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Expression of antigen tf and galectin-3 in fibroadenoma

Itandehui Belem Gallegos1, Eduardo Pérez-Campos2, Margarito Martinez2, Miguel Ángel Mayoral1, Laura Pérez1, Sergio Aguilar1, Edgar Zenteno3, Maria del Socorro Pina1 and Pedro Hernández1*

Author Affiliations

1 Centro de Investigaciones en Ciencias Medicas y Biológicas Facultad de Medicina, Universidad Autónoma Benito Juárez de Oaxaca, 68020, Oaxaca, Mexico

2 Unidad de Investigación en Bioquímica, Instituto Tecnológico de Oaxaca, Oaxaca, Mexico

3 Laboratorio de Inmunología, Departamento de Bioquímica, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico, 04510, Mexico

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BMC Research Notes 2012, 5:694  doi:10.1186/1756-0500-5-694

Published: 24 December 2012



Fibroadenomas are benign human breast tumors, characterized by proliferation of epithelial and stromal components of the terminal ductal unit. They may grow, regress or remain unchanged, as the hormonal environment of the patient changes. Expression of antigen TF in mucin or mucin-type glycoproteins and of galectin-3 seems to contribute to proliferation and transformations events; their expression has been reported in ductal breast cancer and in aggressive tumors.


Lectin histochemistry, immunohistochemistry, and immunofluorescence were used to examine the expression and distribution of antigen TF and galectin-3. We used lectins from Arachis hypogaea, Artocarpus integrifolia, and Amaranthus lecuocarpus to evaluate TF expression and a monoclonal antibody to evaluate galectin-3 expression. We used paraffin-embedded blocks from 10 breast tissues diagnosed with fibroadenoma and as control 10 healthy tissue samples. Histochemical and immunofluorescence analysis showed positive expression of galectin-3 in fibroadenoma tissue, mainly in stroma, weak interaction in ducts was observed; whereas, in healthy tissue samples the staining was also weak in ducts. Lectins from A. leucocarpus and A. integrifolia specificaly recognized ducts in healthy breast samples, whereas the lectin from A. hypogaea recognized ducts and stroma. In fibroadenoma tissue, the lectins from A. integrifolia, A. Hypogaea, and A. leucocarpus recognized mainly ducts.


Our results suggest that expression of antigen TF and galectin-3 seems to participate in fibroadenoma development.

Antigen TF; Galectin-3; Fibroadenoma; Breast cancer; Plant lectins