Table 5

All bacteria real-time PCR detection with a universal system in 41 diarrheal stool specimens, before and after lyophilization (Ct value)
Sample n° 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30
Without lyophilization NA NA 30.54 NA 28.83 34.47 35.95 36.64 27.85 28.04 34.85 34.02 NA 30.14 26.82
With lyophilization 34.65 17.44 26.86 29.49 28.21 24.99 23.43 39.07 22.81 25.02 22.53 32.33 25.78 28.33 26.36
Sample n° 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45
Without lyophilization 33.42 26.45 34.22 NA 34.63 30.36 28.49 26.98 33.14 23.75 NA 22.79 31.95 31.05 33.50
With lyophilization 21.18 22.03 21.79 26.01 21.84 24.4 29.74 23.24 28.85 21.29 33.43 19.31 21.41 32.68 22.23
Sample n° 46 47 48 49 50 51 52 53 54 55 56
Without lyophilization 29.24 NA NA 24 23.21 23.05 17.59 19.94 14.65 18.33 18.58
With lyophilization 20.62 35.95 43.68 22.91 10.82 23.24 16.33 19.66 14.43 19.15 19

NA, not amplified. The proportion of positive specimens was significantly higher after lyophilization for the detection of Bacteria (p = 0.015).

Donatin and Drancourt

Donatin and Drancourt BMC Research Notes 2012 5:702   doi:10.1186/1756-0500-5-702

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