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This article is part of the supplement: 18th Scientific Symposium of the Austrian Pharmacological Society (APHAR)

Open Access Meeting abstract

Expression of organic cation transporter 3 (SLC22A3) and plasma membrane monoamine transporter (SLC29A4) in human umbilical vein endothelial cells and their relevance for histamine uptake

Polonca Ferk1, Metoda Lipnik-Štangelj2, Mojca Kržan2 and Katarina Černe2*

Author Affiliations

1 Department of Pharmacology and Experimental Toxicology, Faculty of Medicine, University of Maribor, 2000 Maribor, Slovenia

2 Institute of Pharmacology and Experimental Toxicology, Faculty of Medicine, University of Ljubljana, 1000 Ljubljana, Slovenia

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BMC Pharmacology and Toxicology 2012, 13(Suppl 1):A73  doi:10.1186/2050-6511-13-S1-A73

The electronic version of this article is the complete one and can be found online at: http://www.biomedcentral.com/2050-6511/13/S1/A73


Published:17 September 2012

© 2012 Ferk et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Background

Increased plasma histamine levels lead to pathological events. Endothelial cells actively participate in histamine clearance by promoting its uptake via yet unidentified carriers, thus limiting histamine effects. The organic cation transporter 3 (OCT3) and plasma membrane monoamine transporter (PMAT) are the two most prominent transporters for endogenous monoamines. OCT3 and PMAT show Na+/K+-ATPase independency. Both are highly sensitive to inhibition by the isocyanine compound, decynium-22. However, OCT3 is highly sensitive to corticosteron, whereas PMAT is not. We showed in the past that decynium-22 inhibits histamine uptake in cultured human umbilical vascular endothelial cells (HUVEC). In the present study we identified the expression of OCT3 and PMAT in freshly isolated and cultured HUVEC as well as some characteristics of histamine uptake in cultured HUVEC such as ouabain and corticosteron sensitivity.

Methods

We used freshly isolated and cultured HUVEC for determination of mRNA levels of hOCT3 and hPMAT transporters by real-time PCR. For the histamine uptake studies we used cultured HUVEC and determined [3H]histamine uptake.

Results

OCT3 and PMAT are expressed in freshly isolated HUVEC as well as in primary HUVEC culture. Ouabain (0.1 mM) had no influence on uptake of histamine. Corticosteron inhibited the uptake of histamine in HUVEC, however the effect was observed only in mM concentration.

Conclusions

Our results suggest that because of low sensitivity of histamine uptake to corticosteron, expression of OCT3 in HUVEC is probably not relevant to histamine uptake in these cells, while PMAT expression is worthy of further examination.

Acknowledgements

This work was supported by a research grant from the Ministry of Higher Education, Science and Technology (P3-067).