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Open Access Technical Note

LogSpin: a simple, economical and fast method for RNA isolation from infected or healthy plants and other eukaryotic tissues

Hila Yaffe1, Kobi Buxdorf1, Illil Shapira2, Shachaf Ein-Gedi2, Michal Moyal-Ben Zvi2, Eyal Fridman2, Menachem Moshelion2 and Maggie Levy1*

Author Affiliations

1 Department of Plant Pathology and Microbiology, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel

2 The Robert H. Smith Institute of Plant Sciences, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel

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BMC Research Notes 2012, 5:45  doi:10.1186/1756-0500-5-45

Published: 19 January 2012

Abstract

Background

Rapid RNA extraction is commonly performed with commercial kits, which are very expensive and can involve toxic reagents. Most of these kits can be used with healthy plant tissues, but do not produce consistently high-quality RNA from necrotic fungus-infected tissues or fungal mycelium.

Findings

We report on the development of a rapid and relatively inexpensive method for total RNA extraction from plants and fungus-infected tissues, as well as from insects and fungi, based on guanidine hydrochloride buffer and common DNA extraction columns originally used for the extraction and purification of plasmids and cosmids.

Conclusions

The proposed method can be used reproducibly for RNA isolation from a variety of plant species. It can also be used with infected plant tissue and fungal mycelia, which are typically recalcitrant to standard nucleic acid extraction procedures.

Keywords:
RNA extraction; Infected tissue; Plant; Fungus; Aphids