Fluorescent signals as function of loop length and loop position. Loop lengths L vary from 0 (PM) to thirteen additional thymine bases, loop positions P vary from 7 to 26. Loop position P means that the loop forming additional bases are inserted after base number P of the probe motif (counted from the surface). Strongest signal is set to 1, weakest signal is set to 0. Hybridization temperature is 317 K.
Trapp et al. BMC Biophysics 2011 4:20 doi:10.1186/2046-1682-4-20