Clustering of A. thaliana accessions and their corresponding transcript levels. A: AtSSI; B: AtSSII; C: AtSSIII; D: AtSSIV; E: AtGBSS. Left: Unrooted maximum likelihood tree among accessions based on promoter and gene sequences. The clusters for each gene, except AtSSII, are highlighted with circles. Right: TukeyHSD test for verification of significant differences in transcript levels among accessions. Six accessions representing different clusters of respective starch synthase were used for Realtime PCR analysis and are marked with the same color in the left and right panel. Pairwise comparisons of transcript levels with confidence levels of 95% that are different from zero indicate significant differences in transcript levels among accessions.
Schwarte et al. BMC Research Notes 2013 6:84 doi:10.1186/1756-0500-6-84