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This article is part of the supplement: 18th Scientific Symposium of the Austrian Pharmacological Society (APHAR)

Open Access Meeting abstract

Soluble osteopontin concentrations in serum and ascites of women with advanced serous ovarian cancer

Katarina Černe1*, Ana Bačnik1, Katarina Galič-Jerman2 and Borut Kobal2

Author Affiliations

1 Institute of Pharmacology and Experimental Toxicology, Faculty of Medicine, University of Ljubljana, 1000 Ljubljana, Slovenia

2 Division of Gynaecology, University Medical Centre Ljubljana, 1000 Ljubljana, Slovenia

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BMC Pharmacology and Toxicology 2012, 13(Suppl 1):A72  doi:10.1186/2050-6511-13-S1-A72


The electronic version of this article is the complete one and can be found online at: http://www.biomedcentral.com/2050-6511/13/S1/A72


Published:17 September 2012

© 2012 Černe et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Background

Despite advances in surgery and combination chemotherapy, ovarian cancer is first in terms of death rates of gynaecological malignancies. More than 90% of ovarian cancers arise from surface epithelium and the serous histological subtype is the most commonly diagnosed epithelial ovarian carcinoma. Extensive seeding of the peritoneal cavity by tumour cells is often associated with ascites, particularly in advanced, high-grade serous carcinomas. Currently CA-125 is the most widely used biomarker in the evaluation and management of women with epithelial ovarian cancer. However, in approximately 15% of these patients CA-125 is not indicative of disease status or progression. Therefore, an alternative tumour marker would be useful. Osteopontin is a secreted, integrin-binding glykophosphoprotein which is overexpressed in ovarian cancer cells and thus may serve as a serum biomarker. By combining the data from blood and fluid from the proximity of the tumour we might be more likely to discover a protein biomarker secreted from the tumour rather than deriving from another part of the body.

Methods

We analysed twenty patients treated at the Department of Gynaecology, Ljubljana, divided into two groups: controls (without adnexal pathology) and patients with advanced serous ovarian cancer (International Federation of Gynecology and Obstetrics (FIGO) stage III and IV). Both serum and free peritoneal fluid including ascites were collected and examined. Preoperative osteopontin concentrations were determined using the FlowCytomix Simplex kit (eBioscience). FlowCytomix Pro 2.4 (eBioscience) was used for data analysis.

Results

Patients with advanced ovarian cancer had significantly increased serum osteopontin concentration vs. controls (p < 0.013) and increased concentration of osteopontin in ascites vs. peritoneal fluid from control patients (p < 0.001).

Conclusions

Our preliminary results suggest that osteopontin might represent an effective biomarker associated with advanced serous ovarian cancer due to its elevated levels in both serum and ascites. The potential utility of osteopontin determination in monitoring women with CA-125-negative disease is worthy of exploration. However, larger prospective trials will be needed to assess the ability of serum osteopontin to provide diagnostic and prognostic information or indications of treatment response.

Acknowledgements

Supported by research grants from the Ministry of Higher Education, Science and Technology (P3-067) and the University Medical Centre Ljubljana (project no. 20110224).