Influence of reference genes selected for normalization on the expression profile of Cnr1 one week after FS induction.Cnr1 expression levels were normalized by geometric averaging of three (A) or four (B) stably expressed genes as identified by geNorm and Normfinder. 18S rRNA and ActB, indicated by geNorm and Normfinder as the least stable genes, are used for normalization of Cnr1 expression data (C). Normothermia controls (n = 9); FS+, hyperthermia with febrile seizures (n = 7). Data are presented as mean ± SEM. *, P < 0,05; analyzed using a Mann–Whitney test.
Swijsen et al. BMC Research Notes 2012 5:685 doi:10.1186/1756-0500-5-685