Additional file 2.
Materials and Methods. This file has a description of the materials and methods for the biological samples that were used to test our software. Hippocampal neurons from 18- day- old rat embryos were cultured until 10-14 DIV on glass coverslips, according to the Banker technique  density of (~ 6000 neurons/cm2). The protocol described in Ref.  was used to label GluR2-containing AMPARs.
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Menchón et al. BMC Biophysics 2012 5:4 doi:10.1186/2046-1682-5-4