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Open Access Research article

Anvirzel™ in combination with cisplatin in breast, colon, lung, prostate, melanoma and pancreatic cancer cell lines

Panagiotis Apostolou1, Maria Toloudi1, Marina Chatziioannou1, Eleni Ioannou1, Dennis R Knocke2, Joe Nester2, Dimitrios Komiotis3 and Ioannis Papasotiriou1*

Author Affiliations

1 Research Genetic Cancer Centre Ltd (R.G.C.C. Ltd), Filotas, Florina, Greece

2 Nerium Biotechnology, Inc. San Antonio, Texas, USA

3 Department of Biochemistry & Biotechnology, University of Thessaly, Larisa, Greece

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BMC Pharmacology and Toxicology 2013, 14:18  doi:10.1186/2050-6511-14-18

Published: 25 March 2013

Abstract

Background

Platinum derivatives are used widely for the treatment of many cancers. However, the toxicity that is observed makes imperative the need for new drugs, or new combinations. Anvirzel™ is an extract which has been demonstrated with experimental data that displays anticancer activity. The aim of the present study is to determine whether the combination of Cisplatin and Anvirzel™ has a synergistic effect against different types of cancer.

Materials and methods

To measure the efficacy of treatment with Cisplatin and Anvirzel™, methyl-tetrazolium dye (MTT) chemosensitivity assays were used incorporating established human cancer cell lines. Measurements were performed in triplicates, three times, using different incubation times and different concentrations of the two formulations in combination or on their own. t-test was used for statistical analysis.

Results

In the majority of the cell lines tested, lower concentrations of Anvirzel™ induced a synergistic effect when combined with low concentrations of Cisplatin after an incubation period of 48 to 72 h. The combination of Anvirzel™/Cisplatin showed anti-proliferative effects against a wide range of tumours.

Conclusion

The results showed that the combination of Anvirzel™ and Cisplatin is more effective than monotherapy, even when administered at low concentrations; thus, undesirable toxic effects can be avoided.

Keywords:
Anvirzel™; Cisplatin; Viability assays; Cancer cell lines; Methyl-tetrazolium dye