Evaluation of candidate reference genes using geNorm analysis software. A: Average expression stability measure (M) of reference genes in the total sample set (n = 22), analyzed by stepwise exclusion of the least stable reference gene. B: Determination of the optimal number of reference genes for normalization by means of pair-wise variation (Vn/n+1) analysis. Every bar represents the pairwise variation (V) in normalization accuracy when stepwise adding reference genes according to the ranking in A.
Swijsen et al. BMC Research Notes 2012 5:685 doi:10.1186/1756-0500-5-685