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Archazolid and apicularen: Novel specific V-ATPase inhibitors
Markus Huss, Florenz Sasse, Brigitte Kunze, Rolf Jansen, Heinrich Steinmetz, Gudrun Ingenhorst, Axel Zeeck, Helmut Wieczorek BMC Biochemistry 2005, 6:13 (4 August 2005)
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Editor’s summary
Archazolid A and B as well as apicularens A and B, two classes of antibiotic macrolactones derived from different myxobacterial species, specifically inhibit V-ATPase, having no affect on the activity of mitochondrial F-ATPase or Na+/K+-ATPase.
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Characterization of 17α-hydroxysteroid dehydrogenase activity (17α-HSD) and its involvement in the biosynthesis of epitestosterone
Véronique Bellemare, Frédérick Faucher, Rock Breton, Van Luu-The BMC Biochemistry 2005, 6:12 (14 July 2005)
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Determination of thermodynamic parameters of Xerocomus chrysenteron lectin interactions with N-acetylgalactosamine and Thomsen-Friedenreich antigen by isothermal titration calorimetry
Luminita Damian, Didier Fournier, Mathias Winterhalter, Laurent Paquereau BMC Biochemistry 2005, 6:11 (1 June 2005)
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Characterization of the aggregates formed during recombinant protein expression in bacteria
Andrea Schrödel, Ario de Marco BMC Biochemistry 2005, 6:10 (31 May 2005)
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Pattern similarity study of functional sites in protein sequences: lysozymes and cystatins
Shuryo Nakai, Eunice CY Li-Chan, Jinglie Dou BMC Biochemistry 2005, 6:9 (18 May 2005)
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Rational polynomial representation of ribonucleotide reductase activity
Tomas Radivoyevitch, Ossama B Kashlan, Barry S Cooperman BMC Biochemistry 2005, 6:8 (6 May 2005)
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Stimulation of Myc transactivation by the TATA binding protein in promoter-reporter assays
John F Barrett, Linda A Lee, Chi V Dang BMC Biochemistry 2005, 6:7 (5 May 2005)
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Editor’s summary
Although transcriptional transactivation by the c-Myc oncoprotein in reporter assays is generally weak, addition of the TATA-binding protein TBP strongly stimulates this activity and might therefore help in the hunt for Myc target genes.
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The de novo methylation activity of Dnmt3a is distinctly different than that of Dnmt1
Chih-Lin Hsieh BMC Biochemistry 2005, 6:6 (30 March 2005)
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A simplified method for analysis of polyunsaturated fatty acids
Jing X Kang, Jingdong Wang BMC Biochemistry 2005, 6:5 (24 March 2005)
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Analysis of phosphorylation of human heat shock factor 1 in cells experiencing a stress
Toumy Guettouche, Frank Boellmann, William S Lane, Richard Voellmy BMC Biochemistry 2005, 6:4 (11 March 2005)
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Editor’s summary
Alanine scanning and mass spectrometry analysis identify phosphorylation of twelve serine residues in human heat shock factor 1 upon heat stress and further functional studies suggest serine-326 plays a critical part in activation of the factor's transcriptional competence
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Correction: Lipid phosphate phosphatases dimerise, but this interaction is not required for in vivo activity
C Burnett, P Makridou, L Hewlett, K Howard BMC Biochemistry 2005, 6:3 (3 March 2005)
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Analysis of leukocyte membrane protein interactions using protein microarrays
Michelle Letarte, Despina Voulgaraki, Deborah Hatherley, Mildred Foster-Cuevas, Nigel J Saunders, A Neil Barclay BMC Biochemistry 2005, 6:2 (1 March 2005)
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Increasing stability of water-soluble PQQ glucose dehydrogenase by increasing hydrophobic interaction at dimeric interface
Shunsuke Tanaka, Satoshi Igarashi, Stefano Ferri, Koji Sode BMC Biochemistry 2005, 6:1 (16 February 2005)
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Analysis of the kinetic mechanism of recombinant human isoprenylcysteine carboxylmethyltransferase (Icmt)
Rudi A Baron, Patrick J Casey BMC Biochemistry 2004, 5:19 (29 December 2004)
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Identification of α-type subunits of the Xenopus 20S proteasome and analysis of their changes during the meiotic cell cycle
Yuka Wakata, Mika Tokumoto, Ryo Horiguchi, Katsutoshi Ishikawa, Yoshitaka Nagahama, Toshinobu Tokumoto BMC Biochemistry 2004, 5:18 (17 December 2004)
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Rhodobacter capsulatus porphobilinogen synthase, a high activity metal ion independent hexamer
David W Bollivar, Cheryl Clauson, Rachel Lighthall, Siiri Forbes, Bashkim Kokona, Robert Fairman, Lenka Kundrat, Eileen K Jaffe BMC Biochemistry 2004, 5:17 (22 November 2004)
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Editor’s summary
Porphobilinogen synthase from Rhodobacter capsulatus is unusual in not requiring a metal ion for activity and in forming hexamers rather than the octamers formed by its counterparts in other organisms.
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The "Transport Specificity Ratio": a structure-function tool to search the protein fold for loci that control transition state stability in membrane transport catalysis
Steven C King BMC Biochemistry 2004, 5:16 (17 November 2004)
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Editor’s summary
The "Transport Specificity Ratio" assay can find positions in proteins that control transition state stability in the catalysis of membrane transport.
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Selective inhibition of c-Myb DNA-binding by RNA polymers
Oddmund Nordgård, Tor Ø Andersen, Odd S Gabrielsen BMC Biochemistry 2004, 5:15 (4 November 2004)
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RNA integrity as a quality indicator during the first steps of RNP purifications : A comparison of yeast lysis methods
Miguel López de Heredia, Ralf-Peter Jansen BMC Biochemistry 2004, 5:14 (1 October 2004)
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Site-specific mutagenesis of Drosophila proliferating cell nuclear antigen enhances its effects on calf thymus DNA polymerase δ
Dmitry Mozzherin, Maeve McConnell, Holly Miller, Paul A Fisher BMC Biochemistry 2004, 5:13 (13 August 2004)
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A single amino acid determines preference between phospholipids and reveals length restriction for activation ofthe S1P4 receptor
Gill Holdsworth, Daniel A Osborne, TrucChi Pham, James I Fells, Gillian Hutchinson, Graeme Milligan, Abby L Parrill BMC Biochemistry 2004, 5:12 (6 August 2004)
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Characterization of yeast histone H3-specific type B histone acetyltransferases identifies an ADA2-independent Gcn5p activity
Amy R Sklenar, Mark R Parthun BMC Biochemistry 2004, 5:11 (26 July 2004)
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The molecular basis of electroporation
D Peter Tieleman BMC Biochemistry 2004, 5:10 (19 July 2004)
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| F1000 Biology
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Editor’s summary
A molecular model of electroporation, based on molecular dynamics simulations of phosphoplipid bilayers, demonstrates that strong electric field gradients provide a driving force for water penetration into membranes.
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Mutation of exposed hydrophobic amino acids to arginine to increase protein stability
Caroline Strub, Carole Alies, Andrée Lougarre, Caroline Ladurantie, Jerzy Czaplicki, Didier Fournier BMC Biochemistry 2004, 5:9 (13 July 2004)
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The VSFASSQQ motif confers calcium sensitivity to the intracellular apyrase LALP70
Annette Biederbick, Ralf Rösser, Jörg Storre, Hans-Peter Elsässer BMC Biochemistry 2004, 5:8 (16 June 2004)
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