Open Access Technical Note

An automated plasma protein fractionation design: high-throughput perspectives for proteomic analysis

Claudia Boccardi12, Silvia Rocchiccioli1, Antonella Cecchettini13, Alberto Mercatanti1 and Lorenzo Citti1*

Author Affiliations

1 Institute of Clinical Physiology-CNR, Via Moruzzi 1, 56124 Pisa, Italy

2 Center for Nanotechnology Innovation @NEST, Istituto Italiano di Tecnologia, Piazza San Silvestro 12, 56127 Pisa, Italy

3 Department of Human Morphology and Applied Biology, University of Pisa, Via Roma 55 Pisa, Italy

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BMC Research Notes 2012, 5:612  doi:10.1186/1756-0500-5-612

Published: 1 November 2012

Additional files

Additional file 1:

Supporting figures and tables Additional information about results.

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Additional file 2:

Supporting materials and methods. Additional information about solvents, volumes and conditions used for the home-designed robotic platform.

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Additional file 3:

Supporting Table 1. Proteins identified in the acid fraction. Protein name, accession number, pI and other additional information are reported.

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Additional file 4:

Supporting Table 2. Proteins identified in the basic fraction. Protein name, accession number, pI and other additional information are reported.

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Additional file 5:

Supporting Table 3. Proteins identified in the lipophilic fraction. Protein name, accession number, pI and other additional information are reported.

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