Workflow of 3D quantitative DNA methylation imaging and analysis. Image data acquired by high-resolution microscopy is subjected to a pre-processing step, in which cell nuclei (as areas of interest) are segmented, followed by DNA methylation phenotyping. This step comprises three modules, by which recorded signals in the MeC and DAPI channels are extracted for measuring: global MeC load, MeC/DAPI signal codistribution, and MeC and DAPI signal topology within the nuclear space. The retrieved information is used to assess the capacity of a drug for DNA demethylation and concurrent chromatin reorganization.
Gertych et al. BMC Pharmacology and Toxicology 2013 14:11 doi:10.1186/2050-6511-14-11