Table 2

M. smithii real-time PCR detection in 15 non-diarrheal stool specimens diluted in 1:5 in sterile phosphate buffer, before and after lyophilization (Cycle threshold [Ct] value)
Sample n° 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15
Without lyophilization 40.5 NA 38.93 36.43 26.38 NA 27.92 39.63 NA 22.27 33.43 43.61 38.53 NA 23.39
With lyophilization 34.39 34.74 33.93 16.97 16.84 11.72 20.16 30.24 19.76 23.11 22.65 NA 31.15 23.28 22.41

NA, not amplified.

Donatin and Drancourt

Donatin and Drancourt BMC Research Notes 2012 5:702   doi:10.1186/1756-0500-5-702

Open Data