Table 1

Bacterial strains and their corresponding collection number used to test sensitivity and specificity of the different primer pairs
Species Strain Origin
Actinobaculum schaalii TSW25BA12a human, vagina
Actinomyces meyeri PB2003/218-T1-6a human, vagina
Actinomyces naeslundii CCUG 18310T human, sinus
Actinomyces neuii TSW23BA4a human, vagina
Actinomyces odontolyticus LMG 15953 human, drain after lung resection
Actinomyces turicensis TSW24BA1a human, vagina
Aggregatibacter actinomycetemcomitans DSM 11123 human, subgingival dental plaque
Agrobacterium radiobacter 0106 0380a not recorded
Bacteroides fragilis CCUG 4856T, 03L2177a human, appendix abscess;
Bacteroides thetaiotaomicron CCUG 34778 human, appendix
Fusobacterium nucleatum CCUG 32989T human, cervico-facial lesion
Fusobacterium varium DSM 19868T human, faeces
Parvimonas micros CCUG 46357T human, purulent pleurisy
Peptostreptococcus anaerobius FWOBV0180a not recorded
Porphyromonas gingivalis CCUG 25893T human, gingival sulcus
Porphyromonas somerae VMF0235S33 human, vagina
Prevotella melaninogenica FWO BV0747a human, vagina
Prevotella bivia FWO BV0913a human, vagina
Prevotella buccalis FWO BV0754a human, vagina
Prevotella disiens VMF 1000SRT31 human, vagina
Prevotella corporis TSW04CA1a human, vagina
Prevotella intermedia CCUG 24041T human, empyema
Streptococcus agalactiae LMG 14694T bovine, milk
Streptococcus anginosus LMG 14502T human, throat
Streptococcus mitis LMG 14557T human, oral cavity
Streptococcus mutans LMG 14558T human, carious dentine
Streptococcus oralis LMG 14532T human, oral cavity
Streptococcus pneumoniae LMG 14545T not recorded
Streptococcus pyogenes LMG 14700T not recorded
Streptococcus sanguinis LMG 14702T human, subacute bacterial endocarditis
Streptococcus salivarius LMG 11489T human, blood
Streptococcus sobrinus LMG14641T human, dental plaque
Tannerella forsythia CCUG 21028AT Human, periodontal pocket
Treponema denticola Oligob not applicable

Legend

a: Patient isolate.; b: T. denticola could not be cultured. Therefore, a ds oligonucleotide was used as template for preparing the standard series.

Decat et al.

Decat et al. BMC Research Notes 2012 5:664   doi:10.1186/1756-0500-5-664

Open Data