Human recombinant TGF-β1 (hTGF-β1) - luciferase standard curve. PAI-1 promoter/luciferase construct-transfected MLECs were incubated with various concentration of human recombinant TGF-β1 at 37°C for 20 h. There is a strict dose-dependent increase in luciferase activity (RLU) by TGF-β1 between 0 and 0.1 ng/ml. The values represents mean ± SD of 5 individual experiments. MLECs=mink lung epithelial cells, PAI=plasminogen activator inhibitor, RLU=relative luciferase unit, SD=standard deviation, TGF-β=transforming growth factor-β.
Khan et al. BMC Research Notes 2012 5:636 doi:10.1186/1756-0500-5-636