Table 3

DNA primers used in quantity qPCR assays
Organism Gene target (amplicon size)a Name Sequence Conb AEc Ref.
E. coli lacZ (70) lacZF1 CCT GAG GCC GAT ACT GTC GT 3 0.99 [52]
lacZR1 TTG GTG TAG ATG GGC GCA T 3
B. thailandensis fliC (62) fliCF1 AGC AGA TCT CGG AAG TGA ACC 2 0.92 This study
fliCR1 GAG GAT GTT CTT GCC GTT GT 2
B. cereus pc-plc (144) PCER-F GGA TTC ATG GAG CGG CAG TA 3 0.95 [53]
PCER-3R GCT TAC CTG TCA TTG GTG TAA CTT CA 2
S. cerevisiae 26Sd (124) YEAST-F GAG TCG AGT TGT TTG GGA ATG C 3 0.91 [54]
YEST-R TCT CTT TCC AAA GTT CTT TTC ATC TTT 2

a Amplicon size in base pairs.

b Optimized primer concentration (μM).

c Amplification Efficiency, calculated using the slope of the standard curve.

d Assay targeted the D1/D2 variable region of 26S.

Olson and Morrow

Olson and Morrow BMC Research Notes 2012 5:668   doi:10.1186/1756-0500-5-668

Open Data