Figure 3.

Distinct sub-mitochondrial distributions of the three hVDAC isoforms. Confocal microscopy of immunolabeled cultured human U2OS cells. Green: Different hVDAC isoforms, as indicated. For visualization, the respective Flag-tag fusion proteins were decorated with an antiserum against the Flag-peptide. Red: Mitochondria, highlighted with antibodies against the mitochondrial protein Tom20. Blue: Nucleus, stained with DAPI. The close-ups of the overlays show that hVDAC1 and hVDAC2 are localized in distinct domains, whereas hVDAC3 is uniformly distributed along the mitochondrial tubules.