Email updates

Keep up to date with the latest news and content from BMC Research Notes and BioMed Central.

Open Access Research article

DNA extract characterization process for microbial detection methods development and validation

Nathan D Olson and Jayne B Morrow*

Author Affiliations

Biosystems and Biomaterials Division, Material Measurements Laboratory, National Institute of Standard and Technology, 100 Bureau Dr, 20899-8312, Gaithersburg, Maryland

For all author emails, please log on.

BMC Research Notes 2012, 5:668  doi:10.1186/1756-0500-5-668

Published: 3 December 2012

Additional files

Additional file 1:

Table S1. DNA A260/A280 and A260/A230 absorbance ratios.

Format: DOC Size: 43KB Download file

This file can be viewed with: Microsoft Word Viewer

Open Data

Additional file 2:

Figure S1. Scatter Plot of A260/A280 and A260/A230 Ratios as a Function of a Control DNA (SRM 2372) Concentration. The experiments were executed on two separate days, A and B: with single (4 runs each) and triple (4 runs each) replicates respectively. The data point shape indicates day A, ●, B, ▲. The red vertical lines indicate the DNA concentration cutoff used for analysis of the DNA extract purity at 17.5 ng/μL.

Format: PDF Size: 113KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data

Additional file 3:

Figure S2. Electropherograms for DNA Extracts Grouped by Cell Type and Extraction Method. The two manufacturer supplied markers are at 50 bp and 17000 bp. Dotted lines indicate the 1000 bp point on the x-axis. The bottom number within each graph is the number of replicates where the area under the curve for the full electropherogram was above the analysis threshold. The top two numbers are the mean and standard deviation for the percentage of DNA that was greater than 1000 bp (top value) and less than 300 bp (middle value). Scales are independent for each row due to the large range in responses.

Format: PDF Size: 1.5MB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data

Additional file 4:

Figure S3. Measured DNA concentration as a Function of the dilutions of the Human Quantification Standard (SRM 2372) concentration (ng/μL). Three dilution series replicates were processed, indicated in red, green, and blue, with five runs each. Dotted line indicates the defined limit of quantification (0.25 ng/μL) based on the increased observed variability for lower concentration dilutions. (PDF 94 kb)

Format: PDF Size: 94KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data

Additional file 5:

Raw data (sheet labeled "DNAcompleteResults.csv") and metadata (sheet labeled "Head descriptions") used to support the results presented in this article.

Format: XLSX Size: 30KB Download file

Open Data