FA intensity decreases with increasing EGF concentration and number per cell is maximal at intermediate EGF concentrations. Histograms of FA intensity A.-C. and number per cell E.-G. were generated by dividing cells into three EGF stimulation groups (A., E. no EGF (0 nM), B., F. low EGF (0.01 and 0.1 nM) and C., G. high EGF (1, 10 and 100 nM)). Histograms were fitted with lognormal probability distributions. The mean values of FA D. intensity and H. number per cell are also shown. The number of measurements of FA number per cell is the product of the average number of frames and the cell number. The number of measurements of FA properties is the product of the average FA number and the cell number. Intensity: Ncell,no = 5, NFA,no = 1963, Ncell,low = 20, NFA,low = 13,024, Ncell,high = 30, NFA,high = 14,648. Number per cell: Ncell,no = 5, NFA,no = 1545, Ncell,low = 20, NFA,low = 5909, Ncell,high = 30, NFA,high = 8937. Error bars are 95 % confidence intervals and asterisks denote p < 0.01.
Hou et al. BMC Biophysics 2012 5:8 doi:10.1186/2046-1682-5-8