Figure 3.

Immunostaining of PIWIL1 protein in NCI-H1299 lung cancer cell lines stably transduced with either MLV-backbone (NCI-H1299-BB, A) or MLV-HA-RASSF1C (NCI-H1299-1C, B). Cells were immuno-stained using PIWIL1 antibody as described in the Methods section. The PIWIL1 protein staining is more pronounced in NCI-H1299-1C over-expressing RASSF1C compared to NCI-1299-BB (control) cells clearly demonstrating that RASSF1C over-expression up-regulates PIWIL1 gene expression.

Reeves et al. BMC Research Notes 2012 5:239   doi:10.1186/1756-0500-5-239
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