Open Access Short Report

Human blood RNA stabilization in samples collected and transported for a large biobank

Nur Duale1*, Gunnar Brunborg1, Kjersti S Rønningen14, Thomas Briese2, Jeanette Aarem1, Kaja K Aas1, Per Magnus1, Camilla Stoltenberg1, Ezra Susser23 and W Ian Lipkin2

Author Affiliations

1 The Norwegian Institute of Public Health, Oslo, Norway

2 The Mailman School of Public Health, Columbia University, New York, NY, USA

3 New York State Psychiatric Institute, New York, NY, USA

4 Present address: Oslo University Hospital, Rikshospitalet, Oslo, Norway

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BMC Research Notes 2012, 5:510  doi:10.1186/1756-0500-5-510

Published: 18 September 2012

Additional files

Additional file 1:

The non-normalized raw Cq-values. A) The non-normalized raw Cq-values for adult blood samples collected in the PAXgene tubes; B) The non-normalized raw Cq-values for adult blood samples collected in the Tempus tubes; C) The non-normalized raw Cq-values for cord blood samples collected in the PAXgene and in the Tempus tubes. Each bar represents the average Cq-values and the error bar indicates ± SE.

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Additional file 2:

Temperature recordings for transported samples vs RIN, RNA yield and Raw Cq-value. Temperatures were monitored during the transportation of the samples to four different locations (hospitals) in Norway. The four samples were sent by standard mail at February 21th and all samples returned to NIPH at February 24th. Transportation time for samples sent to Bærum, Kristiansand and Bergen was ~ 48 hours, while it took ~ 74 hours for Kirkenes samples to return to NIPH. The temperature variations ranged from 3.4°C to 27.1°C, and none of the samples were frozen during the transportation. A) Temperature recordings for samples sent to the four locations and samples kept at NIPH; B) RIN values vs. average temperature (°C) of the samples; C) RNA yields vs. average temperature (°C) of the samples; D) The non-normalized raw Cq-values vs. average temperature (°C) of the samples. Each bar represents the average Cq-values and the error bar indicates ± SE for the raw Cq-values or ± SD for average temperature (°C) of the samples.

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Additional file 3:

The non-normalized raw Cq-values for suboptimal blood volume QC. A) Evaluation of the non-normalized raw Cq-values for suboptimal blood volume QC from adult blood samples; and B) Evaluation of the non-normalized raw Cq-values for suboptimal blood volume QC from cord blood samples. Each bar represents the average Cq-values and the error bar indicates ± SE.

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Additional file 4:

The evaluation of 18S rRNA stability. A) Comparison of PAXgene tube-collected samples against the Tempus tube-collected samples: The Cq – values for 18S rRNA were relatively stable following the storage of the tubes for up to seven days at room temperature for both tubes; B) Evaluation of 18S rRNA stability for transportation QC samples: The Cq – values for 18S rRNA were relatively stable following the transportation; C) Suboptimal blood volume in the Tempus tubes on 18S rRNA expression stability for adult blood samples: Suboptimal blood volume collected in the tube has a significant effects on 18S rRNA expression stability; and D) Suboptimal blood volume in the Tempus tubes on 18S rRNA expression stability for cord blood samples: Suboptimal blood volume collected in the tube has some effects on 18S rRNA expression stability. Each bar represents the average Cq-values and the error bar indicates ± SE.

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