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Oral presentation

The osmosensitive phenotype of the hog1 strain is suppressed at elevated temperature [1]. We have shown that the same holds true for the other commonly used HOG pathway mutant strains pbs2 and sho1ssk2ssk22 but not for ste11ssk2ssk22. Intriguingly, the ste11ssk2ssk2 strain displayed a hyper-osmosensitive phenotype at 37°C. This phenotype is suppressed by overexpression of LRE1,HLR1 and WSC3 all genes known to influence cell wall composition. The suppression of the temperature-induced hyper-osmosensitivity by these genes prompted to investigate the role of STE11 and other HOG pathway components in cellular integrity and indeed we have shown that HOG pathway mutants display sensitivity to cell wall-degrading enzymes. LRE1, HLR1 were also shown to suppress the cell wall phenotypes associated with the HOG pathway mutants. The isolated multicopy suppressor genes also suppress temperature-induced cell lysis phenotypes of PKC pathway mutants which could be an indication for shared targets of the PKC pathway and high osmolarity response routes.

References

1. Siderius M, Van Wuytswinkel O, Reijenga KA, Kelders M, Mager WH: The control of intracellular glycerol in Saccharomyces cerevisiae influences osmotic stress response and resistance to increased temperature.

   Molecular Microbiology 2000, 36:1381-1390 .

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