Table 1

LC-MALDI-TOF-TOF results
Workflow No. of MS Spectraa No. of total MS/MS Spectra No. of MS/MS Spectra (mean of all LC-run) Total identified Peptides Non redundant identified peptidesb Mean of proteins ± SD (n total protein)c MS/MS efficiency (%)d Peptides/ Proteins ± SD Proteins identified by a single peptide (%)
Anionic 125 7100 888 2700 1305 163 ± 87.6 (326) 38% 3.8 ± 4.3 15 (4,6%)
Cationic 125 5832 729 1432 718 94 ± 39.6 (188) 26% 3.9 ± 4.4 11 (1,5%)
Lipophilic 125 6554 819 1480 691 99 ± 5.6 (198) 23% 4.0 ± 4.2 9 (1,3%)

a) number of total MS spectra/workflow; b) Peptide counts are based on non- redundant identifications in technical replicates and/or in redundant acquisitions of the same sample; c) Mean ± SD of identified proteins in four and three technical replicates for SPE-LC-MS/MS analysis and in brackets number of total identified proteins; d) Efficiency is determined dividing the number of identified peptides per MS/MS spectra.

Boccardi et al.

Boccardi et al. BMC Research Notes 2012 5:612   doi:10.1186/1756-0500-5-612

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