Screening of T. atroviride co-transformants for the presence of the nptII gene. Genomic DNA was isolated from 22 geneticin-resistant transformants resulting from co-transformation of T. atroviride P1 with plasmids pKAtga3 and pII99 and analyzed by PCR for integration of the nptII gene using primers pII99Fw and pII99Rev. M, DNA size standard; +, plasmid pII99; 1–22, transformants; -, wild-type.
Gruber et al. BMC Research Notes 2012 5:641 doi:10.1186/1756-0500-5-641