Sensor proteins that detect cGMP are valuable tools to analyse the molecular mechanisms underlying cGMP’s manifold physiological functions. These cGMP sensors allow for visualization and quantification of cGMP in living cells, in real time, and at high spatial and temporal resolution. For instance, they allow one to study sub-cellular cGMP compartments, and combined with multi-photon microscopy, they can provide new insights into complex biological processes, which can be analysed only in live animals.
To monitor cGMP signals in vivo, we have generated and characterized transgenic mouse lines expressing the fluorescence resonance energy transfer (FRET)-based cGi500 sensor (
Primary cells isolated from cGi500-expressing mice, including platelets, neural cells, and smooth muscle cells were used for FRET imaging experiments. NO-induced cGMP synthesis via soluble guanylyl cyclase was observed in the majority of cell types, while cGMP responses to atrial or C-type natriuretic peptides differed between cell types. Moreover, NO-induced cGMP elevations could be observed in the vasculature of retinas isolated from cGi500-expressing animals. Importantly, the feasibility of FRET-based cGMP imaging in living animals was demonstrated by intravital microscopy of anesthetized cGi500-transgenic mice. By epifluorescence FRET microscopy we could visualize transient NO-induced cGMP signals in arterial walls of the cremaster muscle. Furthermore, we used multi-photon microscopy in a dorsal skinfold chamber model and were able to record NO-elicited FRET changes in subcutaneous vessels.
All in all, these cGMP sensor mouse lines represent valuable resources for studies with cGi500-expressing primary cells, and, importantly, they can also be used for intravital imaging studies with live animals. We believe that these mouse lines will find widespread applications and that in combination with other genetic mouse models they can contribute to improve our understanding of cGMP-modulated physiological or disease-associated processes.