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This article is part of the supplement: 18th Scientific Symposium of the Austrian Pharmacological Society (APHAR)

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Influence of different Hypericum perforatum L. preparations on pharmacokinetic and pharmacodynamic properties of pentobarbital, diazepam and paracetamol

Aleksandar Rašković*, Nebojša Stilinović and Momir Mikov

Author affiliations

Department of Pharmacology, Toxicology and Clinical Pharmacology, Faculty of Medicine, University of Novi Sad, 21000 Novi Sad, Serbia

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BMC Pharmacology and Toxicology 2012, 13(Suppl 1):A11  doi:10.1186/2050-6511-13-S1-A11

The electronic version of this article is the complete one and can be found online at:

Published:17 September 2012

© 2012 Rašković et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Herb–drug interactions are an important safety concern and the study was conducted regarding the interaction between the natural top-selling antidepressant remedy Hypericum perforatum and conventional drugs.


This study examined the influence of acute pretreatment with different H. perforatum extracts on pentobarbital-induced sleeping time impairment of motor coordination caused by diazepam and paracetamol pharmacokinetics in mice. The preparations profile of St. John’s wort was determined using RP-HPLC analysis. Ethanolic extract, aqueous extract, infusion, tablet and capsule of H. perforatum were used in the experiment.


By quantitative HPLC analysis of active principles, it has been proved that H. perforatum ethanolic extract has the largest content of naphtodianthrones: hypericin (57.8 µg/ml) and pseudohypericin (155.4 µg/ml). Pretreatment with ethanolic extract of H. perforatum potentiated the hypnotic effect of pentobarbital and the impairment of motor coordination caused by diazepam to the greatest extent and also increased the paracetamol plasma concentration in comparison to the control group. These results were in correlation to naphtodianthrones concentrations.


The obtained results show a considerable influence of H. perforatum on pentobarbital and diazepam pharmacodynamics and paracetamol pharmacokinetics.


This work was supported by the Provincial Secretariat for Science and Technological Development, Autonomous Province of Vojvodina, project no. 114-451-2458/2011.