Table 2

Description of information collected for all ionic currents
Parameter name [unit] Ionic current
ICa IKr IKs INa Description
channel type + - + + For the cell lines heterologously expressing channel subunits: name of the gene encoding expressed subunits or name of expressed channel and its origin (r-rat; h-human)
in vitro cell model + + + + Cell line used for channel subunits expression or cardiomyocytes origin (e.g. HEK, XO, guinea pig VM)
stimulation frequency [Hz] + - - + Number of current provoking pulses delivered every second expressed in Hz
transfection type + + + + Mode of channel expression in heterologous system (stable or transient)
temperature [0C] + + + + Temperature maintained during experiment
technique + + + + Technique used for current recordings
Ca2+ bath [mM] + - - - Calcium ion concentration in the bath solution
K+ bath [mM] - + + - Potassium ion concentration in the bath solution
Na+ bath [mM] - - - + Sodium ion concentration in the bath solution
t1 pulse [ms] + + + + Duration of first pulse in the step-protocol
t2 pulse [ms] + + + + Duration of second pulse in the step-protocol
holding potential [mV] + + + + The starting membrane voltage level
depolarization potential [mV] + + + + Voltage of membrane depolarizing impulse
repolarization potential [mV] + + + + Voltage of membrane repolarizing impulse
measurement potential [mV] + + + + Membrane voltage level during current amplitude measurement
protocol + + + + Type of voltage protocol applied to elicit current (e.g. step, AP, ramp)
data source + + + + First author name and publication year
PMID + + + + PubMed Unique Identifier, a unique number assigned to each PubMed record
IC50 [μM] + + + + The concentration of compound that results in 50% inhibition of current in micromolar
comments + + + + Additional information e.g. alternative naming, denotation of smiles and/or IUPAC name source if not PubChem; source of uncertainty

Where: "+” – parameter present in the record description; "-” – parameter absent in the record description.

Polak et al.

Polak et al. BMC Pharmacology and Toxicology 2012 13:6   doi:10.1186/2050-6511-13-6

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