Walking along the growing sequence. The growing CaM-kinase sequence is shown using a similar display to Figure 1 during a walk towards the 3’ end. However, the upper target sequence is now the melded sequence from all of the discovered reads. Individual steps in the walk correspond to repeated complete searches through both paired end files, following which the newly discovered matching sequences are melded onto the end of the growing sequence. Several errors in sequencing and matching are evident, but the large numbers of overlapping reads allow quality-based voting in the melding process.
French BMC Research Notes 2012 5:673 doi:10.1186/1756-0500-5-673