Confirmation of integration of a full-length tga3Q207L gene in ectopic co-transformants 3/3 and 4/5. The presence of a complete copy of the tga3Q207L gene in co-transformants 3/3 (lane 2) and 4/5 (lane 3) was confirmed by PCR using primers tga3PromFw, which binds to the 5′ non-coding region of tga3, and M13Fw, which binds to the backbone of plasmid pKAtga3 (Table 1). Both co-transformants yielded the expected 3343-bp amplicon. Lane 1, wild-type; lane 4, plasmid pKAtga3.
Gruber et al. BMC Research Notes 2012 5:641 doi:10.1186/1756-0500-5-641