Analysis by mFold of the secondary structure of the Tannerella forsythia 16S rRNA gene amplicon, targeted by the primers described by Kuboniwa et al. . Folding conditions were adapted to qPCR conditions (see 2.4). Forward primer anneals on bp 1–22 region, which contains a hairpin (bp 7–18).
Decat et al. BMC Research Notes 2012 5:664 doi:10.1186/1756-0500-5-664