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Open Access Short Report

Rapid research response to the 2009 A(H1N1)pdm09 influenza pandemic (Revised)

Wendy A Keitel128*, Pedro A Piedra13, Robert L Atmar12, Gail Demmler3, Hana M El Sahly12, Jill Barrett4, Rebecca A Halpin5, Rosanna Lagos6, Susan Fisher-Hoch7 and Flor Munoz13

Author Affiliations

1 Department of Molecular Virology & Microbiology, Houston, TX, USA

2 Department Medicine, Houston, TX, USA

3 Department Pediatrics, Baylor College of Medicine, Houston, TX, USA

4 The EMMES Corporation, Rockville, MD, USA

5 J. Craig Venter Institute, Rockville, MD, USA

6 Centro para Vacunas en Desarrollo-Chile, Santiago, Chile

7 University of Texas School of Public Health, Brownsville, TX, USA

8 Departments of Molecular Virology & Microbiology and Medicine, Baylor College of Medicine, Room 280, One Baylor Plaza, Houston, TX 77030, USA

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BMC Research Notes 2013, 6:177  doi:10.1186/1756-0500-6-177

Published: 3 May 2013

Abstract

Background

When novel influenza viruses cause human infections, it is critical to characterize the illnesses, viruses, and immune responses to infection in order to develop diagnostics, treatments, and vaccines. The objective of the study was to collect samples from patients with suspected or confirmed A(H1N1)pdm09 infections that could be made available to the scientific community. Respiratory secretions, sera and peripheral blood mononuclear cells (PBMCs) were collected sequentially (when possible) from patients presenting with suspected or previously confirmed A(H1N1)pdm09 infections. Clinical manifestations and illness outcomes were assessed. Respiratory secretions were tested for the presence of A(H1N1)pdm09 virus by means of isolation in tissue culture and real time RT-PCR. Sera were tested for the presence and level of HAI and neutralizing antibodies against the A(H1N1)pdm09 virus.

Findings and conclusions

Thirty patients with confirmed A(H1N1)pdm09 infection were enrolled at Baylor College of Medicine (BCM). Clinical manifestations of illness were consistent with typical influenza. Twenty-eight of 30 had virological confirmation of illness; all recovered fully. Most patients had serum antibody responses or high levels of antibody in convalescent samples. Virus-positive samples were sent to J. Craig Venter Institute for sequencing and sequences were deposited in GenBank. Large volumes of sera collected from 2 convalescent adults were used to standardize antibody assays; aliquots of these sera are available from the repository. Aliquots of serum, PBMCs and stool collected from BCM subjects and subjects enrolled at other study sites are available for use by the scientific community, upon request.

Keywords:
2009 H1N1 virus; Immune responses; Influenza