ITC profile of the EhCaBP1-Ca (A), EhCaBP1-Sr (B), and EhCaBP1-Ba (C) associations at 25°C. Upper panel– raw data for sequential 10 μL injections of 0.5 mM metals into 0.02 mM EhCaBP1 solution; Lower panel–the data points obtained by integration of the peaks in the upper panel, and plotted against the molar ratio (metal: protein). The squares are the experimental data and the solid line represents the best fit obtained from the data fitting by sequential mode. Further details are given in the materials and methods section.
Kumar et al. BMC Biophysics 2012 5:15 doi:10.1186/2046-1682-5-15