keller, Neil R Smalheiser BMC Biochemistry 2003, 4:9 (7 September 2003)

Abstract | Full text | PDF | PubMed

40.

8293
Accesses

Research article   Open Access

β1 integrins show specific association with CD98 protein in low density membranes

Tatiana V Kolesnikova, Brian A Mannion, Fedor Berditchevski, Martin E Hemler BMC Biochemistry 2001, 2:10 (15 October 2001)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central |  Editor’s summary

Cell surface proteins CD98 selectively associate with ß1 integrins, in the context of low density membranes. This could explain the effect of CD98 on the modulation of integrin binding, signalling and possibly T cell co-stimulation.

41.

8232
Accesses

Research article   Open Access Highly Accessed

A novel form of the membrane protein CD147 that contains an extra Ig-like domain and interacts homophilically

S Hanna, Peter Kirk, Oliver J Holt, Michael J Puklavec, Marion H Brown, A Barclay BMC Biochemistry 2003, 4:17 (7 November 2003)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

42.

8225
Accesses

Research article   Open Access

Binding specificity of Bacillus thuringiensis Cry1Aa for purified, native Bombyx mori aminopeptidase N and cadherin-like receptors

Jeremy L Jenkins, Donald H Dean BMC Biochemistry 2001, 2:12 (16 October 2001)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

43.

8166
Accesses

Research article   Open Access Highly Accessed

Osmotic stress-dependent serine phosphorylation of the histidine kinase homologue DokA

Felix Oehme, Stephan C Schuster BMC Biochemistry 2001, 2:2 (16 March 2001)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

44.

8107
Accesses

Research article   Open Access Highly Accessed

Inhibition of the MEK1/ERK pathway reduces arachidonic acid release independently of cPLA2 phosphorylation and translocation

John H Evans, Daniel J Fergus, Christina C Leslie BMC Biochemistry 2002, 3:30 (8 October 2002)

Abstract | Full text | PDF | PubMed

45.

8061
Accesses

Research article   Open Access

Hibernation impact on the catalytic activities of the mitochondrial D-3-hydroxybutyrate dehydrogenase in liver and brain tissues of jerboa (Jaculus orientalis)

Mostafa Kabine, M'hammed El Kebbaj, Assia Hafiani, Norbert Latruffe, Mustapha Cherkaoui-Malki BMC Biochemistry 2003, 4:11 (10 September 2003)

Abstract | Full text | PDF | PubMed

46.

8049
Accesses

Methodology article   Open Access Highly Accessed

RNA integrity as a quality indicator during the first steps of RNP purifications : A comparison of yeast lysis methods

Miguel López de Heredia, Ralf-Peter Jansen BMC Biochemistry 2004, 5:14 (1 October 2004)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

47.

7987
Accesses

Methodology article   Open Access Highly Accessed

Farnesylation or geranylgeranylation? Efficient assays for testing protein prenylation in vitro and in vivo

Wolfgang Benetka, Manfred Koranda, Sebastian Maurer-Stroh, Fritz Pittner, Frank Eisenhaber BMC Biochemistry 2006, 7:6 (28 February 2006)

Abstract | Full text | PDF | PubMed

48.

7985
Accesses

Research article   Open Access Highly Accessed

Sugar recognition by human galactokinase

David J Timson, Richard J Reece BMC Biochemistry 2003, 4:16 (4 November 2003)

Abstract | Full text | PDF | PubMed

49.

7888
Accesses

Research article   Open Access Highly Accessed

The pyrroloquinoline quinone biosynthesis pathway revisited: A structural approach

Sandra Puehringer, Moritz Metlitzky, Robert Schwarzenbacher BMC Biochemistry 2008, 9:8 (27 March 2008)

Abstract | Full text | PDF | PubMed |  Editor’s summary

Putative roles for six proteins in the biosynthesis pathway of pyrroloquinoline quinone, a bacterial redox-active cofactor for numerous alcohol and aldose dehydrogenases, have been assigned in Klebsiella by integrating structural, genomic and functional data.

50.

7819
Accesses

Research article   Open Access Highly Accessed

Preferential inhibition of xanthine oxidase by 2-amino-6-hydroxy-8-mercaptopurine and 2-amino-6-purine thiol

Sukirti Kalra, Gopabandhu Jena, Kulbhushan Tikoo, Anup Mukhopadhyay BMC Biochemistry 2007, 8:8 (18 May 2007)

Abstract | Full text | PDF | PubMed

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