Table 1

Assay protocols and DMR signals used for similarity analysis
Cell Probe, pretreatment duration DMR readout Labels used in clustering Figures
HEK293 0.1% DMSO in buffer, 1 hr Ligand, 10 μM HEK-3, 9, 30 Additional file 1: Figure S1, S2
Opioid* 0.1% DMSO in buffer, 1hr Ligand, 10 μM Buffer-3, 9, 30 Figures 1567
HEK-MOR 10 μM ligand, 1 hr DAMGO, 10 μM MOR-3, 9, 30 Additional file 1: Figures S2 & S3
HEK-DOR 10 μM ligand, 1 hr DPDPE, 10 μM DOR-3, 9, 30 Figure 5
HEK-KOR 10 μM ligand, 1 hr BRL-57532, 10 μM KOR-3, 9, 30 Figure 6
SH-SY5Y 10 μM ligand, 1 hr DAMGO, 10 μM 5Y-3, 9, 30 Figure 7
Opioid* 100 ng/ml PTX, 20 hr Ligand, 10 μM PTX-3, 9, 30 Figures  56 and 7
Opioid* 400 ng/ml CTX, 20 hr Ligand, 10 μM CTX-3, 9, 30 Figures 56 and 7
Opioid* 10 μM forskolin, 1 hr Ligand, 10 μM FSK-3, 9, 30 Figures 56 and 7
Opioid* 10 μM U0126, 1 hr Ligand, 10 μM U0126-3, 9, 30 Figures 56 and 7
Opioid* 10 μM SB202190, 1 hr Ligand, 10 μM SB-3, 9, 30 Figures 56 and 7
Opioid* 10 μM SP600125, 1 hr Ligand, 10 μM SP-3, 9, 30 Figures 56 and 7
Opioid* 10 μM LY294002, 1 hr Ligand, 10 μM LY-3, 9, 30 Figures 56 and 7

* Opioid receptor expressing cell lines wherein the same assay protocol was applied.

Morse et al.

Morse et al. BMC Pharmacology and Toxicology 2013 14:17   doi:10.1186/2050-6511-14-17

Open Data