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This article is part of the supplement: 18th Scientific Symposium of the Austrian Pharmacological Society (APHAR)

Open Access Meeting abstract

Peptidomics screening for the discovery of uterotonic plant peptides

Johannes Koehbach1, Alfred F Attah12, Margaret O’Brien3, Michael Freissmuth1 and Christian W Gruber1*

Author Affiliations

1 Center for Physiology and Pharmacology, Medical University of Vienna, 1090 Vienna, Austria

2 Department of Pharmacognosy, Faculty of Pharmacy, University of Ibadan, Ibadan, Oyo State, Nigeria

3 National University of Ireland, National Center for Biomedical and Engineering Science, Galway, Ireland

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BMC Pharmacology and Toxicology 2012, 13(Suppl 1):A89  doi:10.1186/2050-6511-13-S1-A89

The electronic version of this article is the complete one and can be found online at: http://www.biomedcentral.com/2050-6511/13/S1/A89


Published:17 September 2012

© 2012 Koehbach et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Background

Drug discovery from natural products is still one of the biggest sources of novel lead compounds. In particular, plant cyclotides, disulfide-rich peptides comprising three conserved disulfide bonds in a knotted arrangement, known as cyclic cystine knot motif, and a head-to-tail cyclization, have been extensively investigated over the last four decades for their use as scaffolds in drug development. However, their distribution among flowering plants still remains limited to few species of the families of Rubiaceae (coffee), Violaceae (violet), Cucurbitaceae (cucurbit), Fabaceae (bean) and recently Solanaceae (potato family), but it is very likely that cyclotides are more widely distributed since their predicted number in Rubiaceae alone is ~50.000. Additionally, the pharmacological validation of plants used in traditional medicines may trigger the discovery of novel uterotonic compounds [1].

Methods and results

Based on the use of plants in traditional Nigerian medicine during pregnancy and childbirth, we analyzed several plants from different families to evaluate their uterotonic properties at cellular level and to identify cyclotides as active molecules. Using a MALDI-TOF/TOF-based screening protocol we were able to identify many novel cyclotide-containing species which was confirmed by manual de novo sequencing and automated database identification. The aqueous extracts and semipure peptide fractions have been tested further in a collagen gel contractility assay model and showed varying ability to induce contractions in human myometrial smooth muscle cells.

Conclusions

In conclusion, our results underpin earlier suggestions that cyclotides are one of the largest peptide classes within plants, covering a large chemical space based on their high sequence diversity. The evaluation of contractile properties of plants used in traditional medicines offers new starting points for the discovery and development of peptide-based uterotonic drug leads.

Acknowledgements

This work is funded by the Austrian Science Fund (FWF, grant P22889) and an “Ernst-Mach” Scholarship from the Austrian Agency for International Mobility and Cooperation in Education, Science and Research (OeAD) (A.A.).

References

  1. Gruber CW, O'Brien M: Uterotonic plants and their bioactive constituents.

    Planta Med 2011, 77:207-220. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL